Signaling Events Involved in Interleukin 27 (IL-27)-induced Proliferation of Human Naive CD4+ T Cells and B Cells

Charlot-Rabiega, Pascaline; Bardel, Emilie; Dietrich, Céline; Kastelein, Robert A.; Devergne, Odile

doi:10.1074/jbc.m111.221010

Cited by 35 publications

(29 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previous studies show that Myc could be induced by T cell antigen receptor (TCR) signal and various cytokines in T cells and is involved in cell activation and proliferation by regulating the genes responsible for metabolic reprogramming and cell cycle (31)(32)(33). In agreement with these findings, we found that inhibition of Myc blocked the TCR agonist-induced T cell activation and proliferation.…”

Section: Discussionsupporting

confidence: 81%

Down-regulation of microRNA-451a facilitates the activation and proliferation of CD4+ T cells by targeting Myc in patients with dilated cardiomyopathy

Zeng

Wang

et al. 2017

Journal of Biological Chemistry

View full text Add to dashboard Cite

show abstract

Section: Discussionsupporting

confidence: 81%

Down-regulation of microRNA-451a facilitates the activation and proliferation of CD4+ T cells by targeting Myc in patients with dilated cardiomyopathy

Zeng

Wang

et al. 2017

Journal of Biological Chemistry

View full text Add to dashboard Cite

show abstract

“…This elevated expression is reversed by IL-10 in a STAT3-dependent manner, revealing a negative feedback mechanism for IL-27 induced IL-10 production [12]. Furthermore, upregulation of both EBI3 as well as p28 occur during T cell activation with low but detectable levels in both naïve and memory CD4+ T cells and also in B cells [13].…”

Section: Expression Of Interleukin-27 and Its Receptorsmentioning

confidence: 96%

The biology of interleukin-27 reveals unique pro- and anti-inflammatory functions in immunity

Aparicio-Siegmund

Garbers

2015

Cytokine & Growth Factor Reviews

View full text Add to dashboard Cite

“…Human CD4 + T cells (purity .97%) or CD8 + T cells (purity .95%) were purified by negative magnetic cell isolation (Miltenyi Biotec) from PBMC of adult healthy donors (Etablissement Français du Sang, Paris, France), as previously described (18). Purified T cells were cultured (1 3 10 6 /ml) for 3-7 d in complete RPMI 1640 medium in the presence of IL-2 (20 U/ml; Roche), and either PHA (4 mg/ml; Sigma-Aldrich) or beads (ratio 1 bead per 2 cells) coated with CD2/CD3/CD28 Abs (T cell activation/expansion kit; Miltenyi Biotec).…”

Section: Methodsmentioning

confidence: 99%

A Soluble Form of IL-27Rα Is a Natural IL-27 Antagonist

Dietrich

Candon

Ruemmele

et al. 2014

The Journal of Immunology

Self Cite

View full text Add to dashboard Cite

IL-27 is a cytokine of the IL-12 family that plays a key role in the regulation of inflammatory and T cell responses. Its receptor is composed of IL-27Rα and gp130 and activates the STAT pathway. We show in this study, using an ELISA that we developed, that a naturally occurring soluble form of IL-27Rα (sIL-27Rα) is produced by human activated CD4+ and CD8+ T cells, B cells, myeloid cells, and various cell lines. sIL-27Rα is present at a mean concentration of 10,344 ± 1,274 pg/ml in the sera from healthy individuals. Biochemical studies showed that sIL-27Rα is released as two N-glycosylated variants of ∼90 and ∼70 kDa. In IL-27Rα–transfected COS7 cells, primary cells, and cell lines, production of sIL-27Rα is inhibited by the metalloprotease inhibitors GM6001 and TAPI-0. Importantly, natural sIL-27Rα binds rIL-27, inhibits IL-27 binding to its cell surface receptor, and is a potent inhibitor of IL-27 signaling, as shown by its ability to specifically block IL-27–mediated STAT activation, at low molar excess over IL-27. Also, we found that serum levels of sIL-27Rα were elevated in patients with Crohn’s disease, a Th1-mediated disease. These findings suggest that sIL-27Rα may play important immunoregulatory functions under normal and pathological conditions.

show abstract

Signaling Events Involved in Interleukin 27 (IL-27)-induced Proliferation of Human Naive CD4+ T Cells and B Cells

Cited by 35 publications

References 41 publications

Down-regulation of microRNA-451a facilitates the activation and proliferation of CD4+ T cells by targeting Myc in patients with dilated cardiomyopathy

Down-regulation of microRNA-451a facilitates the activation and proliferation of CD4+ T cells by targeting Myc in patients with dilated cardiomyopathy

The biology of interleukin-27 reveals unique pro- and anti-inflammatory functions in immunity

A Soluble Form of IL-27Rα Is a Natural IL-27 Antagonist

Contact Info

Product

Resources

About