Significance of Serum Lactate Dehydrogenase and its Isoenzymes During Post-Burn Follow-Up

Abstract

Kratak sadr`aj: Cilj studije je da se odredi uloga izo enzima laktat dehidrogenaze (LDH) u termi~kim opeko tinama. Analizirano je ukupno 18 pacijenata oba pola sa ukupnom povr{inom opekotina 20 -50% koji su primljeni na Odeljenje za opekotine Medicinskog koled`a i bolnice D`avaharlal Nehru. Pacijenti su podvrgnuti op{tim i sistemskim ispitivanjima. Za kvantifikaciju LDH kori{}eni su serumi uzeti posle 1, 2, 5 i 10 dana pra}enja stanja paci jenata sa opekotinama. Profili PAGE pokazali su zna~ajne razlike u nivoima izoenzima LDH kod svih pacijenata sa opekotinama (P=0,05). Softverskom analizom gel-skenova otkriveno je prisustvo pet nizova izoenzima, od kojih su LDH-1 i -2 dali najmanji doprinos. Tokom pra}enja stanja uo~eno je da se rangiranje izoenzima LDH pribli`ava kontrol nim vrednostima 2. dana kod pacijenata sa ukup nom povr{inom opekotina od 20%, dok se u preostalim slu~ajevima isto doga|a 5. dana. Trodimenzionalni densi togrami MERA~ GUSTINE pokazali su visoku aktivnost LDH kod 50% pacijenata sa opekotinama ~ak i 10. dana, me|utim, relativno rangiranje tih izoenzima bilo je sli~no kontrol nim vrednostima (LDH-4>-5>-3>-1>-2). Mi{lje nja smo da se visoka aktivnost enzima LDH javlja usled kompleksa enzim-imunoglobulin-G (LDH-IgG), ali za~udo nismo uo~ili taj kompleks kod 50% pacijenata sa opekotinama u bilo kom tre nutku. Stoga, verujemo da izoenzimi LDH u~estvuju u pato fiziologiji bolesti i mogu biti korisni za utvr|ivanje nevidljivih o{te}enja tkiva. {tavi{e, visoka aktivnost LDH kod 50% pacijenata sa opekotinama javlja se usled nekog drugog mehanizma a ne usled vezivanja LDH za IgG. Summary: The present study aims to evaluate the role of lactate dehydrogenase (LDH) isoenzymes in thermal burns. A total of 18 patients of both genders with 20 to 50% total burn surface area (TBSA), admitted to the Burn Ward of JN Medical College and Hospital was assessed. These patients were subjected to general and systemic examinations. The sera collected at day 1, 2, 5 and 10 during follow-up of burn patients were used for LDH quantitation. PAGE profiles showed significant differences in the levels of LDH iso enzymes in all the burn subjects (P=0.05). Software analysis of gel-scans showed the presence of five isoenzyme bands of which LDH-1 and -2 are the least contributors. During follow-up, it was observed that the ranking of LDH isoenzymes approaches control values at day 2 in 20% TBSA patients, while in the remaining cases it occurs at day 5. 3D-densi tograms indicated high activity of LDH in 50% of TBSA patients even at day 10; however, the relative ranking of these isoenzymes was similar to control values (LDH-4>-5>-3>-1>-2). We were of the opinion that the high activity of LDH enzyme is due to the enzyme-immunoglobulin-G (LDH-IgG) complex, but surprisingly we did not observe this complex in 50% of burn patients at any of the durations. Therefore, it is suggested that LDH isoenzymes play a role in the patho physiology of the disease and can be an asset to ascertain the invisible tissue damage. Moreover, the high activ...

Wound Healing Composite Materials of Bacterial Cellulose and Zinc Oxide Nanoparticles with Immobilized Betulin Diphosphate

“…Zinc oxide nanoparticles can affect indirect antioxidant properties and be potential regulators of redox imbalance during the burn disease due to tissue hypoxia in the wound. ZnO NPs’ influence on oxidative stress, increases the generation of reactive oxygen species (ROS) but increases antioxidant enzyme defense and regulates energy metabolism [ 18 , 19 , 20 , 21 , 22 ].…”