Signaling pathways mediating the suppression of Arabidopsis thaliana Ku gene expression by abscisic acid

Liu, Pei‐Feng; Chang, Wen Chi; Wang, Yung Kai; Chang, Hwan‐You; Pan, Rong

doi:10.1016/j.bbagrm.2007.12.005

Cited by 19 publications

(10 citation statements)

References 54 publications

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“…We found that ABA treatment increased the HR frequency in both abo4-1 and wild-type plants. Interestingly, ABA downregulates the DNA end binding protein KU70, a key protein in NHEJ, which is consistent with a recent study on KU70 gene expression (Liu et al, 2008). It is possible that ABA-induced DNA breaks may partly result from the lower expression of KU70 because reduction of KU70 might lead to lower efficiently rejoining repair of DNA breaks in NHEJ.…”

Section: Discussionsupporting

confidence: 77%

Epigenetic Regulation, Somatic Homologous Recombination, and Abscisic Acid Signaling Are Influenced by DNA Polymerase ϵ Mutation inArabidopsis

et al. 2009

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Based on abscisic acid (ABA) inhibition of seed germination and seedling growth assays, we isolated an ABA overly sensitive mutant (abo4-1) caused by a mutation in the Arabidopsis thaliana POL2a/TILTED1(TIL1) gene encoding a catalytic subunit of DNA polymerase «. The dominant, ABA-insensitive abi1-1 or abi2-1 mutations suppressed the ABA hypersensitivity of the abo4-1 mutant. The abo4/til1 mutation reactivated the expression of the silenced Athila retrotransposon transcriptional silent information (TSI) and the silenced 35S-NPTII in the ros1 mutant and increased the frequency of somatic homologous recombination (HR) ;60-fold. ABA upregulated the expression of TSI and increased HR in both the wild type and abo4-1. MEIOTIC RECOMBINATION11 and GAMMA RESPONSE1, both of which are required for HR and double-strand DNA break repair, are expressed at higher levels in abo4-1 and are enhanced by ABA, while KU70 was suppressed by ABA. abo4-1 mutant plants are sensitive to UV-B and methyl methanesulfonate and show constitutive expression of the G2/Mspecific cyclin CycB1;1 in meristems. The abo4-1 plants were early flowering with lower expression of FLOWER LOCUS C and higher expression of FLOWER LOCUS T and changed histone modifications in the two loci. Our results suggest that ABO4/POL2a/TIL1 is involved in maintaining epigenetic states, HR, and ABA signaling in Arabidopsis.

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Section: Discussionsupporting

confidence: 77%

Epigenetic Regulation, Somatic Homologous Recombination, and Abscisic Acid Signaling Are Influenced by DNA Polymerase ϵ Mutation inArabidopsis

et al. 2009

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“…To determine if MYB30 functions in previously identified ABA signaling pathways, we crossed myb30-2 to abi1-3, abi2-2, abi3, abi4, and abi5-8 (all in Col-0) (37)(38)(39)(40)(41). Double-mutants of abi1-3 myb30-2 and abi2-2 myb30-2 exhibited enhanced ABA sensitivity relative to their single mutants, suggesting that MYB30 functions in parallel pathways with ABI1 or ABI2 during ABA signaling (Fig.…”

Section: Myb30 and Abi5 Regulate The Expression Of Largely Different mentioning

confidence: 99%

Sumoylation of transcription factor MYB30 by the small ubiquitin-like modifier E3 ligase SIZ1 mediates abscisic acid response in Arabidopsis thaliana

Zheng

Schumaker

Guo

2012

Proc. Natl. Acad. Sci. U.S.A.

178

176

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The phytohormone abscisic acid (ABA) plays an essential role in plant development and during the response of the plant to abiotic stress. In this study, we report that the R2R3-type transcription factor MYB30 is involved in the regulation of ABA signaling. Arabidopsis mutants lacking MYB30 are hypersensitive to ABA during germination and seedling growth. A K283R substitution in MYB30 blocks its SUMO E3 ligase SIZ1-mediated sumoylation in Arabidopsis protoplasts, indicating that MYB30 is sumoylated by SIZ1 and that K283 is the principal site for small ubiquitin-like modifier conjugation. Expression of MYB30 K283R in myb30 partially rescues the mutant ABA-hypersensitive phenotype, but expression of wild-type MYB30 complements the mutant phenotype. Overexpression of MYB30 in wild-type results in an ABA-insensitive phenotype, whereas overexpression of MYB30 in the siz1 mutant does not alter siz1 hypersensitivity to ABA. The siz1-2 myb30-2 double-mutant exhibits greater ABA sensitivity than either single mutant, but a mutation in the SIZ1-sumoylated ABI5 transcription factor suppresses the ABA hypersensitivity of myb30-2 to wild-type levels. Our results suggest that coordination of ABI5 and MYB30 sumoylation by SIZ1 may balance gene expression, which is required for regulation of ABA signaling during seed germination.A bscisic acid (ABA) plays important roles throughout the life cycle of the plant, including during seed development and dormancy, seed germination, early seedling development, flowering, and in response to abiotic and biotic stress. Genetic studies have revealed that a number of loci are essential for ABA biosynthesis (1-5), catabolism (6, 7), and signal transduction (8, 9) in plants.A number of studies have focused on understanding ABA signaling from perception to response. Recently, three groups of ABA receptors (ABAR/GUN5, GRGC/GTG, and PYR/PYL/ RCAR) have been identified in Arabidopsis and shown to bind ABA with high affinity (10-14). The various functions and cellular locations of these ABA receptors point to the complexity of ABA signaling. For example, PYR/PYL/RCAR-modulated ABA signaling has been studied extensively and found to involve a double-negative regulatory system that includes the receptor, protein phosphatases (PP2C), and protein kinases (SnRK2) (15, 16).A group of basic leucine zipper transcription factors, including ABI5, serve as the downstream targets of SnRK2 and phosphorylation is correlated with ABI5 transcriptional activity and protein stability (17-19). ABI5 undergoes 26S proteasome-mediated degradation and ABA stabilizes the ABI5 protein (17). Two ABI5 interacting proteins, AFP (ABI5-interacting protein) and KEG (KEEP ON GOING, a RING-finger ubiquitin E3 ligase) are required for ABI5 degradation (20,21). Arabidopsis lacking either gene is hypersensitive to ABA and an abi5 mutation suppresses the ABA sensitivity of both the afp-1 and keg mutants (20,21). Recently it has been shown that ABI5 is sumoylated by the small ubiquitin-like modifier (SUMO) E3 ligase SIZ1 at amino acid ...

