“…Early experiments showed that LPHN1 can be copurified with Ga o (Lelianova et al, 1997), and stimulation with a-latrotoxin, a known LPHN1 ligand, evokes neurotransmitter release in the presence of extracellular calcium in a phospholipase C-dependent manner (Rahman et al, 1999). Other indirect functional evidence came from second messenger assays (Bohnekamp and Schöneberg, 2011;Gupte et al, 2012;Mogha et al, 2013;Liebscher et al, 2014b) and activated downstream components of G protein signaling cascades (Iguchi et al, 2008;Bohnekamp and Schöneberg, 2011;Yang et al, 2011;Ward et al, 2011;Giera et al, 2015).…”