Signaling pathway of globo-series glycosphingolipids and β1,3-galactosyltransferase V (β3GalT5) in breast cancer

Chuang, Po‐Kai; Hsiao, Michael; Hsu, Tsui-Ling; Chang, Chuan Fa; Wu, Chung‐Yi; Chen, Bo‐Rui; Huang, Hanwen; Liao, Kuo-Shiang; Chen, Chen-Chun; Chen, Chi‐Long; Yang, Shun-Min; Kuo, Chien-Nan; Chen, Peilin; Chiu, Ping-Tzu; Chen, I-Ju; Lai, Julie; Yu, Cheng-Der; Wong, Chi‐Huey

doi:10.1073/pnas.1816946116

Cited by 48 publications

(52 citation statements)

References 36 publications

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“…The glycolipid molecules SSEA-3 and SSEA-4 are known as embryonic stem cell markers that are identified in stem cells and cancer cells. 56,57 An expression of SSEA-4 was reported on porcine cardiac valves. 34 Zulli et al 35,58 reported that diseased human and rabbit blood vessels expressed for SSEA-4, and both SSEA-3 and SSEA-4, respectively, although normal blood vessels were negative for both SSEA-3 and SSEA-4.…”

Section: Discussionmentioning

confidence: 99%

Profiling natural serum antibodies of non‐human primates with a carbohydrate antigen microarray

Nanno

Sterner

Gildersleeve

et al. 2019

Xenotransplantation

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Background: Engineering of α-Galactosyltransferase gene-knockout pigs circumvented hyperacute rejection of pig organs after xenotransplantation in non-human primates. Overcoming this hurdle revealed the importance of non-α-Gal carbohydrate antigens in the immunobiology of acute humoral xenograft rejection. Methods: This study analyzed serum from seven naïve cynomolgus monkeys (blood type O/B/AB = 3/2/2) for the intensity of natural IgM and IgG signals using carbohydrate antigen microarray, which included historically reported α-Gal and non-α-Gal carbohydrate antigens with various modifications.Results: The median (range) of IgM and IgG signals were 12.71 (7.23-16.38) and 9.05 (7.23-15.90), respectively. The highest IgM and IgG signals with narrowest distribution were from mono-and disaccharides, followed by modified structures. Natural anti-α-Gal antibody signals were medium to high in IgM (11.2-15.9) and medium in IgG (8.5-11.6) spectra, and was highest with Lac core structure (Galα1-3Galβ1-4Glc, iGb3) and lowest with LacNAc core structure (Galα1-3Galβ1-4GlcNAc). Similar signal intensities (up to 15.8 in IgM and up to 11.8 in IgG) were observed for historically detected natural non-α-Gal antigens, which included Tn antigen, T antigen, GM2 glycolipid, and Sd a antigen. The hierarchical clustering analysis revealed the presence of clusters of anti-A antibodies and was capable of distinguishing between the blood group B and AB non-human primates. Conclusions:The results presented here provide the most comprehensive evaluation of natural antibodies present in cynomolgus monkeys. K E Y W O R D S carbohydrate antigen microarray, natural serum antibodies, non-human primates, xenotransplantation GM3(Gc)−Sp − 05 CT/Sda−Sp − 05

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Section: Discussionmentioning

confidence: 99%

Profiling natural serum antibodies of non‐human primates with a carbohydrate antigen microarray

Nanno

Sterner

Gildersleeve

et al. 2019

Xenotransplantation

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“…This results in upregulation of multidrug resistance gene 1 (MDR1) expression as well (Ghandadi et al, 2019). Cerebrosides levels rise in numerous cancer cell lines, as an effect of glucosyl-and galactosyltransferase overexpression (Chuang et al, 2019;Zhu et al, 2005).…”

Section: Glycosphingolipidsmentioning

confidence: 99%

Lipid composition of the cancer cell membrane

Szlasa

Zendran

Zalesińska

et al. 2020

J Bioenerg Biomembr

239

191

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Cancer cell possesses numerous adaptations to resist the immune system response and chemotherapy. One of the most significant properties of the neoplastic cells is the altered lipid metabolism, and consequently, the abnormal cell membrane composition. Like in the case of phosphatidylcholine, these changes result in the modulation of certain enzymes and accumulation of energetic material, which could be used for a higher proliferation rate. The changes are so prominent, that some lipids, such as phosphatidylserines, could even be considered as the cancer biomarkers. Additionally, some changes of biophysical properties of cell membranes lead to the higher resistance to chemotherapy, and finally to the disturbances in signalling pathways. Namely, the increased levels of certain lipids, like for instance phosphatidylserine, lead to the attenuation of the immune system response. Also, changes in lipid saturation prevent the cells from demanding conditions of the microenvironment. Particularly interesting is the significance of cell membrane cholesterol content in the modulation of metastasis. This review paper discusses the roles of each lipid type in cancer physiology. The review combined theoretical data with clinical studies to show novel therapeutic options concerning the modulation of cell membranes in oncology.

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“…Carbohydrate structures have been suggested to be associated with poor prognosis and reduced overall survival in breast cancer [18]. Globo H is one of the GalNAc‐containing glycans bound by the rBC2LCN; it was first identified in MCF‐7 cells and was found to play crucial roles in cancer stem cell properties and tumor progression in breast cancer [8–9,19–21]. Globo H expression is higher in MCF‐7 and T‐47D cells than in MDA‐MB‐231 [13,19].…”

Section: Discussionmentioning

confidence: 99%

rBC2LCN lectin as a potential probe of early‐stage HER2‐positive breast carcinoma

et al. 2020

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The recombinant N‐terminal domain of BC2L‐C lectin (rBC2LCN) is useful for detecting not only human pluripotent stem cells but also some cancers. However, the cancer types and stages that can be detected by rBC2LCN remain unclear. In this study, we identified the human breast carcinoma subtypes and stages that can be detected by rBC2LCN. Compared with rBC2LCN‐negative breast carcinoma cell lines, the rBC2LCN‐positive cells expressed higher levels of human epidermal growth factor receptor 2 (HER2) and epithelial marker genes. Importantly, rBC2LCN histochemical staining of human breast carcinoma tissues demonstrated the utility of rBC2LCN in detecting breast carcinoma types that express HER2 and have not spread much in the early phase of growth. We conclude that rBC2LCN may have potential as a detection probe and a drug delivery vehicle to identify and treat early‐stage HER2‐positive breast carcinoma.

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Signaling pathway of globo-series glycosphingolipids and β1,3-galactosyltransferase V (β3GalT5) in breast cancer

Cited by 48 publications

References 36 publications

Profiling natural serum antibodies of non‐human primates with a carbohydrate antigen microarray

Profiling natural serum antibodies of non‐human primates with a carbohydrate antigen microarray

Lipid composition of the cancer cell membrane

rBC2LCN lectin as a potential probe of early‐stage HER2‐positive breast carcinoma

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