Signaling interplay between PARP1 and ROS regulates stress-induced cell death and developmental changes in Dictyostelium discoideum

Mir, Hina; Rajawat, Jyotika; Vohra, Iqbal; Vaishnav, Jayvadan; Kadam, Ashlesha; Begum, Rasheedunnisa

doi:10.1016/j.yexcr.2020.112364

Cited by 7 publications

(1 citation statement)

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“…Besides HSP90AA1, additional EGT target genes may also contribute to the effect of treatment. For example, the other top‐ranked genes like HSPA2 [ 61 ] and PARP1 [ 62 ] are associated with oxidative stress. So future study in this area should be well designed to explore the EGT protective effects against oxidative stress.…”

Section: Discussionmentioning

confidence: 99%

Ergothioneine attenuates varicocele‐induced testicular damage by upregulating HSP90AA1 in rats

Chen

Zhou

Yang

et al. 2023

J Biochem & Molecular Tox

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This study investigates the therapeutic effect and the underlying mechanisms of ergothioneine (EGT) on the testicular damage caused by varicocele (VC) in vivo, in vitro, and in silico. This preclinical study combines a series of biological experiments and network pharmacology analyses. A total of 18 Sprague Dawley (SD) male rats were randomly and averagely divided into three groups: the sham-operated, VC model, and VC model with EGT treatment (VC + EGT) groups. The left renal vein of the VC model and the VC + EGT groups were half-ligated for 4 weeks. Meanwhile, the VC + EGT group was intragastrically administrated with EGT (10 mg/kg). GC1 and GC2 cells were exposed to H 2 O 2 with or without EGT treatment to re-verify the conclusion. The structure disorder of seminiferous tubules ameliorated the apoptosis decrease in the VC rats receiving EGT. EGT can also increase the sperm quality of the VC model rats (p < 0.05). The exposure to H 2 O 2 decreased proliferation and increased apoptosis of GC1 and GC2 cells, which was revisable by adding EGT to the plates (p < 0.05). The network pharmacology and molecular docking were conducted to explore the potential targets of EGT in VC, and HSP90AA1 was identified as the pivotal gene, which was validated by western blot, immunohistochemistry, and RT-qPCR both in vivo and in vitro (p < 0.05). Overall, EGT attenuates the testicular injury in the VC model both in vivo and in vitro by potentially potentiating the expression of HSP90AA1.

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Section: Discussionmentioning

confidence: 99%