Signaling pathways of the early differentiation of neural stem cells by neurotrophin-3

Lim, Myung-Shin; Nam, Sang-Hyun; Kim, Sun-Jung; Kang, Seog-Youn; Lee, Yong Soon; Kang, Kyung‐Sun

doi:10.1016/j.bbrc.2007.04.045

Cited by 47 publications

(35 citation statements)

References 26 publications

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“…Although it was demonstrated previously that all NTs can stimulate oligodendrocyte survival, NT-3 may be responsible for directing NSCs into an oligodendroglia fate (Kumar et al, 1998), in addition to the enhancement of neurogenesis (Lim et al, 2007). Hence, our results suggest that NT-mediated differentiation and proliferation in-vivo may be determined by the summation of signaling processes originating from the NT receptors shown to be expressed on NSCs including p75 NTR .…”

Section: Discussionmentioning

confidence: 58%

Brain-Derived Neurotrophic Factor (BDNF) has Proliferative Effects on Neural Stem Cells through the Truncated TRK-B Receptor, MAP Kinase, AKT, and STAT-3 Signaling Pathways

Islam

Loo

Heese

2009

CNR

147

101

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Neurospheres can be generated from the mouse fetal forebrain by exposing multipotent neural stem cells (NSCs) to epidermal growth factor (EGF). In the presence of EGF, NSCs can proliferate continuously while retaining the potential to differentiate into neurons, astrocytes and oligodendrocytes. We examined the expression pattern of the neurotrophin (NT) receptors tropomysin-related kinase (TRK)-A, TRK-B, TRK-C and p75 neurotrophin receptor (p75(NTR)) in NSCs and the corresponding lineage cells. Furthermore, we analyzed the action of the NT Brain-Derived Neurotrophic Factor (BDNF) on NSCs' behavior. The effects of BDNF on NSC proliferation and differentiation were examined together with the signaling pathways by which BDNF receptors transduce signaling effects. We found that all the known NT receptors, including the truncated isoforms of TRK-B (t-TRK-B) and TRK-C (t-TRK-C), were expressed by Nestin-positive cells within the neurosphere. Proliferation was enhanced in Nestin-positive and BrdU-positive cells in the presence of BDNF. In particular, we show that t-TRK-B was abundantly expressed in NSCs and the corresponding differentiated glia cells while full length TRK-B (fl-TRK-B) was expressed in fully differentiated post-mitotic neurons such as the neuronal cells of the newborn mouse cortex, suggesting that BDNF may exert its proliferative effects on NSCs through the t-TRK-B receptor. Finally, we analyzed the cell fates of NSCs differentiated with BDNF in the absence of EGF and we demonstrate that BDNF stimulated the formation of differentiated cell types in different proportions through the MAP kinase, AKT and STAT-3 signaling pathways. Thus, the in-vivo regulation of neurogenesis may be mediated by the summation of signals from the BDNF receptors, in particular the t-TRK-B receptor, regulating physiological fates as diverse as normal neural replacement, excessive neural loss, or tumor development.

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Section: Discussionmentioning

confidence: 58%

Brain-Derived Neurotrophic Factor (BDNF) has Proliferative Effects on Neural Stem Cells through the Truncated TRK-B Receptor, MAP Kinase, AKT, and STAT-3 Signaling Pathways

Islam

Loo

Heese

2009

CNR

147

101

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“…Recently, Chan et al [26] demonstrated that proliferation of NSPC in adult rat SC-NSPCs was regulated by ERK MAP kinase. Also, the p38 MAP kinase promotes differentiation of mouse NSPCs [54] and mediates the proliferative effects of FGF2 and the platelet-derived growth factor on oligodendrocyte progenitor cells [55]. Our findings suggest that the effects of SP on NSPC proliferation are mediated through the ERK and the p38 MAP kinase signaling pathway.…”

Section: Discussionmentioning

confidence: 83%

Substance P stimulates proliferation of spinal neural stem cells in spinal cord injury via the mitogen-activated protein kinase signaling pathway

Kim

Cho

et al. 2015

The Spine Journal

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“…BDNF was found to promote the differentiation of NSCs into GABAenergic neurons and stimulate the expression of TrkB receptors, which results in proliferation [23]. NT-3, along with the Trk C receptors in NSCs, might lead to the survival and neuronal differentiation of NSCs at an early stage of neuronal differentiation [24]. Thus, incorporation of both BDNF and NT-3 into collagen gel can significantly improve the proliferation and differentiation of NSCs.…”

Section: Discussionmentioning

confidence: 95%

Effect of controlled release of brain-derived neurotrophic factor and neurotrophin-3 from collagen gel on neural stem cells

Huang

Wu²,

Wang³

et al. 2016

NeuroReport

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This study aimed to examine the effect of controlled release of brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3) from collagen gel on rat neural stem cells (NSCs). With three groups of collagen gel, BDNF/collagen gel, and NT-3/collagen gel as controls, BDNF and NT-3 were tested in the BDNF-NT-3/collagen gel group at different time points. The enzyme-linked immunosorbent assay results showed that BDNF and NT-3 were steadily released from collagen gels for 10 days. The cell viability test and the bromodeoxyuridine incorporation assay showed that BDNF-NT-3/collagen gel supported the survival and proliferation of NSCs. The results also showed that the length of processes was markedly longer and differentiation percentage from NSCs into neurons was much higher in the BDNF-NT-3/collagen gel group than those in the collagen gel, BDNF/collagen gel, and NT-3/collagen gel groups. These findings suggest that BDNF-NT-3/collagen gel could significantly improve the ability of NSCs proliferation and differentiation.

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Signaling pathways of the early differentiation of neural stem cells by neurotrophin-3

Cited by 47 publications

References 26 publications

Brain-Derived Neurotrophic Factor (BDNF) has Proliferative Effects on Neural Stem Cells through the Truncated TRK-B Receptor, MAP Kinase, AKT, and STAT-3 Signaling Pathways

Brain-Derived Neurotrophic Factor (BDNF) has Proliferative Effects on Neural Stem Cells through the Truncated TRK-B Receptor, MAP Kinase, AKT, and STAT-3 Signaling Pathways

Substance P stimulates proliferation of spinal neural stem cells in spinal cord injury via the mitogen-activated protein kinase signaling pathway

Effect of controlled release of brain-derived neurotrophic factor and neurotrophin-3 from collagen gel on neural stem cells

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