Signatures of north-eastern expansion and multiple refugia: genomic phylogeography of the Pine Barrens tree frog,Hyla andersonii(Anura: Hylidae)

Abstract

Range fragmentation poses challenges for species persistence over time and can be caused by both historical and contemporary processes. We combined genomic data, phylogeographical model testing and palaeoclimatic niche modelling to infer the evolutionary history of the Pine Barrens tree frog (Hyla andersonii), a seepage bog specialist, in eastern North America to gain a better understanding of the historical context of its fragmented distribution. We sampled H. andersonii populations across the three disjunct regions of the species range: Alabama/Florida (AF), the Carolinas (CL) and New Jersey (NJ). Phylogenetic relationships within H. andersonii were consistent between the nuclear species tree and mitochondrial analyses, indicating divergence between AF and CL/NJ (Atlantic clade) ~0.9 Mya and divergence of the NJ clade ~0.15 Mya. Several predictions of north-eastern expansion along the Atlantic coast were supported by phylogeographical analyses. Model testing using genome-wide single nucleotide polymorphism data and species distribution models both provided evidence for multiple disjunct refugia. This comprehensive phylogeographical study of H. andersonii demonstrates a long history of range fragmentation within an endemic coastal plain species and highlights the influence of historical climate change on the current distribution of species and their genetic diversity.

Characterization of Two Mitogenomes of Hyla sanchiangensis (Anura: Hylidae), with Phylogenetic Relationships and Selection Pressure Analyses of Hylidae

“…The gene arrangements were the same as the mitogenome patterns of other Hyla and Dryophytes species [26,27,31,[59][60][61][62][63][64][65]. Comparing the two mitogenome sequences produced in our study to a previous mitogenome of H. sanchiangensis from Lishui (MT561180) [31], subtle differences were found.…”

“…The secondary structure of tRNAs in the two mitogenome sequences contained non-Waston-Crick base pairs in the structure of stems, which was similar to the mitogenome of the individual collected in Lishui [31]. There were many overlapping regions in the three mitogenomes of H. sanchiangensis, with the longest overlapping region (25 bp) being between ATP8 and ATP6, which was also found in all mitogenome sequences of Hyla species [31,[62][63][64][65], whereas only a 10 bp overlap region was found in the Dryophytes sequences [26,27,[59][60][61]. This further illustrated the differences between the genera Hyla and Dryophytes and also supported the result expounded by Zhang et al [26].…”

Differential Mitochondrial Genome Expression of Four Hylid Frog Species under Low-Temperature Stress and Its Relationship with Amphibian Temperature Adaptation

“…mdpi.com/article/10.3390/ijms25115967/s1. References [111][112][113][114][115][116] are cited in the Supplementary Materials.…”

“…The following supporting information can be downloaded at: . References [ 111 , 112 , 113 , 114 , 115 , 116 ] are cited in the Supplementary Materials.…”

Projected northward shifts in eastern red‐backed salamanders due to changing climate

“…Conserving species requires protecting the habitat within their distributions (Lomolino, 2004 ; Real et al, 2009 ). Species distribution models (SDMs) and ecological niche models are useful tools for understanding how a species' range may overlap with those of other species (Caravaggi et al, 2017 ; Real et al, 2009 ), how it has changed in the past (Gutiérrez‐Rodríguez et al, 2017a , 2017b ; Warwick et al, 2021 ), and how it may change in the future under different climate projections (Báez et al, 2020 ; Pearson & Dawson, 2003 ; Real et al, 2010 ; Zhang et al, 2020 ), which may potentially lead to future species range expansions and contractions. SDMs have been used extensively to assess climate impacts on amphibian ranges (Barbosa & Real, 2012 ; Gutiérrez‐Rodríguez et al, 2017a , 2017b ; Reino et al, 2017 ; Sánchez‐Montes et al, 2019 ), and it has been found that ectotherm distributions are more strongly and directly impacted by climactic changes, likely as a result of physiological constraints, compared with endotherms, which are more affected by indirect climate effects (Aragón et al, 2010 ).…”