Significance of the crystal structure of cellulose in the production and action of cellulase

Rautela, Gopal S.; King, Kendall W.

doi:10.1016/0003-9861(68)90180-x

Cited by 36 publications

(15 citation statements)

References 8 publications

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“…With the culture filtrate from Chaeromium thertnophile var. dissiturn the activation energies were reported to be 6.53 and 5.33 kcal mol-' with CMC and cotton, respectively, as substrates (Eliksen and Goksoyr 1977) and 5.92-8.02 kcal mol-I for different crystalline cellulose preparations for the T. viride system (Rautela and King 1968). Injrlence…”

Section: Resultsmentioning

confidence: 95%

Cellulase and β-glucosidase production by a basidiomycete species

Shewale¹,

Sadana²

1978

Can. J. Microbiol.

117

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The optimisation of cellulase and beta-glucosidase production by a basidiomycete species was studied and cellulase and cellobiase production by this and Trichoderma viride (and its mutants) in shake flasks were compared. The former produced an active cellulase comparable to that of T. viride when tested on filter paper, carboxymethylcellulose, and cotton; however, it produced 20 to 26 times larger amounts of cellobiase. Both cellulase and beta-glucosidase were obtained in good yield only when cellulose was the carbon source. The production of these enzymes was not repressed by readily assimilated carbon sources in the presence of cellulose. Only traces of cellulase and beta-glucosidase were formed on glucose, fructose, maltose, and cellobiose although good growth was obtained on these substrates. These enzymes were not induced on sophorose, lactose, mannitol, or glycerol and growth was poor on these substrates. Cellobiose octaacetate was a less effective inducer of cellulase and beta-glucosidase than was cellulose.

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Section: Resultsmentioning

confidence: 95%

Cellulase and β-glucosidase production by a basidiomycete species

Shewale¹,

Sadana²

1978

Can. J. Microbiol.

117

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“…This was anticipated from the findings of King (1966) and Rautela (1967); the latter found that solubilization (by enzymatic means) of cellulosic substrates containing large particles is slower than corresponding substrates containing smaller particles, while King states that the rate of enzymatic solubilization is directly proportional to the substrate surface area. .... Ot Generally, the dry-milled substrates produced the higher yields of fungal cellulase for an individual culture-a fact not apparently related to the amount of growth within that culture (see later discussion).…”

Section: Resultsmentioning

confidence: 85%

“…Rautela (1967) was also unable to establish correlations between growth-supporting and inducing capabilities of his cellulosic substrates. Dry ball-milled woodmeal supported growth far better than wet-milled woodmeal.…”

Section: Resultsmentioning

confidence: 97%

The Effect of Subtrate Availability on Cellulolytic Enzyme Production by Selected Wood-Rotting Microorganisms

Greaves¹

1971

Aust. Jnl. Of Bio. Sci.

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Extracellular cellulases have been produced in shake cultures of Polystictus ver8icolor, Fornes anno8U8, Lenzites trabea, Poria monticola, and Bacillus polymyxa, using substrates of cellulose powder and river birch woodmeal. The comparative effect on enzyme production of wet and dry ball milling of the substrates has been examined. Studies were also made to establish the relative amounts of enzyme diffused, bound, or adsorbed during production and the influence of pH on cellulase activity. It was found that wet ball milling reduces the particle size of both materials more rapidly than dry ball milling. There was an overall increase in enzyme released as the particle size of the substrate decreased, but no correlation was established between this phenomenon and growth of the microorganisms. Dry milled substrates of comparable particle size produced higher yields of cellulases than the wet milled substrates, Lenzites trabea being more active in enzyme production than any of the other organisms. Diffusion of cellulases occurred more readily in the brown rot fungi than in the two white rot microorganisms and a higher percentage of enzyme remained bound with the latter group.

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“…Different forms of cellulose vary in the extent of their crystallinity (Rautela & King, 1968), but the finding that filter paper and carboxymethylcellulose, both of which are frequently used for cellulase assays, contain up to 30% hemicelluloses is surprising. Even Avicel, cellulose powder and the freshly prepared straw cellulose contained 10% hemicelluloses.…”

Section: Discussionmentioning

confidence: 99%