Significance to investigate the TH1 /TH2 cytokines and their proportions in peripheral blood of women with unexplained recurrent spontaneous abortion and normal women based on pregnancy status

Abstract

Background: To investigate the significance of th1/th2 cytokines in the pathogenesis of unexplained recurrent spontaneous abortion(URSA) patients. Methods: Flow cytometry was used to determine the level of th1/th2 cytokines in peripheral blood of URSA patients, comparison between two groups was analysed by Mann-Whitney U test, and Kruskal-Wallis H test was used to test three or more groups. Results : T he content of il-6 in peripheral blood of adverse pregnancy group (ad-pregnancy group) was significantly higher than that of URSA group and pregnancy group. The ratio of il-2 / il-4 and IFN-γ/il-4 in peripheral blood of URSA group was significantly higher than that of pregnancy group, the ratio of IFN-γ/il-10 and IFN-γ/il-4 in peripheral blood of ad-pregnancy group was significantly higher than that of pregnancy group. The AUC of IFN-γ/il-4 was 0.821, with high diagnostic efficiency, sensitivity as high as 84.09% and specificity as high as 69.05%, which were higher than other indicators. Conclusion : The Variable of IFN-γ/il-4 can be used for the initial diagnosis of Ursa to reduce the rate of missed diagnosis, the cytokine ratio in the Th1/Th2 immune response was more important than the expression of single cytokine.