Significance of AgNOR counts for distinguishing carcinoma from adenoma and hyperplasia in parathyroid gland

Kanematsu, Emiko; Matsui, Hiroe; Deguchi, Takashi; Yamamoto, Osami; Korematsu, Motoko; Kobayashi, Akira; Nezasa, Shinichi; Yamamoto, Naoki; Takeuchi, Toshimi; Tanaka, Takuji; Kawada, Yukimichi

doi:10.1016/s0046-8177(97)90030-7

Cited by 13 publications

(15 citation statements)

References 25 publications

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“…26 Many authors have not observed differences in the numbers of AgNORs in benign neoplastic and hyperplastic cells. 10,14 However, in this study, a statistically significant difference was detected between the number of AgNORs in the nuclei of follicular cells in the thyroids from group A and those in glands from groups B and C ( Table 1). The thyroids from group A showed small AgNORs scattered throughout the nucleus.…”

Section: Discussioncontrasting

confidence: 49%

Histomorphometry and Quantification of Nucleolar Organizer Regions in Bovine Thyroid Containing Methylthiouracil Residues

et al. 1999

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Abstract. In order to study the morphology and morphometry and to characterize and quantify the nucleolar organizer regions (NORs) of bovine thyroids containing methylthiouracil (MTU) residues, five animals were orally treated with a suspension of MTU (5 g/animal/day) for 20 days (group A). This treatment protocol was interrupted 5 days before the the animals were slaughtered. Six animals receiving placebos composed group B. A third group (group C) was composed of normal thyroids obtained from a slaughterhouse. All glands were previously assessed for detection of antithyroid residues by chromatography, and only those glands from MTUtreated animals were positive. Follicles of glands from group A showed wide variation in size and shape. There was a predominance of small follicles covered by multiple layers of columnar cells, sometimes forming papillary projections into the lumen, characterizing severe interfollicular and intrafollicular adenomatosis. Many follicles had vacuolated cells with nuclei showing karyolysis or pyknosis and reduced amounts of a low-density and very excavated colloid. They also showed higher follicular epithelia and larger proportions of their structural components when compared with glands of groups B and C. In the thyroids from group A, the argyrophilic nucleolar organizer region-associated proteins (AgNORs) were greater in number, with small ones scattered all over the nucleus. Although the size of AgNORs in thyroids from groups B and C was variable, these AgNORs were fewer and larger than were those in glands from group A. In conclusion, the MTU induces proliferation and regressive changes in follicular cells, and the AgNOR technique is efficient to distinguish different degrees of thyroid hyperplasia.

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Section: Discussioncontrasting

confidence: 49%

Histomorphometry and Quantification of Nucleolar Organizer Regions in Bovine Thyroid Containing Methylthiouracil Residues

et al. 1999

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“…Few studies consider the usefulness of labelling indices of PCNA and Ki-67 positivity as markers of the biological behaviour of PC (Abbona et al 1995, Kameyama et al 2000. Kanematsu et al (1997) Endocrine-Related Cancer (2004) 11 563-569 25 parathyroid lesions of which three were PC, found that the AgNOR numbers were significantly higher in PC than in PA. The authors concluded that AgNOR may be useful as an adjunct in discriminating PC from benign parathyroid disease.…”

Section: Discussionmentioning

confidence: 99%

“…The numbers of AgNOR, visualized as distinct black dots, were counted by means of an image-analysis system, using a graphic monitor (Crocker & Nar 1987). AgNOR counting was performed on 100 randomly selected nuclei of parathyroid cells, using a Â100 oil-immersion objective lens (Kanematsu et al 1997). The mean number of AgNORs per nucleus in each specimen was recorded in a database.…”

Section: Nor Silver Staining and Agnor Measurementmentioning

confidence: 99%

Relationship of AgNOR counts and nuclear DNA content to survival in patients with parathyroid carcinoma

