Significance of TEAD Family in Diagnosis, Prognosis and Immune Response for Ovarian Serous Carcinoma

Abstract

Purpose: To explore the molecular profiles of transcriptional enhanced associate domain (TEAD) family in ovarian serous carcinoma (OSC). Methods: In this study, we use bioinformatics methods including GEPIA, GE-mini, Oncomine 3.0, Kaplan-Meier plotter, cBioPortal, WebGestalt, TIMER2.0 and DiseaseMeth2.0, and in vitro experimental RT-PCR to assess the expression profiles and prognostic significance of TEAD family in OSC. Results: According to the bioinformatics analysis, TEAD family was abnormally expressed in OSC. In terms of prognosis, Kaplan-Meier plotter indicated that OSC patients with high level of TEAD4 showed poor overall survival (OS), progression-free survival (PFS) and post progression survival (PPS). TEAD family also had significantly diagnostic values for OSC patients. Tumor Immune Estimation Resource (TIMER) algorithm indicated that TEAD family was significantly associated with different types of infiltrating immune cells, including B cells, macrophages, dendritic cells, neutrophils, CD8+ T cells and CD4+ T cells. Gene set enrichment analysis of TEAD family-associated coexpression genes was further explored. In in vitro experiments, the RT-PCR results showed the upregulated TEAD2/4 in OSC tissues and cells (A2780 and TOV112D). Moreover, decreased expression of TEAD2 could induce the ferroptosis through increasing the ROS accumulation. Conclusion: Thus, TEAD family correlated with the diagnosis, prognosis and immune infiltration in OSC. These results could provide comprehensive understanding of TEAD family in the diagnosis and prognosis of OSC patients.

Transcription factors TEAD2 and E2A globally repress acetyl-CoA synthesis to promote tumorigenesis

“…54,55 In addition, it is reported that knockdown of TEAD2 in ovarian serous carcinoma (OSC) induces ferroptosis by increasing ROS. 56 We note that metabolic fitness is reduced in TEAD2 and E2A overexpressing cells upon ARR treatment (Figures 6D-6G). Thus, low acetyl-CoA levels may promote tumorigenesis via hypo-acetylation of pluripotency factors and metabolic enzymes.…”

A comparative study of COVID-19 transcriptional signatures between clinical samples and preclinical cell models in the search for disease master regulators and drug repositioning candidates

“…Regarding the TEAD4, cancer cells overexpressing this transcription factor showed immune system process and immune response pathways such as antigen processing and presentation, natural killer cell mediated cytotoxicity, and T cell receptor signaling pathway differentially activated when compared to controls. The differential expression of the TEAD genes family also showed significant association with different types of immune cells infiltration in the tumor environment ( Ren et al, 2021 ; J. Wang et al, 2021 ).…”

Identification of Downregulated Exosome-Associated Gene ENPP1 as a Novel Lipid Metabolism and Immune-Associated Biomarker for Hepatocellular Carcinoma

“…Next, Venn plots were employed to identify the co-DEGs among the exosome-associated gene dataset (Supplementary Table 1 ) [ 24 ] and the above-mentioned GEO datasets. After then, Xiantao Xueshu [ 25 ], TNMplot [ 26 ], and UALCAN [ 27 ] were used to confirm the downregulated expression levels of ENPP1 in LIHC tissues.…”