Signalling of abscisic acid to regulate plant growth

Himmelbach, Axel; Iten, Monika; Grill, Erwin

doi:10.1098/rstb.1998.0299

Cited by 89 publications

(81 citation statements)

References 84 publications

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“…Analysis of transgenic plants with altered sumoylation levels suggested that SUMO modulates the signaling of the hormone abscisic acid (ABA), which mediates plant responses to environmental stresses such as cold, drought, and high salinity (Leung and Giraudat, 1998). During vegetative growth, endogenous ABA levels increased in response to these adverse environmental stimuli, and histochemical studies of root tissues supported the notion of an ABA-mediated inhibition of cell elongation and an arrest in mitotic cell activity (Himmelbach et al, 1998). Plants with increased sumoylation levels showed an attenuation of ABA-mediated growth inhibition, and the opposite effect was observed when AtSCE1a levels were reduced.…”

Section: Introductionmentioning

confidence: 80%

Small Ubiquitin-Like Modifier Modulates Abscisic Acid Signaling in Arabidopsis

2003

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Post-translational modification of proteins by small polypeptides, such as ubiquitin, has emerged as a common and important mechanism for regulating protein function. Small ubiquitin-like modifier (SUMO) is a small protein that is structurally related to but functionally different from ubiquitin. We report the identification and functional analysis of AtSUMO1, AtSUMO2, and AtSCE1a as components of the SUMO conjugation (sumoylation) pathway in Arabidopsis. In yeast-two hybrid assays, AtSUMO1/2 interacts specifically with a SUMO-conjugating enzyme but not with a ubiquitin-conjugating enzyme. AtSCE1a, the Arabidopsis SUMO-conjugating enzyme ortholog, conjugates SUMO to RanGAP in vitro. AtSUMO1/2 and AtSCE1a colocalize at the nucleus, and AtSUMO1/2 are conjugated to endogenous SUMO targets in vivo. Analysis of transgenic plants showed that overexpression of AtSUMO1/2 does not have any obvious effect in general plant development, but increased sumoylation levels attenuate abscisic acid (ABA)-mediated growth inhibition and amplify the induction of ABA-and stress-responsive genes such as RD29A . Reduction of AtSCE1a expression levels accentuates ABA-mediated growth inhibition. Our results suggest a role for SUMO in the modulation of the ABA signal transduction pathway.

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Section: Introductionmentioning

confidence: 80%

Small Ubiquitin-Like Modifier Modulates Abscisic Acid Signaling in Arabidopsis

2003

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“…ABA has an inhibitory effect on root growth and, consequently, ABA-insensitive mutants and FsPP2C1-overexpressing plants are resistant to this ABA-mediated process (Himmelbach et al, 1998;González-García et al, 2003). Twelve-day-old 35S: PYL8/RCAR3 seedlings grown in the presence of 10 and 30 mM ABA showed an enhanced inhibition of root growth compared with wild-type plants and to a greater extent with that of abi mutants (Fig.…”

Section: Effect Of Pyl8/rcar3 Overexpression In Vegetative Tissuesmentioning

confidence: 99%

The Nuclear Interactor PYL8/RCAR3 ofFagus sylvaticaFsPP2C1 Is a Positive Regulator of Abscisic Acid Signaling in Seeds and Stress

et al. 2009

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The functional protein phosphatase type 2C from beechnut (Fagus sylvatica; FsPP2C1) was a negative regulator of abscisic acid (ABA) signaling in seeds. In this report, to get deeper insight on FsPP2C1 function, we aim to identify PP2C-interacting partners. Two closely related members (PYL8/RCAR3 and PYL7/RCAR2) of the Arabidopsis (Arabidopsis thaliana) BetV I family were shown to bind FsPP2C1 in a yeast two-hybrid screening and in an ABA-independent manner. By transient expression of FsPP2C1 and PYL8/RCAR3 in epidermal onion (Allium cepa) cells and agroinfiltration in tobacco (Nicotiana benthamiana) as green fluorescent protein fusion proteins, we obtained evidence supporting the subcellular localization of both proteins mainly in the nucleus and in both the cytosol and the nucleus, respectively. The in planta interaction of both proteins in tobacco cells by bimolecular fluorescence complementation assays resulted in a specific nuclear colocalization of this interaction. Constitutive overexpression of PYL8/RCAR3 confers ABA hypersensitivity in Arabidopsis seeds and, consequently, an enhanced degree of seed dormancy. Additionally, transgenic 35S:PYL8/RCAR3 plants are unable to germinate under low concentrations of mannitol, NaCl, or paclobutrazol, which are not inhibiting conditions to the wild type. In vegetative tissues, Arabidopsis PYL8/RCAR3 transgenic plants show ABA-resistant drought response and a strong inhibition of early root growth. These phenotypes are strengthened at the molecular level with the enhanced induction of several ABA response genes. Both seed and vegetative phenotypes of Arabidopsis 35S:PYL8/RCAR3 plants are opposite those of 35S: FsPP2C1 plants. Finally, double transgenic plants confirm the role of PYL8/RCAR3 by antagonizing FsPP2C1 function and demonstrating that PYL8/RCAR3 positively regulates ABA signaling during germination and abiotic stress responses.

