Significance of Serum L-fucose Glycoprotein as Cancer Biomarker in Head and Neck Malignancies without Distant Metastasis

Shetty, Rathan; Bhandary, Satheesh Kumar; Kali, Arunava

doi:10.7860/jcdr/2013/6681.3765

Cited by 7 publications

(8 citation statements)

References 15 publications

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“…In Table 3, we summarize findings from studies comparing serum fucose and fucosylated glycan profiles from healthy individuals vs. breast, oral/head and neck, HCC, ovarian, CRC, pancreatic, lung and prostate cancer patients. Several of these studies suggest diagnostic potential for serum L-fucose levels, which have been found to be elevated in cancer patient sera in breast [53,54,55], oral/head and neck [56,57,58,59,60,61], HCC [62,63,64,65], CRC [66,67], and ovarian [55,68] cancer patients compared with healthy individuals. Specific fucosylated glycoconjugates have been found to also have potential diagnostic utility.…”

Section: Serum Fucose and Fucosylated Glycoconjugatesmentioning

confidence: 99%

The Diverse Contributions of Fucose Linkages in Cancer

Keeley

Yang

Lau

2019

Cancers

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Fucosylation is a post-translational modification of glycans, proteins, and lipids that is responsible for many biological processes. Fucose conjugation via α(1,2), α(1,3), α(1,4), α(1,6), and O’- linkages to glycans, and variations in fucosylation linkages, has important implications for cancer biology. This review focuses on the roles that fucosylation plays in cancer, specifically through modulation of cell surface proteins and signaling pathways. How L-fucose and serum fucosylation patterns might be used for future clinical diagnostic, prognostic, and therapeutic approaches will be discussed.

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Section: Serum Fucose and Fucosylated Glycoconjugatesmentioning

confidence: 99%

The Diverse Contributions of Fucose Linkages in Cancer

Keeley

Yang

Lau

2019

Cancers

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“…Although metastasis accounts for about 90% of cancer-related mortality, the molecular mechanism of tumor metastasis is still poorly understood [1–4]. Migration, invasion, and adhesion of tumor cells are important steps in the process of tumor metastasis [5–7]. In order to investigate whether Orai1 can be involved in regulating the metastasis of osteosarcoma cells, we used Orai1 siRNAs to successfully silence the expression of Orai1 in MG-63 osteosarcoma cells.…”

Section: Resultsmentioning

confidence: 99%

“…Tumor cell metastasis includes invasion into surrounding tissues, infiltration into circulation, migration, and survival in distant organs. Although metastasis accounts for about 90% of cancer-related mortality, the molecular mechanism of tumor metastasis is still poorly understood [5–7]. Migration, invasion, and adhesion of tumor cells run through the whole process of tumor metastasis.…”

Section: Discussionmentioning

confidence: 99%

“…Although metastasis accounts for about 90% of cancer-related mortality, the molecular mechanism of tumor metastasis is still poorly understood. Migration, invasion, and adhesion of tumor cells are crucial steps in the process of tumor metastasis [5–7]. Clarifying the regulatory mechanism of cell migration, invasion, and adhesion, and taking comparable therapeutic measures to inhibit metastasis could potentially be new ideas for the treatment of osteosarcoma.…”

Section: Introductionmentioning

confidence: 99%

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Orai1 Promotes Osteosarcoma Metastasis by Activating the Ras-Rac1-WAVE2 Signaling Pathway

et al. 2019

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BackgroundThe purpose of this study was to investigate whether Orai1 plays a role in the metastasis of osteosarcoma.Material/MethodsThe expression of Orai1 was silenced by small interfering RNAs against Orai1 (Orai1 siRNA) in osteosarcoma MG-63 cells. Various experiments were carried out to detect the changes in migration, invasion, and adhesion ability of these osteosarcoma cells. Furthermore, the activity of Rac1, Wave2, and Ras was detected using Western blot analysis. Moreover, the Rac1 and Ras inhibitors were used to confirm whether the Ras-Rac1-WAVE2 signaling pathway was involved in osteosarcoma metastasis promoted by Orai1.ResultsWe found that the migration, invasion, and adhesion ability of MG-63 cells were significantly reduced after silencing Orai1 expression (p<0.05). Moreover, the activity of the Rac1-WAVE2 signaling pathway was significantly inhibited after silencing of Orai1 expression (p<0.05). After the Rac1 inhibitor was added, Orai1 siRNA could not further inhibit migration, invasion, and adhesion of the osteosarcoma cells. Further experiments showed that Ras activity was significantly inhibited after silencing Orai1 expression (p<0.05). Moreover, Orai1 siRNA did not further inhibit the activity of the Rac1-WAVE2 signaling pathway nor did it further inhibit the migration, invasion, and adhesion ability of osteosarcoma cells following the addition of Ras inhibitors.ConclusionsOrai1 activates the Ras-Rac1-WAVE2 signaling pathway to promote metastasis of osteosarcoma. Abnormal expression or function of Orai1 may be an important cause of osteosarcoma metastasis.

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“…However, there was a negative correlation between serum fucose levels and histopathological tumor differentiation in our study as well as in the literatures. [ 15 ]…”

Section: Discussionmentioning

confidence: 99%