Significant cholinergic role in secretin-stimulated exocrine secretion in isolated rat pancreas

Park, Hyung Seo; Lee, Yun Lyul; Kwon, Hyeok Yil; Chey, William Y.; Park, Hyoung Jin

doi:10.1152/ajpgi.1998.274.2.g413

Cited by 14 publications

(25 citation statements)

References 21 publications

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“…4). This scenario would explain the long-standing observation in pancreatic physiology-the fact that in the intact pancreas the stimulation of acini potentiates the effect of secretin on the production of the HCO À 3 -rich juice [164,165]. Figure 4 shows the working model for how ATP, and/or hydrolytic products as well as the associated enzymes and receptors, could play a role as auto/paracrine regulators in the pancreas.…”

Section: Integrated Pancreatic Functionmentioning

confidence: 98%

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Purinergic receptors in the endocrine and exocrine pancreas

Novak

2007

Purinergic Signalling

115

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The pancreas is a complex gland performing both endocrine and exocrine functions. In recent years there has been increasing evidence that both endocrine and exocrine cells possess purinergic receptors, which influence processes such as insulin secretion and epithelial ion transport. Most commonly, these processes have been viewed separately. In β cells, stimulation of P2Y 1 receptors amplifies secretion of insulin in the presence of glucose. Nucleotides released from secretory granules could also contribute to autocrine/paracrine regulation in pancreatic islets. In addition to P2Y 1 receptors, there is also evidence for other P2 and adenosine receptors in β cells (P2Y 2 , P2Y 4 , P2Y 6 , P2X subtypes and A 1 receptors) and in glucagon-secreting α cells (P2X 7 , A 2 receptors). In the exocrine pancreas, acini release ATP and ATP-hydrolysing and ATP-generating enzymes. P2 receptors are prominent in pancreatic ducts, and several studies indicate that P2Y 2 , P2Y 4 , P2Y 11 , P2X 4 and P2X 7 receptors could regulate secretion, primarily by affecting Cl − and K + channels and intracellular Ca 2+ signalling. In order to understand the physiology of the whole organ, it is necessary to consider the full complement of purinergic receptors on different cells as well as the structural and functional relation between various cells within the whole organ. In addition to the possible physiological function of purinergic receptors, this review analyses whether the receptors could be potential therapeutic targets for drug design aimed at treatment of pancreatic diseases.

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Section: Integrated Pancreatic Functionmentioning

confidence: 98%

“…Functionally, several islet hormones affect acinar and ductal secretion [166], e.g. insulin potentiates secretin-stimulated secretion in the rat pancreas [165]. Therefore, it is possible that ATP released from β cells could affect closely apposed ductal epithelium.…”

Section: Integrated Pancreatic Functionmentioning

confidence: 99%

Purinergic receptors in the endocrine and exocrine pancreas

Novak

2007

Purinergic Signalling

115

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“…EFS on rat pancreas enhanced pancreatic exocrine secretion by activation of the intrapancreatic nervous system [8,9]. Pre-treatment of atropine partly blocked this effect, but pre-treatment of tetrodotoxin, a nerve blocker, perfectly blocked it [10]. These results suggested that the non-cholinergic nerve system exists in the intrapancreatic nerve system, and it could affect the pancreatic exocrine secretion.…”

Section: Introductionmentioning

confidence: 87%

“…The isolated pancreas was prepared according to a method described previously [10]. The rats were anesthetized with a single injection of urethane.…”

Section: Preparation Of the Isolated Perfused Pancreasmentioning

confidence: 99%

Roles of Non-cholinergic Intrapancreatic Nerves, Serotonergic Nerves, on Pancreatic Exocrine Secretion in the Isolated Perfused Rat Pancreas

Jiang

Shin

Kim

et al. 2011

Korean J Physiol Pharmacol

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It has been rereported that axons which display 5-hydroxytryptamine (5-HT) immunoreactivity are abundant in the pancreas and the majority of serotonergic axons terminate within intrapancreatic ganglia, islet and acini. This histological result strongly suggests that intrapancreatic serotonergic nerves could affect to the pancreatic endocrine and exocrine secretion. Thus, this study was aimed to investigate whether intrapancreatic serotonergic nerves could affect pancreatic exocrine secretion and an action mechanism of the intrapancreatic serotonergic nerves. The rats were anesthetized with a single injection of urethane. The median line and the abdominal aorta was carefully dissected and cannulated with PE-50 tubing just above the celiac artery, and then tightly ligated just below the superior mesenteric artery. The pancreatic duct was also cannulated with Tygon microbore tubing. W ith the addition of serotonin, pancreatic volume flow and amylase output were significantly inhibited electrical field stimulation (EFS). On the other hand, pancreatic volume flow and amylase output were significantly elevated in EFS with the addition of spiperone. EFS application, however, pancreatic volume flow and amylase output had no significant change in cholecystokinin (CCK) alone when serotonin was applied under a 5.6 mM glucose background. Pancreatic volume flow and amylase output under 18 mM glucose background were significantly elevated in CCK plus serotonin than in CCK alone. These data suggest that intrapancreatic serotonergic nerves play an inhibitory role in pancreatic exocrine secretion and an important role in the insulin action or release.

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“…Even cholinergic stimulation and CCK can evoke HCO 3 2 secretion in some species, and they can potentiate the secretin effect on the volume of secretion (You et al 1983;Holst 1993;Park et al 1998;Chey and Chang 2001), although there are exceptions (Evans et al 1996). Whether human pancreatic juice is also rich in HCO 3 2 under these conditions is not clear.…”

Section: Regulation Of Pancreatic Duct Secretionmentioning

confidence: 99%

The Cystic Fibrosis of Exocrine Pancreas

Wilschanski

Novak

2013

Cold Spring Harbor Perspectives in Medicine

130

113

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The cystic fibrosis transmembrane conductance regulator (CFTR) protein is highly expressed in the pancreatic duct epithelia and permits anions and water to enter the ductal lumen. This results in an increased volume of alkaline fluid allowing the highly concentrated proteins secreted by the acinar cells to remain in a soluble state. This work will expound on the pathophysiology and pathology caused by the malfunctioning CFTR protein with special reference to ion transport and acid-base abnormalities both in humans and animal models. We will also discuss the relationship between cystic fibrosis (CF) and pancreatitis, and outline present and potential therapeutic approaches in CF treatment relevant to the pancreas.

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Significant cholinergic role in secretin-stimulated exocrine secretion in isolated rat pancreas

Cited by 14 publications

References 21 publications

Purinergic receptors in the endocrine and exocrine pancreas

Purinergic receptors in the endocrine and exocrine pancreas

Roles of Non-cholinergic Intrapancreatic Nerves, Serotonergic Nerves, on Pancreatic Exocrine Secretion in the Isolated Perfused Rat Pancreas

The Cystic Fibrosis of Exocrine Pancreas

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