Significance of urinary type IV collagen in patients with diabetic nephropathy using a highly sensitive one‐step sandwich enzyme immunoassay

Abstract

Urinary concentrations of type IV collagen in patients with diabetic nephropathy were measured by a highly sensitive, one-step sandwich enzyme immunoassay. Samples from 298 patients with noninsulin-dependent diabetes mellitus (NIDDM) and 80 healthy controls were examined. In diabetic patients with macroalbuminuria or renal insufficiency, the concentrations of urinary type IV collagen were significantly higher than those of diabetic patients with normoalbuminuria or healthy controls (P < 0.001). Urinary type IV collagen concentration in diabetic patients with microalbuminuria was significantly higher than that in diabetic patients with normoalbuminuria or that in healthy controls (P < 0.001). In contrast, there were no significant changes in the concentration of serum type IV collagen between microalbuminuric patients and normoalbuminuric patients. The area under the receiver operating characteristic (ROC) curve for the urinary type IV collagen concentration was equivalent to that of urinary albumin. It was concluded that urinary type IV collagen concentration determined using this method might be a useful marker for the early detection of diabetic nephropathy.

Urinary biomarkers involved in type 2 diabetes: a review

“…A human biopsy study has confirmed the link between increased type IV collagen production or excretion, and increased mesangial expansion in type 2 diabetes 176. Urinary excretion of type IV collagen has been reported to increase significantly in microalbuminuric diabetic patients compared with normoalbuminuric patients and/or controls 10, 177. Together with a parallel gradual increase 91 and a significant correlation 10, 16, 91, 175 between type IV collagen excretion and UAE, these results suggest the potential usefulness of type IV collagen as urinary marker of diabetic renal disease, as reported in numerous studies 10, 16, 91, 173–177.…”

Effect of tranilast in early-stage diabetic nephropathy

“…To determine the effect of tranilast, we investigated the excretion of U-A and U-IV, which have been reported to be useful markers for DN [27][28][29][30]. Excretion of both U-A and U-IV tended to decrease in the T(þ) group and increase in the T(À) group, although the difference was not significant.…”

Evidence linking glycated albumin to altered glomerular nephrin and VEGF expression, proteinuria, and diabetic nephropathy

“…* P < 0.05 vs. db/m; # P < 0.05 vs. db/db control. mesangial expansion, glomerular occlusion, and loss of filtration function in both human and rodent diabetes [28][29][30][31][32][33]. TGF-b1 content in the renal cortex harvested at the end of the experimental period was significantly higher in tissue from diabetic controls compared to nondiabetic animals.…”