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“…Application of exogenous ABA is well established to affect root growth as an antagonist of auxin, impairing cell elongation and causing an arrest in mitotic cell cycle activity (Himmelbach et al, 1998). The ABAinsensitive dominant abi1-1 mutant and sustained ex- Figure 2A for reference purposes.…”

Section: Atpp2ca a Negative Regulator Of Physiological Aba Responsesmentioning

confidence: 99%

“…To date, genetic screens for ABA-hypersensitive mutants have indicated that processes including farnesylation (era1; Cutler et al, 1996;Pei et al, 1998), inositol 1,4,5-triphosphate (IP 3 ) dephosphorylation (fry1; Xiong et al, 2001b), and RNA metabolism (hyl1; Lu and Fedoroff, 2000;abh1, Hugouvieux et al, 2001;sad1, Xiong et al, 2001a) are required to attenuate the ABA signal. However, surprisingly few non-transcription factor-encoding genes have been identified as recessive ABA-insensitive disruption mutants, namely, the G-protein a-subunit GPA1 , the RCN1 protein phosphatase type 2A subunit , the OST1/ SnRK2E protein kinase (Mustilli et al, 2002;Yoshida et al, 2002), the AtRBOHD/F NADPH oxidases (Kwak et al, 2003), ABI8 (Brocard-Gifford et al, 2004), RPK1 (Osakabe et al, 2005), and GCA2 (Himmelbach et al, 1998).…”

mentioning

confidence: 99%

The Protein Phosphatase AtPP2CA Negatively Regulates Abscisic Acid Signal Transduction in Arabidopsis, and Effects of abh1 on AtPP2CA mRNA

et al. 2005

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To identify new loci in abscisic acid (ABA) signaling, we screened a library of 35STcDNA Arabidopsis (Arabidopsis thaliana)-expressing lines for ABA-insensitive mutants in seed germination assays. One of the identified mutants germinated on 2.5 mM ABA, a concentration that completely inhibits wild-type seed germination. Backcrosses and F 2 analyses indicated that the mutant exhibits a dominant phenotype and that the ABA insensitivity was linked to a single T-DNA insertion containing a 35STcDNA fusion. The inserted cDNA corresponds to a full-length cDNA of the AtPP2CA gene, encoding a protein phosphatase type 2C (PP2C). Northern-blot analyses demonstrated that the AtPP2CA transcript is indeed overexpressed in the mutant (named PP2CAox). Two independent homozygous T-DNA insertion lines, pp2ca-1 and pp2ca-2, were recovered from the Arabidopsis Biological Resource Center and shown to lack full-length AtPP2CA expression. A detailed characterization of PP2CAox and the T-DNA disruption mutants demonstrated that, whereas ectopic expression of a 35STAtPP2CA fusion caused ABA insensitivity in seed germination and ABA-induced stomatal closure responses, disruption mutants displayed the opposite phenotype, namely, strong ABA hypersensitivity. Thus our data demonstrate that the PP2CA protein phosphatase is a strong negative regulator of ABA signal transduction. Furthermore, it has been previously shown that the AtPP2CA transcript is down-regulated in the ABAhypersensitive nuclear mRNA cap-binding protein mutant abh1. We show here that down-regulation of AtPP2CA in abh1 is not due to impaired RNA splicing of AtPP2CA pre-mRNA. Moreover, expression of a 35STAtPP2CA cDNA fusion in abh1 partially suppresses abh1 hypersensitivity, and the data further suggest that additional mechanisms contribute to ABA hypersensitivity of abh1.

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Signalling of abscisic acid to regulate plant growth

Cited by 89 publications

References 84 publications

Small Ubiquitin-Like Modifier Modulates Abscisic Acid Signaling in Arabidopsis

Small Ubiquitin-Like Modifier Modulates Abscisic Acid Signaling in Arabidopsis

The Nuclear Interactor PYL8/RCAR3 ofFagus sylvaticaFsPP2C1 Is a Positive Regulator of Abscisic Acid Signaling in Seeds and Stress

The Protein Phosphatase AtPP2CA Negatively Regulates Abscisic Acid Signal Transduction in Arabidopsis, and Effects of abh1 on AtPP2CA mRNA

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