Significance of the RBD mutations in the SARS-CoV-2 Omicron: from spike opening to antibody escape and cell attachment

Hossen, Md Lokman; Baral, Prabin; Sharma, Tej; Gerstman, Bernard S.; Chapagain, Prem P.

doi:10.1101/2022.01.21.477244

Cited by 7 publications

(4 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Emerging data also suggests that the mutations within the Omicron variant facilitate a receptor-binding domain that allows more accessibility to the RGD motif, which may ultimately enhance integrin binding ( Hossen et al., 2022 ) and be associated with higher transmission rates than other variants of concern. Mapping the tissue distribution of integrins also highlights its potential as an alternative receptor.…”

Section: Integrins Are Receptors For Sars-cov-2mentioning

confidence: 99%

Integrins as Therapeutic Targets for SARS-CoV-2

Gressett

Nader²,

Robles

et al. 2022

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

Section: Integrins Are Receptors For Sars-cov-2mentioning

confidence: 99%

Integrins as Therapeutic Targets for SARS-CoV-2

Gressett

Nader²,

Robles

et al. 2022

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

“…Notably, in situ , this second hydrophobic patch is primarily exposed when the full S protein RBD is in the “up” position (Fig. 1a), and residues like S383 form hydrogen bonds with surrounding S trimer domains 50,51 . This report agrees with other studies that have also introduced mutations near the RBD core, leading to increased production and stability 52,53 .…”

Section: Discussionmentioning

confidence: 99%

Molecular engineering of a cryptic epitope in Spike RBD improves manufacturability and neutralizing breadth against SARS-CoV-2 variants

Rodriguez-Aponte

Dalvie

Wong

et al. 2022

Preprint

View full text Add to dashboard Cite

There is a continued need for sarbecovirus vaccines that can be manufactured and distributed in low- and middle-income countries (LMICs). Subunit protein vaccines are manufactured at large scales at low costs, have less stringent temperature requirements for distribution in LMICs, and several candidates have shown protection against SARS-CoV-2. We previously reported an engineered variant of the SARS-CoV-2 Spike protein receptor binding domain antigen (RBD-L452K-F490W; RBD-J) with enhanced manufacturability and immunogenicity compared to the ancestral RBD. Here, we report a second-generation engineered RBD antigen (RBD-J6) with two additional mutations to a hydrophobic cryptic epitope in the RBD core, S383D and L518D, that further improved expression titers and biophysical stability. RBD-J6 retained binding affinity to human convalescent sera and to all tested neutralizing antibodies except antibodies that target the class IV epitope on the RBD core. K18-hACE2 transgenic mice immunized with three doses of a Beta variant of RBD-J6 displayed on a virus-like particle (VLP) generated neutralizing antibodies (nAb) to nine SARS-CoV-2 variants of concern at similar levels as two doses of Comirnaty. The vaccinated mice were also protected from challenge with Alpha or Beta SARS-CoV-2. This engineered antigen could be useful for modular RBD-based subunit vaccines to enhance manufacturability and global access, or for further development of variant-specific or broadly acting booster vaccines.

show abstract

“…In contrast, substitution of the Omicron spikes results in a predominantly closed conformation that may allow them to evade antibodies (Calvaresi et al, 2023). Other studies show that the mutations in the RBD of Omicron may promote the conformation to change from "down" to "up" and thus increase engagement of ACE2 (Hossen et al, 2022;Ye et al, 2022). This may due to the mutations that reduce the protein-protein interaction affinity of RBD with its neighboring domains (Singh et al, 2022).…”

Section: Spike Mutations In Rbd Conformationmentioning

confidence: 99%

Current Progress in Genomic and Genetic Research on Human Viral Diseases

2024

Frontiers Research Topics

View full text Add to dashboard Cite

Frontiers is more than just an open access publisher of scholarly articles: it is a pioneering approach to the world of academia, radically improving the way scholarly research is managed. The grand vision of Frontiers is a world where all people have an equal opportunity to seek, share and generate knowledge. Frontiers provides immediate and permanent online open access to all its publications, but this alone is not enough to realize our grand goals. Frontiers journal seriesThe Frontiers journal series is a multi-tier and interdisciplinary set of openaccess, online journals, promising a paradigm shift from the current review, selection and dissemination processes in academic publishing. All Frontiers journals are driven by researchers for researchers; therefore, they constitute a service to the scholarly community. At the same time, the Frontiers journal series operates on a revolutionary invention, the tiered publishing system, initially addressing specific communities of scholars, and gradually climbing up to broader public understanding, thus serving the interests of the lay society, too. Dedication to qualityEach Frontiers article is a landmark of the highest quality, thanks to genuinely collaborative interactions between authors and review editors, who include some of the world's best academicians. Research must be certified by peers before entering a stream of knowledge that may eventually reach the public -and shape society; therefore, Frontiers only applies the most rigorous and unbiased reviews. Frontiers revolutionizes research publishing by freely delivering the most outstanding research, evaluated with no bias from both the academic and social point of view. By applying the most advanced information technologies, Frontiers is catapulting scholarly publishing into a new generation. What are Frontiers Research Topics?Frontiers Research Topics are very popular trademarks of the Frontiers journals series: they are collections of at least ten articles, all centered on a particular subject. With their unique mix of varied contributions from Original Research to Review Articles, Frontiers Research Topics unify the most influential researchers, the latest key findings and historical advances in a hot research area.

show abstract

Significance of the RBD mutations in the SARS-CoV-2 Omicron: from spike opening to antibody escape and cell attachment

Cited by 7 publications

References 25 publications

Integrins as Therapeutic Targets for SARS-CoV-2

Integrins as Therapeutic Targets for SARS-CoV-2

Molecular engineering of a cryptic epitope in Spike RBD improves manufacturability and neutralizing breadth against SARS-CoV-2 variants

Current Progress in Genomic and Genetic Research on Human Viral Diseases

Contact Info

Product

Resources

About