Significance of Mitochondrial Reactive Oxygen Species in the Generation of Oxidative Stress in Spermatozoa

Koppers, Adam J.; Iuliis, Geoffry N. De; Finnie, Jane M.; McLaughlin, Eileen A.; Aitken, R. John

doi:10.1210/jc.2007-2616

Cited by 549 publications

(464 citation statements)

References 37 publications

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“…Mitochondrial ROS anions have been shown to be generated from MCI while hydrogen peroxide from mitochondrial complex III in human sperm 8. Based on the above findings, in this study, the diaphorase‐type activity of sperm MCI, independent of ubiquinone, was detected in sperm homogenates using the Complex I Enzyme Activity Microplate Assay Kit (#ab190721; Abcam).…”

Section: Methodsmentioning

confidence: 90%

“…The finding of increased mROS in sperm from infertile men is consistent with the report of Koppers et al . 8 in sperm from fertile men after treatment with rotenone (an inhibitor of electron transfer in MCI). Furthermore, a significantly positive correlation between high MMP and the sperm motility (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…These results agree with the recent report of Koppers et al . 8, who showed that mROS is generated by defective human spermatozoa and is negatively correlated with motility. Moreover, the induction of ROS on the matrix side of the inner mitochondrial membrane in complex I (MCI) by disruption of mitochondrial electron transport flow resulted in peroxidative damage and a loss of movement in human spermatozoa 8.…”

Section: Discussionmentioning

confidence: 99%

“…8, who showed that mROS is generated by defective human spermatozoa and is negatively correlated with motility. Moreover, the induction of ROS on the matrix side of the inner mitochondrial membrane in complex I (MCI) by disruption of mitochondrial electron transport flow resulted in peroxidative damage and a loss of movement in human spermatozoa 8. Taken together, in this study, we further demonstrate that sperm from infertile men are capable of increased production of mROS (superoxide), resulting in lower high MMP, higher percentages of cells with lipid peroxidation and a loss of movement (Figs 1, 2, 3).…”

Section: Discussionmentioning

confidence: 99%

“…Once in this location, the probe is oxidized by superoxide anion, generating a red fluorescence 8. For the assay, the sperm suspensions (10 × 10 6 cells/ml) were incubated for 15 min.…”

Section: Methodsmentioning

confidence: 99%

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Prohibitin involvement in the generation of mitochondrial superoxide at complex I in human sperm

Chai

Chen

Shi

et al. 2016

J Cellular Molecular Medi

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Prohibitin (PHB), a major mitochondrial membrane protein, has been shown earlier in our laboratoryto regulate sperm motility via an alteration in mitochondrial membrane potential (MMP) in infertile men with poor sperm quality. To test if PHB expression is associated with sperm mitochondrial superoxide (mROS) levels, here we examined sperm mROS levels, high MMP and lipid peroxidation in infertile men with poor sperm motility (asthenospermia, A) and/or low sperm concentrations (oligoasthenospermia, OA). The diaphorase‐type activity of sperm mitochondrial complex I (MCI) and PHB expression were also determined. We demonstrate that mROS and lipid peroxidation levels are significantly higher in sperm from A and OA subjects than in normospermic subjects, whereas high MMP and PHB expression are significantly lower. A positive correlation between mROS and lipid peroxidation and a negative correlation of mROS with PHB expression, high MMP, and sperm motility were found in these subjects. The finding of similar diaphorase‐type activity levels of sperm MCI in the three groups studied suggests that the catalytic subunits of MCI in the matrix arm may produce mROS on its own. There may be a dysfunction of electron transport at MCI associated with decreased expression of PHB in sperm with poor quality. We conclude that mROS level is increased and associated with decreased PHB expression, and it may regulate sperm motility via increases in low MMP and lipid peroxidation. This is the first report on the involvement of PHB in human sperm motility loss associated with increased generation of mROS at MCI.

show abstract

Section: Methodsmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…Once in this location, the probe is oxidized by superoxide anion, generating a red fluorescence 8. For the assay, the sperm suspensions (10 × 10 6 cells/ml) were incubated for 15 min.…”

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Prohibitin involvement in the generation of mitochondrial superoxide at complex I in human sperm

Chai

Chen

Shi

et al. 2016

J Cellular Molecular Medi

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Mammalian Sperm Mitochondrial Function as Affected by Environmental Toxicants, Substances of Abuse, and Other Chemical Compounds

Amaral

Tavares

Escada-Rebelo

et al. 2018

Mitochondrial Dysfunction Caused by Drugs and Environmental Toxicants

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Are old males still good males and can females tell the difference?

Johnson

Gemmell

2012

BioEssays

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Sperm function generally declines with male age. Paradoxically, females of many species still choose to mate with old males rather than young males. Females choosing old mates may suffer reduced fertilization rates and an increased incidence of birth defects in offspring, lowering fitness which may in turn lead to conflict between the sexes. This apparent paradox has generated much interest from theorists, but whether this paradox presents in nature remains equivocal. Empirical studies have found mixed support for both a decline in fertility with male age and age-based female mate preference. Here, we examine recent evidence for this paradox, identify confounding variables, highlight areas that deserve further investigation, and suggest avenues for future research.

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Significance of Mitochondrial Reactive Oxygen Species in the Generation of Oxidative Stress in Spermatozoa

Abstract: We conclude that the sperm mitochondria make a significant contribution to the oxidative stress experienced by defective human spermatozoa.

Cited by 549 publications

References 37 publications

Prohibitin involvement in the generation of mitochondrial superoxide at complex I in human sperm

Prohibitin involvement in the generation of mitochondrial superoxide at complex I in human sperm

Mammalian Sperm Mitochondrial Function as Affected by Environmental Toxicants, Substances of Abuse, and Other Chemical Compounds

Are old males still good males and can females tell the difference?

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