Signalling components of the house mouse mate recognition system

Bímová, Barbora Vošlajerová; Albrecht, Tomáš; Macholán, Miloš; Piálek, Jaroslav

doi:10.1016/j.beproc.2008.08.004

Cited by 27 publications

(33 citation statements)

References 63 publications

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“…However, when experimentally tested, wild mice of the two subspecies did not show any difference in time spent sniffing urine to sniffing the urine with added ABPs18. In our data, some SCGBs/ABPs were male-biased but no member of this family was sex unique in the mouse saliva.…”

Section: Discussioncontrasting

confidence: 50%

“…Thus, lipocalins and their specific ligands together form a signal15. Differential ligand binding may have a potential influence on sub-species recognition between M. m. musculus and M. m. domesticus 161718. These two sub-species have been previously shown to vary in the abundance of male VOCs19 and in MUP expression between the two subspecies and individuals of the opposite sex9.…”

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confidence: 99%

“…These two sub-species have been previously shown to vary in the abundance of male VOCs19 and in MUP expression between the two subspecies and individuals of the opposite sex9. Moreover, scent signals have been shown to be an integral part of subspecies recognition and could play important roles in preventing interspecific mating between the two house mouse subspecies18.…”

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confidence: 99%

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On the saliva proteome of the Eastern European house mouse (Mus musculus musculus) focusing on sexual signalling and immunity

Stopka

Kuntová

Klempt

et al. 2016

Sci Rep

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Chemical communication is mediated by sex-biased signals abundantly present in the urine, saliva and tears. Because most studies concentrated on the urinary signals, we aimed to determine the saliva proteome in wild Mus musculus musculus, to extend the knowledge on potential roles of saliva in chemical communication. We performed the gel-free quantitative LC-MS/MS analyses of saliva and identified 633 proteins with 134 (21%) of them being sexually dimorphic. They include proteins that protect and transport volatile organic compounds in their beta barrel including LCN lipocalins, major urinary proteins (MUPs), and odorant binding proteins (OBPs). To our surprise, the saliva proteome contains one MUP that is female biased (MUP8) and the two protein pheromones MUP20 (or ‘Darcin’) and ESP1 in individuals of both sex. Thus, contrary to previous assumptions, our findings reveal that these proteins cannot function as male-unique signals. Our study also demonstrates that many olfactory proteins (e.g. LCNs, and OBPs) are not expressed by submandibular glands but are produced elsewhere–in nasal and lacrimal tissues, and potentially also in other oro-facial glands. We have also detected abundant proteins that are involved in wound healing, immune and non-immune responses to pathogens, thus corroborating that saliva has important protective roles.

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Section: Discussioncontrasting

confidence: 50%

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confidence: 99%

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confidence: 99%

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On the saliva proteome of the Eastern European house mouse (Mus musculus musculus) focusing on sexual signalling and immunity

Stopka

Kuntová

Klempt

et al. 2016

Sci Rep

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“…This study addresses the signal component of potential hybrid mates, by assessing how such signals are perceived, mainly by the choosiest subspecies (musculus) [16,34,36].…”

Section: Introductionmentioning

confidence: 99%

Sexual selection against natural hybrids may contribute to reinforcement in a house mouse hybrid zone

et al. 2014

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Sexual selection may hinder gene flow across contact zones when hybrid recognition signals are discriminated against. We tested this hypothesis in a unimodal hybrid zone between Mus musculus musculus and Mus musculus domesticus where a pattern of reinforcement was described and lower hybrid fitness documented. We presented mice from the border of the hybrid zone with a choice between opposite sex urine from the same subspecies versus hybrids sampled in different locations across the zone. While no preference was evidenced in domesticus mice, musculus males discriminated in favour of musculus signals and against hybrid signals. Remarkably, the pattern of hybrid unattractiveness did not vary across the hybrid zone. Moreover, allopatric populations tested in the same conditions did not discriminate against hybrid signals, indicating character displacement for signal perception or preference. Finally, habituation-discrimination tests assessing similarities between signals pointed out that hybrid signals differed from the parental ones. Overall, our results suggest that perception of hybrids as unattractive has evolved in border populations of musculus after the secondary contact with domesticus. We discuss the mechanisms involved in hybrid unattractiveness, and the potential impact of asymmetric sexual selection on the hybrid zone dynamics and gene flow between the two subspecies.

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“…The analysis of the total times spent sniffing each signal, analyzed here as coefficient of preference of the signal (R Sn ) ( Table 3), shows that both males and females preferred saliva samples of the +/+ genotype over those of the 2/2 genotype (R Sn , females: t-test: t = 2.178, P = 0.037; males: t-test: t = 2.309, P = 0.028). Stronger preferences displayed in close contact with a signal cue may indicate the role of ABP as a nonvolatile signal similar to MUPs (Bímová et al 2009). However, based on these data, we cannot speculate about the signaling mechanism of ABPs.…”

Section: Male and Female Preference For Knockout Genotype Salivamentioning

confidence: 99%

Studies of an Androgen-Binding Protein Knockout Corroborate a Role for Salivary ABP in Mouse Communication

et al. 2017

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The house mouse Androgen-binding protein (Abp) gene family is comprised of 64 paralogs, 30 Abpa and 34 Abpbg, encoding the alpha (ABPA) and beta-gamma (ABPBG) protein subunits that are disulfide-bridged to form dimers in secretions. Only 14 Abp genes are expressed in distinct patterns in the lacrimal (11) and submandibular glands (3). We created a knockout mouse line lacking two of the three genes expressed in submandibular glands, Abpa27 and Abpbg27, by replacing them with the neomycin resistance gene. The knockout genotype (2/2) showed no Abpa27 or Abpbg27 transcripts in submandibular gland complementary DNA (cDNA) libraries and there was a concomitant lack of protein expression of ABPA27 and ABPBG27 in the 2/2 genotype saliva, shown by elimination of these two proteins from the saliva proteome and the loss of cross-reactive material in the acinar cells of the submandibular glands. We also observed a decrease in BG26 protein in the 2/2 animals, suggesting monomer instability. Overall, we observed no major phenotypic changes in the 2/2 genotype, compared with their +/+ and +/2 siblings raised in a laboratory setting, including normal growth curves, tissue histology, fecundity, and longevity. The only difference is that male and female C57BL/6 mice preferred saliva of the opposite sex containing ABP statistically significantly more than saliva of the opposite sex without ABP in a Y-maze test. These results show for the first time that mice can sense the presence of ABP between saliva targets with and without ABPs, and that they spend more time investigating the target containing ABP.

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Signalling components of the house mouse mate recognition system

Cited by 27 publications

References 63 publications

On the saliva proteome of the Eastern European house mouse (Mus musculus musculus) focusing on sexual signalling and immunity

On the saliva proteome of the Eastern European house mouse (Mus musculus musculus) focusing on sexual signalling and immunity

Sexual selection against natural hybrids may contribute to reinforcement in a house mouse hybrid zone

Studies of an Androgen-Binding Protein Knockout Corroborate a Role for Salivary ABP in Mouse Communication

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