Significance of solute specificity, expression, and gating mechanism of tonoplast intrinsic protein during development and stress response in plants

Sudhakaran, Sreeja; Thakral, Vandana; Padalkar, Gunashri; Rajora, Nitika; Dhiman, Pallavi; Raturi, Gaurav; Sharma, Yogesh; Tripathi, Durgesh Kumar; Deshmukh, Rupesh; Sharma, Tilak Raj; Sonah, Humira

doi:10.1111/ppl.13386

Cited by 23 publications

(13 citation statements)

References 123 publications

(204 reference statements)

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“…TIPs are involved in the maintenance of water permeability, cell turgor regulation, and solute transport. PIPs and TIPs have been found to play an important role during salt, drought, and cold stress (Afzal et al 2016;Sudhakaran et al 2021).…”

Section: Discussionmentioning

confidence: 99%

Identification of aquaporins and deciphering their role under salinity stress in pomegranate (Punica granatum)

Kumawat

Aggarwal

Rana

et al. 2021

J. Plant Biochem. Biotechnol.

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Aquaporins (AQPs), pore-forming proteins, are known to involve in the transport of water and many other small solutes, and play a diverse role in physiological processes. In this study, 38 AQPs were identified from pomegranate (Punica granatum) genome based on the phylogenetic distribution and homology with known AQPs from Arabidopsis thaliana and Oryza sativa. The pomegranate AQPs were further classified into five subgroup representing 12 plasma membrane intrinsic proteins (PIPs), 8 Nod26-like intrinsic proteins (NIPs), 14 tonoplast intrinsic proteins (TIPs), three small and basic intrinsic proteins (SIPs) and one uncategorized intrinsic protein (XIPs). Extensive computational analysis was performed to understand the pore morphology, exon-intron structure, and subcellular localization of the AQPs. The identified AQPs were further confirmed for the conserved NPA motifs, ar/R selectivity filters, and Froger's residues. Analysis of the available transcriptomic data revealed tissue specific expression of different aquaporins, for instance, under salinity stress PgNIP6-1 showed increased expression in root. The co-expression has been observed among PgTIP3-1, PgTIP1-4, PgTIP1-8, PgTIP1-1, PgSIP1-1, PgPIP2-4, PgPIP1-3, and PgPIP1-2. Moreover, PgTIP3-1 showed a negative correlation with remaining seven AQPs. Further, the expression analysis of PgNIP6-1, PgTIP3-1 and PgNIP3-1 using quantitative real-time PCR showed increased transcript level of these genes under salinity stress suggesting their key role in stress related mechanism. The information presented here will provide insights into AQPs role in development and stress tolerance in plants.

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Section: Discussionmentioning

confidence: 99%

Identification of aquaporins and deciphering their role under salinity stress in pomegranate (Punica granatum)

Kumawat

Aggarwal

Rana

et al. 2021

J. Plant Biochem. Biotechnol.

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“…TIPs are multifunctional channel proteins that not only transport water but also many other small solutes, such as NH 3 , urea, H 2 O 2 , and glycerol [7]. It has been found that TIPs have a strong water transport capacity, which can be up to 100-fold than that of PIP in Xenopus oocytes [6].…”

Section: Introductionmentioning

confidence: 99%

Overexpression of a Cotton Aquaporin Gene GhTIP1;1-like Confers Cold Tolerance in Transgenic Arabidopsis

Cheng

Wang

Zhang

et al. 2022

IJMS

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Cold stress can significantly affect the development, yield, and quality of crops and restrict the geographical distribution and growing seasons of plants. Aquaporins are the main channels for water transport in plant cells. Abiotic stresses such as cold and drought dehydrate cells by changing the water potential. In this study, we cloned a gene GhTIP1;1-like encodes tonoplast aquaporin from the transcriptome database of cotton seedlings after cold stress. Expression analysis showed that GhTIP1;1-like not only responds to cold stress but was also induced by heat, drought and salt stress. Subcellular localization showed that the protein was anchored to the vacuole membrane. Promoter deletion analysis revealed that a MYC motif within the promoter region of GhTIP1;1-like were the core cis-elements in response to low temperature. Virus-induced gene silencing (VIGS) and histochemical staining indicate that GhTIP1;1-like plays a positive role in plant cold tolerance. Overexpression of GhTIP1;1-like in Arabidopsis delayed the senescence process and enhanced the cold tolerance of transgenic plants. Compared with the wild type, the soluble protein concentration and peroxidase activity of the transgenic lines under cold stress were higher, while the malondialdehyde content was lower. In addition, the expression levels of cold-responsive genes were significantly increased in transgenic plants under cold stress. Our results indicate that GhTIP1;1-like could respond to different abiotic stresses and be positively involved in regulating the cold tolerance of cotton.

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“…So far, five subclasses of AQPs have been recognized from sequence comparisons and subcellular localization in plants, including: tonoplast intrinsic proteins (TIPs), plasma membrane intrinsic proteins (PIPs), nodulin 26-like intrinsic proteins (NIPs), small and basic intrinsic proteins (SIPs) and X intrinsic proteins (XIPs) [ 7 , 10 , 12 , 15 ]. Among them, the TIPs are predominately localized within the tonoplast membrane of the vacuoles of most plant cells [ 16 , 17 ]. The PIP proteins can be divided into PIP1 and PIP2, two distinct subcategories based on sequence comparisons and transport activity [ 10 , 12 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

Identification and Characterization of Salt- and Drought-Responsive AQP Family Genes in Medicagosativa L.

Luo

et al. 2022

IJMS

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Aquaporins (AQP) are distributed ubiquitously in plants, and they play important roles in multiple aspects of plant growth and development, as well as in plant resistance to various environmental stresses. In this study, 43 MsAQP genes were identified in the forage crop Medicago sativa. All the MsAQP proteins were clustered into four subfamilies based on sequence similarity and phylogenetic relationship, including 17 TIPs, 14 NIPs, 9 PIPs and 3 SIPs. Analyses of gene structure and conserved domains indicated that the majority of the deduced MsAQP proteins contained the signature transmembrane domains and the NPA motifs. Analyses on cis-acting elements in the promoter region of MsAQP genes revealed the presence of multiple and diverse stress-responsive and hormone-responsive cis-acting elements. In addition, by analyzing the available and comprehensive gene expression data of M. truncatula, we screened ten representative MtAQP genes that were responsive to NaCl or drought stress. By analyzing the sequence similarity and phylogenetic relationship, we finally identified the corresponding ten salt- or drought-responsive AQP genes in M. sativa, including three MsTIPs, three MsPIPs and four MsNIPs. The qPCRs showed that the relative expression levels of these ten selected MsAQP genes responded differently to NaCl or drought treatment in M. sativa. Gene expression patterns showed that most MsAQP genes were preferentially expressed in roots or in leaves, which may reflect their tissue-specific functions associated with development. Our results lay an important foundation for the future characterization of the functions of MsAQP genes, and provide candidate genes for stress resistance improvement through genetic breeding in M. sativa.

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Significance of solute specificity, expression, and gating mechanism of tonoplast intrinsic protein during development and stress response in plants

Cited by 23 publications

References 123 publications

Identification of aquaporins and deciphering their role under salinity stress in pomegranate (Punica granatum)

Identification of aquaporins and deciphering their role under salinity stress in pomegranate (Punica granatum)

Overexpression of a Cotton Aquaporin Gene GhTIP1;1-like Confers Cold Tolerance in Transgenic Arabidopsis

Identification and Characterization of Salt- and Drought-Responsive AQP Family Genes in Medicagosativa L.

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