Signaling Reactions Induced in Human Fibroblasts During Adhesion to Cementum-Derived Attachment Protein

Saito, Masahiro; Narayanan, Sampath

doi:10.1359/jbmr.1999.14.1.65

Cited by 24 publications

(40 citation statements)

References 43 publications

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“…Cas is now considered to be a significant adaptor molecule in a variety of biological processes, including cell adhesion (38), migration (39), growth factor stimulation (40), and cytokine receptor engagement (41). Paxillin is a multidomain protein that primarily localizes to cell adhesions forming a linkage structure between the ECM and the actin cytoskeleton, and is also an important site for signal transduction (42), particularly in melanomas (43).…”

Section: Discussionmentioning

confidence: 99%

Ganglioside GD3 Enhances Adhesion Signals and Augments Malignant Properties of Melanoma Cells by Recruiting Integrins to Glycolipid-enriched Microdomains

Ohkawa

Miyazaki

Hamamura

et al. 2010

Journal of Biological Chemistry

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Ganglioside GD3 is widely expressed in human malignant melanoma cell lines and tumors. Previously, we reported that GD3؉ cells show stronger tyrosine phosphorylation of focal adhesion kinase (FAK), p130Cas , and paxillin when treated with fetal calf serum than GD3؊ cells. In this study, we analyzed the changes in the signals mediated by the interaction between integrins and extracellular matrices (ECM) to clarify how GD3 enhances cell signals in the vicinity of the cell membrane. An adhesion assay with a real time cell electronic sensing system revealed that GD3؉ cells had stronger adhesion to all extracellular matrices examined. In particular, GD3؉ cells attached more strongly to collagen type I and type IV than controls. Correspondingly, they showed stronger tyrosine phosphorylation of FAK and paxillin during adhesion to collagen type I. In the floating pattern of detergent extracts, a high level of integrin ␤1 was found in glycolipid-enriched microdomain (GEM)/rafts in GD3؉ cells before adhesion, whereas a smaller amount of integrin ␤1 was detected in the GEM/rafts of controls. Some phosphorylated forms of FAK as well as total FAK were found in GEM/rafts during cell adhesion only in GD3؉ cells. Another signal consisting of integrin-linked kinase/Akt was also activated during adhesion more strongly in GD3؉ cells than in controls. In double stained GD3؉ cells, GD3 and integrin ␤1 colocalized at the focal adhesion with a punctate pattern. All these results suggested that integrins assembled and formed a cluster in GEM/rafts, leading to the enhanced signaling and malignant properties under GD3 expression.

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Section: Discussionmentioning

confidence: 99%

Ganglioside GD3 Enhances Adhesion Signals and Augments Malignant Properties of Melanoma Cells by Recruiting Integrins to Glycolipid-enriched Microdomains

Ohkawa

Miyazaki

Hamamura

et al. 2010

Journal of Biological Chemistry

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“…Differential activation of Erk-1\2 has been observed in other cell systems. For example, in human fibroblasts adherent to cementum-derived attachment protein only Erk-2 was phosphorylated (Saito and Narayana, 1999). Analysis of changes in phosphorylation of Erk-1\2 during TPA-induced differentiation of human promyelocytic leukemia cells suggested that the two MAPkinases are regulated by distinct mechanisms (Meighan-Mantha et al, 1997).…”

Section: Discussionmentioning

confidence: 99%

Evidence for Differential Signaling in Human Conjunctival Epithelial Cells Adherent to Laminin Isoforms

