Significance of dihydropyrimidine dehydrogenase and thymidylate synthase mRNA expressions in hepatocellular carcinoma

Abstract

The low DPD mRNA expression is a significant poor prognostic factor. after curative resection of HCC.

Role of thymidylate synthase and dihydropyrimidine dehydrogenase mRNA expressions in gallbladder carcinoma

“…Alternatively, differences in the methods of obtaining tumor tissue specimens might explain such discrepant results. In this study, we utilized laser microdissection according to the Danenberg tumor profile method [17].…”

“…Danenberg tumor profile method and PCR quantification of mRNA expression Intratumoral TS and DPD mRNA expressions were examined using the Danenberg tumor profile method, as described previously [17]. This method consists of the laser microdissection of tumor cells on FFPE specimens, RNA extraction, cDNA synthesis, and quantification of mRNA expression by real-time PCR.…”

“…It has been reported that TS activity was generally lower in hepatocellular carcinoma (HCC) [13], and that downregulation of DPD gene expressions may enhance the negative prognostic effect in colorectal tumors [14] and ovarian cancer [15]. We reported recently that negative DPD expression was significantly associated with increased tumor cell proliferation and poorer prognosis in intrahepatic cholangiocarcinoma [16], and that the low expression of DPD mRNA was a poor prognostic factor in HCC [17]. However, there is no report examining the clinicopathological significance of these gene expressions in GBC.…”

Role of thymidylate synthase and dihydropyrimidine dehydrogenase mRNA in intrahepatic cholangiocarcinoma

“…Thus, DPD expression seems to be associated with malignant behavior including prognosis, although the mechanism of expression in malignant tumors remains unclear. As a possible mechanism, we assumed that decreased DPD expression induced activation of DNA synthesis, and recently confirmed the correlation between DPD mRNA expression and Ki-67 labeling index in HCC [34]. In HCC patients with DPD mRNA low expression, the proliferation index of the tumor was significantly higher and prognosis was poorer than in those with DPD mRNA high expression.…”

Elevated levels of mRNAs encoding dihydropyrimidine dehydrogenase and thymidylate synthase are associated with improved survival of patients with hepatocellular carcinoma treated with S-1

“…Using qPCR, Nii et al (19) measured TYMS and DPYD mRNA levels in 74 patients with HCC and demonstrated that the OS time of patients with high levels of DPYD mRNA was significantly longer compared with patients with low levels of DPYD mRNA. These findings are in complete agreement with those of the present study.…”