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“…The DNA binding activity of proteins were determined by EMSA as described previously (Liu et al 2008a). The nonsequence-specific oligonucleotide probes were used and divided into several types: the sticky end of 20mer dsDNA, containing three-base overhangs at both end (5 0 -CAT GACTTGCGGCAATACAT-3 0 and 5 0 -TGCATGTATT GCCGCAAGTC-3 0 ); the blunt end of 20mer dsDNA (5 0 -CGTCAAATCTGCCGAAATAC-3 0 and 5 0 -GTATTTC GGCAGATTTGACG-3 0 ); the 20mer ssDNA (5 0 -CATG ACTTGCGGCAATACAT-3 0 or 5 0 -CGTCAAATCTGCCG AAATAC-3 0 , alternatively); the sticky end of 24mer dsDNA, containing three-base overhangs at both end (5 0 -CATGGAAGACTTGCGGCAATACAT-3 0 and 5 0 -TG CATGTATTGCCGCAAGTCTTCC-3 0 ).…”

Section: Electrophoretic Mobility Shift Assay (Emsa)mentioning

confidence: 99%

“…The AtKu gene is negatively regulated by the plant hormone abscisic acid (ABA) through extracellular Ca 2? , phospholipase D alpha (PLDa), p38-type mitogen-activated protein kinase (MAPK), MAPK6 and the ABA transcription factors ABI3 and ABI5 (Liu et al 2008a). Furthermore, in four-to five-week-old plants, the AtKu gene is down-regulated by heat stress in several specific tissues such as stems, rosette leaves and cauline leaves, whereas no difference in expression is seen in roots and primary inflorescences exposed to the same conditions (Liu et al 2008b).…”

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confidence: 97%

Ovate family protein 1 as a plant Ku70 interacting protein involving in DNA double-strand break repair

et al. 2010

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The Ku heterodimer, a DNA repair protein complex consisting of 70- and 80-kDa subunits, is involved in the non-homologous end-joining (NHEJ) pathway. Plants are thought to use the NHEJ pathway primarily for the repair of DNA double-strand breaks (DSBs). The Ku70/80 protein has been identified in many plants and been shown to possess several similar functions to its counter protein complex in mammals. In the present study, ovate family protein 1 (AtOFP1) was demonstrated to be a plant Ku-interacting protein by yeast two-hybrid screening and the GST pull-down assay. Truncation analysis revealed that the C-terminal domain of AtKu70 contains interacting sites for AtOFP1. The electrophoretic mobility shift assay (EMSA) indicated that AtOFP1 is also a DNA binding protein with its binding domain at the N-terminus. In 3-week-old seedlings, expression of the AtOFP1 gene increased after exposure to DNA-damaging agents (such as methyl methanesulfonate (MMS) and menadione) in a time dependent manner. Seedlings lacking the AtOFP1 protein were more sensitive to MMS and menadione as compared with wild-type. Furthermore, similar to AtKu70(-/-) and AtKu80(-/-), the AtOFP1(-/-) mutant showed relatively lower NHEJ activity in vivo. Taken together, these results suggest that AtOFP1 may play a role in DNA repair through the NHEJ pathway accompanying with the AtKu protein.

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Signaling pathways mediating the suppression of Arabidopsis thaliana Ku gene expression by abscisic acid

Cited by 19 publications

References 54 publications

Epigenetic Regulation, Somatic Homologous Recombination, and Abscisic Acid Signaling Are Influenced by DNA Polymerase ϵ Mutation inArabidopsis

Epigenetic Regulation, Somatic Homologous Recombination, and Abscisic Acid Signaling Are Influenced by DNA Polymerase ϵ Mutation inArabidopsis

Sumoylation of transcription factor MYB30 by the small ubiquitin-like modifier E3 ligase SIZ1 mediates abscisic acid response in Arabidopsis thaliana

Ovate family protein 1 as a plant Ku70 interacting protein involving in DNA double-strand break repair

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