Signaling via cAMP in fungi: interconnections with mitogen-activated protein kinase pathways

Kronstad, James W.; Maria, Adriana De; Funnell, Deanna L.; Laidlaw, R. David; Lee, Nancy; Sá, Mário Moniz de; Ramesh, Marilee A.

doi:10.1007/s002030050659

Cited by 171 publications

(107 citation statements)

References 69 publications

(101 reference statements)

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“…It was reported that ultrasound mediates the metabolism of mouse cells through mitogen-activated protein kinases (MAPKs) pathway [40]. MAPKs have also been characterized in different plant and fungi and appear to function coordinately to regulate the transcription factors and the expression of specific sets of genes in response to environmental stress [41,42]. Putative MAPKs have been found in the gene sequence of T. versicolor [43].…”

Section: Effect Of the Ultrasonic Treatments On The Expression Of Lccmentioning

confidence: 99%

Ultrasound-intensified laccase production from Trametes versicolor

Wang

Chen

et al. 2013

Ultrasonics Sonochemistry

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a b s t r a c tAn efficient intermittent ultrasonic treatment strategy was developed to improve laccase production from Trametes versicolor mycelia cultures. The optimized strategy consisted of exposing 2-day-old mycelia cultures to 5-min ultrasonic treatments for two times with a 12-h interval at the fixed ultrasonic power and frequency (120 W, 40 kHz). After 5 days of culture, this strategy produced the highest extracellular laccase activity of 588.9 U/L among all treatments tested which was 1.8-fold greater than the control without ultrasound treatment. The ultrasonic treatment resulted in a higher pellet porosity that facilitated the mass transfer of nutrients and metabolites from the pellets to the surrounding liquid. Furthermore, the ultrasonic treatment induced the expression of the laccase gene (lcc), which correlated with a sharp increase in both extracellular and intracellular laccase activity. This is the first study to find positive effects of ultrasound on gene expression in fungal cells. These results provide a basis for understanding the stimulation of metabolite production and process intensification by ultrasonic treatment in filamentous fungal culture.

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Section: Effect Of the Ultrasonic Treatments On The Expression Of Lccmentioning

confidence: 99%

Ultrasound-intensified laccase production from Trametes versicolor

Wang

Chen

et al. 2013

Ultrasonics Sonochemistry

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“…Recently, results derived from genetic approaches have indicated that the signalling systems controlling filamentous growth are multiple and conserved between distantly related fungi. In particular, cAMP signalling has been demonstrated to mediate fungal development, although target effectors have not yet been suggested (Kronstad, 1997 ;Alspaugh et al, 1998 ;Hamer & Talbot, 1998 ;Kronstad et al, 1998 ;Madhani & Fink, 1998 ;Yang & Dickman, 1999 (Bartnicki-Garcı! a & Nickerson, 1962).…”

Section: Introductionmentioning

confidence: 99%

Threshold level of protein kinase A activity and polarized growth in Mucor rouxii

2000

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A model system to study the involvement of cAMP-mediated regulation of a cellular process such as hyphal morphogenesis was investigated. Impairment of polarized growth was observed when Mucor rouxii sporangiospores were grown in the presence of N 6 -cAMP analogues and of SQ 65,442, a cAMP phosphodiesterase inhibitor. Together with an effect on isodiametric growth, there was increased pigmentation, increased cell fragility and loss of cell adhesiveness. The total effect on morphology was attained even after adding the compounds shortly before germ-tube emergence ; when added after this time growth continued in a non-polarized form and rounding of the germ tip was observed. The morphological effect was observed under all the nutritional and environmental conditions studied (aerobic conditions and defined medium with maltose or glucose, Casamino acids medium with glucose, or rich medium ; anaerobic conditions with rich medium ; and following a shift from anaerobiosis to aerobiosis). The time of germ-tube emergence, and the size of the cell at this time, was dependent on the growth medium. Protein kinase A (PKA) specific activity was followed during the germination process under three growth conditions. It was found that the total activity of PKA correlated with differentiation and not with growth, and that the total specific activity at the time of germination was the same, independent of the culture medium. The time of germ-tube emergence correlated with the time of attainment of a threshold level of PKA total specific activity. The concentration of dibutyrylcAMP needed to promote isodiametric growth correlated with the total units of activity of PKA to be activated per cell. It was concluded that PKA is involved in the morphogenetic process of the fungus grown under all the nutritional and ambient conditions tested.

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“…In S. pombe, the MAP kinase Sty1 pathway participates in the maintenance of viability of starved cells (Kronstad et al, 1998). Thus, during transition to stationary phase in glucose-limiting conditions, Sty1 becomes activated and triggers a transcriptional stress programme, whereas such activation does not occur under glucose-rich conditions (Zuin et al, 2010;Zuin et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

New roles of the fission yeast eIF2α kinases Hri1 and Gcn2 in response to nutritional stress

Martín

Berlanga

Haro

2013

Journal of Cell Science

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SummaryIn fission yeast, three distinct eukaryotic initiation factor 2a (eIF2a) kinases (Hri1, Hri2 and Gcn2), regulate protein synthesis in response to various environmental stresses. Thus, Gcn2 is activated early after exposure to hydrogen peroxide (H 2 O 2 ) and methyl methanesulfonate (MMS), whereas Hri2 is the primary activated eIF2a kinase in response to heat shock. The function of Hri1 is still not completely understood. It is also known that the mitogen-activated protein kinase Sty1 negatively regulates Gcn2 and Hri2 activities under oxidative stress. In this study, we demonstrate that Hri1 is mainly activated, and its expression upregulated, during transition from exponential growth to the stationary phase in response to nutritional limitation. Accordingly, both Hri1 and Gcn2, but not Hri2, are activated upon nitrogen source deprivation. In contrast, Hri2 is stimulated early during glucose starvation. We also found that Gcn2 is implicated in nitrogen starvation-induced growth arrest in the cell cycle G1 phase as well as in the non-selective protein degradation process caused upon this particular cellular stress. Moreover, Gcn2, but not Hri1 or Hri2, is essential for survival of cells growing in minimal medium, upon oxidative stress or glucose limitation. We further show that eIF2a phosphorylation at serine 52 by the eIF2a kinases is necessary for efficient cell cycle arrest in the G1 phase, for the consequent protein degradation and for sexual differentiation, under nitrogen starvation. Therefore, the eIF2a kinase signalling pathway modulates G1 phase cell cycle arrest, cell survival and mating under nutritional stress in the fission yeast Schizosaccharomyces pombe.

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Signaling via cAMP in fungi: interconnections with mitogen-activated protein kinase pathways

Cited by 171 publications

References 69 publications

Ultrasound-intensified laccase production from Trametes versicolor

Ultrasound-intensified laccase production from Trametes versicolor

Threshold level of protein kinase A activity and polarized growth in Mucor rouxii

New roles of the fission yeast eIF2α kinases Hri1 and Gcn2 in response to nutritional stress

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