Lumachi¹,

Ermani²,

Marino³

et al. 2004

Endocr Relat Cancer

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The aim of this study was to evaluate the usefulness of DNA flow cytometry to determine tumor nuclear DNA index (DI), and nucleolar organizer region protein counts visualized by the argyrophil (AgNOR) technique, in confirming diagnosis and predicting clinical outcome of patients with parathyroid carcinoma (PC). We reviewed paraffin-embedded tissue sections, from 15 patients (median age 63 years, range 30-68 years) with PC who died of the disease, which were randomly compared with tissue sections from 15 age-and sex-matched patients with parathyroid adenoma (PA). The proliferative activity in parathyroid tumours as detected by DI and AgNOR counts was evaluated in all specimens. Both DI (1.37 AE 0.33 vs 1.0 AE 0.1) and AgNOR (3.01 AE 0.31 vs 1.54 AE 0.35) counts were higher ðP < 0:001Þ (Student's t-test) in patients with PC than in those with PA. Diploid (DI¼1), aneuploid (DI > 1) and hypoploid (DI < 1) neoplasms were found in 11 (PC ¼ 4, PA ¼ 7), 14 (PC ¼ 11, PA ¼ 3) and five (PC ¼ 0, PA ¼ 5) patients respectively. The average postoperative survival in patients with PC was 46.9 AE 37.4 months (range 21-146 months). The survivals of patients with aneuploid ðn ¼ 11Þ and diploid ðn ¼ 4Þ PC were 74.0 AE 58.1 and 34.1 AE 18.4 months (P=0.21) respectively. There was a significant relationship between DI and AgNOR counts (R=0.69, P < 0:01), but no correlation was found between survival and both DI (Rho ¼ 0.17, P ¼ 0:55) and AgNOR counts (Rho ¼ 0.26, P ¼ 0:35). Moreover, there was no correlation ðP ¼ NS) between the main preoperative biochemical parameters and survival. In conclusion, DI and AgNOR are useful in confirming the diagnosis of PC, but they are of little value in predicting the clinical outcome of patients with PC.

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“…Indeed, AgNOR analysis may be helpful in distinguishing PC from adenoma. 10,22,30,31 Tuccari et al 10 showed that NORA values assist in distinguishing hyperplastic, adenomatous and cancerous parathyroid lesions because the corresponding mean±SE NORA values were 2.895±0.171, 3.638±0.125 and 4.701±0. 179 μm.…”

Section: Casementioning

confidence: 99%

Parathyroid carcinoma as a challenging diagnosis: Report of three cases

Campennì¹,

Ruggeri²,

Sindoni³

et al. 2012

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Parathyroid carcinoma (Pc) is a rare malignancy with an indolent but progressive course. This rare tumour is often difficult to diagnose preoperatively, thus limiting the efficacy of surgery. As long-term survival is largely dependent on the extent of the primary surgical resection, it is of great importance to consider Pc in the differential diagnosis of hyperparathyroidism. We herein report three Pc patients with different clinical histories who were followed up at our hospital for over a 5-year period, emphasizing the variability in clinical presentation of this rare tumour. Moreover, NOrA (Nucleolar Organizer regions, a standardised silver-stain, marker of proliferation rate) values encountered in these Pc patients confirm that AgNOr analysis may be regarded as an additional tool when the pathologist encounters difficulties in defining parathyroid lesions which are not clearly benign.

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Significance of AgNOR counts for distinguishing carcinoma from adenoma and hyperplasia in parathyroid gland

Cited by 13 publications

References 25 publications

Histomorphometry and Quantification of Nucleolar Organizer Regions in Bovine Thyroid Containing Methylthiouracil Residues

Histomorphometry and Quantification of Nucleolar Organizer Regions in Bovine Thyroid Containing Methylthiouracil Residues

Relationship of AgNOR counts and nuclear DNA content to survival in patients with parathyroid carcinoma

Parathyroid carcinoma as a challenging diagnosis: Report of three cases

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