Significant association of inflammation grade with the number of Langerhans cells in odontogenic keratocysts

Abstract

A significant association of inflammation grade with the number of LCs in OKCs is found. The paucity of finding LCs in the lining epithelia of OKCs without inflammation indicates the loss of immunosurveillance ability against the OKC lining epithelial cells; this can explain why OKCs have aggressive clinical behavior, a great growth potential, and a high recurrence rate.

Langerhans cells in 60 odontogenic keratocysts

“…To find out whether there were differences in the LC counts in the lining epithelia and subepithelial connective tissues of OKCs stained with either anti-CD1a or anti-S100 antibody, we compared the mean CD1a-positive LC counts with the previously-published mean S100-positive LC counts in each of three different groups of OKC with severe, mild/moderate, and no inflammation in the focal fibrous cystic walls 25 . In both the mild/moderate and no inflammation groups, the mean CD1a-positive LC counts in the subepithelial connective tissue (2.5 ± 2.7 and 0.2 ± 0.3 cells/HPF, respectively) were significantly lower than the mean S100-positive LC counts (5.0 ± 3.5 and 0.7 ± 0.6 cells/HPF, respectively) in the subepithelial connective tissue (both P-values < 0.001) (Table 2).…”

“…In general, anti-S100 immunostaining can detect a little bit more LCs in OKCs than anti-CD1a immunostaining (Table 2). 25 The CD1a is a relatively specific marker of the LC but the sensitivity of anti-CD1a antibody is slightly lower than that of anti-S100 antibody. S100 protein is not only a marker of LC but also markers of melanocytes, Schwann cells, and myoepithelial cells 26 .…”

“…The mean LC counts in the lining epithelia or in the subepithelial connective tissues of OKC samples were compared first among 3 groups by analysis of variance (ANOVA) and then between any 2 groups by Student's t -test. The mean CD1a-positive LC counts were compared with the previously-published mean S100-positive LC counts in each of three different groups of OKC with severe, mild/moderate, and no inflammation by Student's t -test 25 . The correlation between LC counts in the lining epithelia or in the subepithelial connective tissues of OKC samples and clinicopathological parameters of OKC patients was analyzed by Student's t -test.…”

Single-Cell RNA Sequencing Reveals CXCLs Enriched Fibroblasts Within Odontogenic Keratocysts

“… 7 The thicker lining epithelia was also found in OKC with focal inflammation sites than those without inflammation. 8 Therefore, inflammation might be closely related with the focal proliferation of epithelia. Recent years, chemokines are thought to be involved in endothelial cells functions especially angiogenesis.…”