Lin

Daneshvar

Kurpakus-Wheater

2000

Experimental Eye Research

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“…The differences in the onset and time course of collagen I expression between the periodontal bone and some primary osteoblast cultures is most likely due to the variability of phe- (Rodan, 1981;McCulloch et al, 1987McCulloch et al, , 1989McCulloch and Heersche, 1988 (Pavlin et al, 1998(Pavlin et al, , 2001a (TakanoYamamoto et al, 1994), BSP (Lekic et al, 1996), and osteonectin (Tung et al, 1985)-are also expressed in cementoblasts (MacNeil et al, 1995;D'Errico et al, 1997), while other markers, such as cementum-derived attachment protein and cementumderived growth factor, are specifically associated with cementoblasts (Arzate et al, 1992;Yonemura et al, 1992Yonemura et al, , 1993Pitaru et al, 1993;Liu et al, 1997;Metzger et al, 1998;Saito and Narayanan, 1999). However, there are several differences between bone and cementum, the most obvious being the lack of vascularity and innervation in cementum.…”

Section: (I) Introductionmentioning

confidence: 99%

Effect of Mechanical Loading On Periodontal Cells

Pavlin

Gluhak-Heinrich²

2001

Critical Reviews in Oral Biology & Medicine

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Mechanical loading is an important regulatory factor in alveolar bone homeostasis, and plays an essential role in maintaining the structure and mass of the alveolar processes throughout lifetime. A better understanding of the cellular and molecular responses of periodontal cells is a prerequisite for further improvements of therapeutic approaches in orthodontics, periodontal and alveolar bone repair and regeneration, implantology, and post-surgical wound healing. The purpose of this review is to provide an insight into some cell culture and animal models used for studying the effects of mechanical loading on periodontal cells, and into the recent developments and utilization of new in vivo animal models. There has been an increased awareness about the need for improvement and development of in vivo models to supplement the widely used cell culture models, and for biological validation of in vitro results, especially in the light of evidence that developmental models may not always reflect bone homeostasis in an adult organism. Due to the limitations of in vivo models, previous studies on mechanical regulation of alveolar bone osteoblasts and cementoblasts mostly focused on proliferative responses, rather than on the stimulation of cell differentiation. To address this problem, we have recently characterized and implemented a mouse osteoinductive tooth movement model for studying mechanically induced regulation of osteoblast-and cementoblast-associated genes. In this model, a defined and reproducible mechanical osteogenic loading is applied during a time course of up to two weeks. Regulation of gene expression in either wild-type or transgenic animals is assessed by a relative quantitative measurement of the level of target mRNAs directly within the subpopulations of periodontal cells. To date, results demonstrate a defined temporal pattern of cellspecific gene regulation in periodontal osteoblasts mechanically stimulated to differentiate and deposit bone matrix. The responses of osteoblast-associated genes to mechanical loading were 10-to 20-fold greater than the increase in the numbers of these cells, indicating that the induction of differentiation and an increase of cell function are the primary responses to osteogenic loading. The progression of the osteoblast phenotype in the intact mouse periodontium was several-fold faster compared with that in cultured cells, suggesting that the mechanical signal may be targeting osteoblast precursors in the state of readiness to respond to an environmental challenge, without the initial proliferative response. An early response of alkaline phosphatase and bone sialoprotein genes was detected after 24 hrs of treatment, followed by a concomitant stimulation of osteocalcin and collagen I between 24 and 48 hrs, and deposition of osteoid after 72 hrs. Although cementoblasts constitutively express biochemical markers similar to those of osteoblasts, distinct responses of osteocalcin, collagen I, and bone sialoprotein genes to mechanical loading were observed in the ...

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Signaling Reactions Induced in Human Fibroblasts During Adhesion to Cementum-Derived Attachment Protein

Cited by 24 publications

References 43 publications

Ganglioside GD3 Enhances Adhesion Signals and Augments Malignant Properties of Melanoma Cells by Recruiting Integrins to Glycolipid-enriched Microdomains

Ganglioside GD3 Enhances Adhesion Signals and Augments Malignant Properties of Melanoma Cells by Recruiting Integrins to Glycolipid-enriched Microdomains

Evidence for Differential Signaling in Human Conjunctival Epithelial Cells Adherent to Laminin Isoforms

Effect of Mechanical Loading On Periodontal Cells

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