Significance of Platelet-Derived Microparticles in Uremia

Nomura, Shosaku; Shouzu, Akira; Nishikawa, Mitsushige; Kokawa, Terutoshi; Yasunaga, Kōjirō

doi:10.1159/000187269

Cited by 27 publications

(19 citation statements)

References 9 publications

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“…Therefore, increased PMP may be a cause of hypercoagulability [12]. Increase in PMP is not specific to diabetes mellitus, and is also observed in uremia and cerebral infarction [17, 20]. In addition, increase in PMP level was observed in disease states in which activated platelets have been detected in vivo [17, 18, 19, 20].…”

Section: Discussionmentioning

confidence: 99%

“…Increase in PMP is not specific to diabetes mellitus, and is also observed in uremia and cerebral infarction [17, 20]. In addition, increase in PMP level was observed in disease states in which activated platelets have been detected in vivo [17, 18, 19, 20]. Nomura et al [18]reported that numbers of PMP were significantly greater in diabetics with high low-density lipoprotein levels than in these with low low-density lipoprotein levels, suggesting that PMP may participate in the development or progression of atherosclerosis in patients with diabetes mellitus.…”

Section: Discussionmentioning

confidence: 99%

“…Platelet-derived microparticles (PMP) are produced by platelet activation or by physical stimulation under various conditions [14, 15, 16, 17, 18, 19, 20]. PMP contain inner granules and membranous microvesicles released from activated platelets and membranous fragments produced by mechanical destruction [14], and have coagulative activity [15, 16].…”

Section: Introductionmentioning

confidence: 99%

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Significance of Platelet-Derived Microparticles and Activated Platelets in Diabetic Nephropathy

Omoto¹,

Nomura²,

Shouzu³

et al. 1999

Nephron

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We measured levels of platelet-derived microparticles (PMP), which have coagulative activity and are produced by platelet activation or physical stimulation, and CD62P/CD63-positive platelets in patients with diabetes mellitus to determine their clinical significance and effects on complications of diabetes including diabetic nephropathy. We also compared these levels before and after administration of the antiplatelet drug cilostazol. Plasma PMP and CD62P/CD63-positive platelet levels were significantly higher in patients with diabetes mellitus than normal controls. CD62P-positive platelet levels were significantly higher in patients with nephropathy than in patients without complications. After administration of cilostazol, PMP and CD62P/CD63-positive platelet levels were significantly decreased. The increases in platelet activity and its related procoagulant activity appear to account in part for the hypercoagulability observed in diabetes mellitus. Our findings suggest that activated platelets might play a role in the development of diabetic nephropathy. Furthermore, antiplatelet therapy with cilostazol for diabetic patients may be useful as antithrombin therapy including antiplatelet therapy, since it suppresses the production of intrinsic coagulants produced by platelet activation.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Significance of Platelet-Derived Microparticles and Activated Platelets in Diabetic Nephropathy

Omoto¹,

Nomura²,

Shouzu³

et al. 1999

Nephron

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“…Monitoring plasma levels of PPLs may provide a direct measure of the degree of activation of primary haemostasis. Elevated numbers of PMPs in the circulation have been reported in a number of pathological conditions, such as heparin-induced thrombocytopenia [9], uraemia [10], disseminated intravascular coagulation [11], myocardial infarctions [12], unstable angina [13] and diabetes [14]. Thus, they play an important physiological role, and measurement of PMPs has, therefore, received increased attention in recent years as a diagnostic aid and investigative tool.…”

Section: Introductionmentioning

confidence: 99%

Clinical evaluation of a new functional test for detection of plasma procoagulant phospholipids

Dreden

Rousseau

Fontaine

et al. 2009

Blood Coagulation &Amp; Fibrinolysis

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The objective of this study was to validate a simple factor Xa-based clotting test developed to monitor procoagulant phospholipids (PPLs) and platelet-derived microparticles (PMPs). This assay is easily automated, giving it a major advantage over the more laborious and expensive flow cytometry, electron microscopy and ELISA techniques in general usage at present. The intra-assay and inter-assay variation coefficients were less than 5% at both low and high levels of PPLs. The test is not affected by other clotting factors is assured by the use of a phospholipid-free animal plasma, which provides excess factor V, fibrinogen and prothrombin. This test was evaluated in apparently healthy volunteers and in selected patient groups associated with increased levels of PMPs in the circulation (diabetes mellitus, sickle cell disease, thyroid cancer and patients with multiple trauma). The study showed that XaCT has a high discriminating power for PPLs and that the patient groups have significantly highly increased PPLs activities when compared with healthy volunteers. Although of a preliminary nature, the test has shown that it has the sensitivity for discriminating severity of disease, as it could detect patients in sickle cell crisis and differentiate between type 1 and 2 diabetes. In conclusion, the combination of reliability, reproducibility and easy performance makes the XaCT assay a simple test to screen for PPLs in plasma samples.

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“…The functional significance o f these plateletderived microparticles is o f considerable in terest because they are rich in membrane receptors for coagulation factor Va and pro vide a catalytic surface for the prothrombinase reaction [2], Microparticles have been detected in various clinical situations associ ated with platelet activation, including im mune thrombocytopenic purpura [3][4][5], thrombotic diseases [6,7] and cardiopulmo nary bypass [8]. These observations suggest that microparticles may play a role in modu lating hemostasis and thrombosis, but the mechanism o f their production remains un resolved.…”

Section: Introductionmentioning

confidence: 99%

Participation of α<sub>IIb</sub>β<sub>3</sub> in Platelet Microparticle Generation by Collagen plus Thrombin

Nomura¹,

Komiyama

Matsuura

et al. 1996

Pathophysiol Haemos Thromb

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We investigated the role of α_IIbβ₃ in microparticle generation by normal and thrombasthenic platelets stimulated with collagen plus thrombin. Microparticle generation by normal platelets was scarcely inhibited by monoclonal antibodies for glyco-protein lb and glycoprotein IX. Although one monoclonal anti-α α_IIbβ₃ antibody (NNKY1-32) partly inhibited microparticle generation, 3 other monoclonal anti-α α_IIbβ₃ antibodies had little effect. However, the combination of 4 monoclonal anti- α_IIbβ₃ antibodies or treatment with a polyclonal anti- α_IIbβ₃ antibody significantly inhibited microparticle generation (p < 0.05). Microparticle generation by thrombasthenic platelets also occurred after stimulation with collagen plus thrombin, although at a significantly lower level compared with normal platelets. Monoclonal antibodies for resting α_IIbβ₃, P-selectin, activated α_IIbβ₃ and β₂-glycoprotein I bound to microparticles from healthy platelets. In contrast, only a monoclonal antibody for β₂-glycoprotein I bound to thrombasthenic microparticles. These results suggest that microparticle generation by collagen plus thrombin occurs via two different mechanisms which are dependent and independent of α_IIbβ₃, respectively. The α_IIbβ₃-dependent mechanism appears to require activation of α_IIbβ₃·

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Significance of Platelet-Derived Microparticles in Uremia

Cited by 27 publications

References 9 publications

Significance of Platelet-Derived Microparticles and Activated Platelets in Diabetic Nephropathy

Significance of Platelet-Derived Microparticles and Activated Platelets in Diabetic Nephropathy

Clinical evaluation of a new functional test for detection of plasma procoagulant phospholipids

Participation of α<sub>IIb</sub>β<sub>3</sub> in Platelet Microparticle Generation by Collagen plus Thrombin

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