Significance and Role of Hormone Detection Methods in the Development of Endocrinology as a Biological Discipline

Abstract

This review covers the development of technology and methods of hormone detection in the human biological environment: from radioimmunological technique to various immune-enzyme methods. Modern ultrasensitive methods of immuneanalysis, which function on the principle of luminescent or fluorescent signal detection, are described in the article. In the recent years physiochemical ultrasensitive methods of detection using mass-spectrometry in combination with high resolution liquid chromatography are being more acknowledged and in perspective will become the leading technique of biological detection. This article also lists main achievements reached using aforesaid methods of hormone detection both in fundamental and in clinical endocrinology.

Pathogenetic Mechanisms of Relationship of Metabolic and Morphofunctional Disorders of Thyroid and Adrenal Glands in Diabetes Mellitus and Obesity

“…Material for morphological examination and autopsy material was stained with hematoxylin and eosin, iron hematoxylin and Masson's method, the sections were examined in an Opton light microscope and photographed [10], [11].…”