Signicance of Philadelphia Chromosome in Chronic Myeloid Leukemia Patients of Anmol Hospital, Lahore, Pakistan

Abstract

Chronic myelogenous Leukemia is (Clonal stem cell disorder) cancer which starts in bone marrow a soft tissue inside bones that aids to form blood cells. It is rst form of cancer that was rstly recognize to associate strongly with the chromosomal abnormality and the chromosome [t (9; 22) translocation] called Philadelphia chromosome. Abstract: Objective: Philadelphia chromosome is a characteristic chromosomal marker that is associated with chronic myelogenous leukemia. Methods: More than one hundred patients of either sex were selected for the experiment. RNA was isolated from whole blood of patients so can use exclusively in RT-PCR. Results: Philadelphia chromosome in blood samples of patients with suspected diagnosis of CML was detected in 63% of patients. During our experimental studies on CML patients we do not encounter any complex translocation involving chromosome 8, 9 and 22. Conclusions: Philadelphia chromosome is a precise cytogenetic marker the detection of which is signicant for differential diagnosis and clinical organization of patients with clinical diagnosis of CML. It is of signicant that Ph chromosome occurs in pre-leukemic stage and has great diagnostic signicance.