Signal Transudation for Cecropin B Gene Expression in Adherent Hemocytes of the Silkworm, Bombyx mori (Lepidoptera: Bombycidae)

Shimabukuro, Michio; Xu, Jinhua; Sugiyama, Masao; Taniai, Kiyoko; Kadono-Okuda, Keiko; Kato, Yosuke; Yamamoto, Masanori; Chowdhury, Subrata; Choi, Kyung Su; Choi, Kee‐Hyun; Miyanoshita, Akihiro; Debnath, Chandan; Yamakawa, Minoru

doi:10.1303/aez.31.135

Cited by 15 publications

(8 citation statements)

References 12 publications

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“…PKA isotypes affect a myriad of cellular functions in invertebrates, including insects 32 , 33 , 34 . Haemocytes of the silkworm Bombyx mori require an unidentified PKA isotype to produce cecropins (antibacterial proteins) 35 , 36 . Based on the use of H‐89, an inhibitor of PKA isotypes, a type of PKA may limit G. mellonella haemocyte adhesion 8 .…”

Section: Discussionmentioning

confidence: 99%

Protein kinase A affects Galleria mellonella (Insecta: Lepidoptera) larval haemocyte non‐self responses

Brooks

Dunphy

2005

Immunol Cell Biol

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Summary We used the protein kinase A (PKA) specific activator Sp-8-Br-cAMPS and type I inhibitor Rp-8-BrcAMPS alone and in combination to define the role of PKA in the non-self responses of larval Galleria mellonella haemocytes in vitro and in vivo . Active PKA depressed haemocyte responses whereas PKA inhibition enhanced activities, including bacterial phagocytosis, the number of haemocytes with adherent bacteria, bacterial-induced haemocytic protein release and haemocyte adhesion to slides in vitro , as well as in vivo bacterial removal from the haemolymph. Non-attached haemocytes had more PKA activity than attached haemocytes; therefore, active PKA limited haemocyte response to foreign materials. We found that (i) PKA inhibitor alone induced non-self responses, including haemocyte protein discharge and lowered haemocyte counts in vivo , and induced nodulation; (ii) the enzyme activator produced effects opposite to those of the inhibitor; and (iii) together, the modulators offset each others' effects and influenced haemocyte lysate PKA activity. These findings establish PKA as a mediator of haemocytic non-self responses.

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Section: Discussionmentioning

confidence: 99%

Protein kinase A affects Galleria mellonella (Insecta: Lepidoptera) larval haemocyte non‐self responses

Brooks

Dunphy

2005

Immunol Cell Biol

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“…Divalent calcium, cyclic AMP and G-proteins lead to cecropin gene activation in Bombyx mori hemocytes (Choi et al, 1995). Such gene activation did not require tyrosine kinase or Ca 2+ /calmodulin-dependent kinase (Shimabukuro et al, 1996;Foukas et al, 1998). Peptidoglycan and lipopolysaccharides induced cecropin B synthesis by pathways involving protein kinase A and C but not by myosin light chain kinase .…”

Section: Introductionmentioning

confidence: 92%

Kinases, intracellular calcium, and apolipophorin‐III influence the adhesion of larval hemocytes of the lepidopterous insect, Galleria mellonella

Zakarian

Dunphy

Rau

et al. 2003

Arch Insect Biochem Physiol

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Based on the results from the use of selective inhibitors and activators, active protein kinase A, protein tyrosine kinase, and protein kinase C (PKC) isoforms decreased the adhesion of larval Galleria mellonella hemocytes to glass slides. The protein kinase A inhibitor at all concentrations increased granular cell adhesion only whereas protein tyrosine kinase elevated both granular and plasmatocyte attachment at the lowest concentration. Active, Ca(2+)- and lipid-dependent PKC isoforms limited plasmatocyte and granular cell adhesion whereas PKC that was inhibited by selected compounds (with differed modes of PKC inhibition) enhanced hemocyte attachment. The granular cells were more sensitive to the PKC inhibitors than were plasmatocytes. Phospholipase C and its diacylglyceride product were necessary to reduce hemocyte adhesion and maintain PKC activity. Extracellular Ca(2+), possibly transported through L-channels, was required for plasmatocyte attachment. In contrast, lowering the levels of cytosolic Ca(2+) was associated with decreased PKC activity and was required for hemocyte adhesion.

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“…17) Furthermore, factors involved in signal transduction for induction of antimicrobial peptide gene expression have also been analyzed in specific tissues. 17,19) Recently, two novel antimicrobial peptides have been identified from B. mori. First, an Enbocin gene was cloned using a partial cDNA fragment detected by differential hybridization as a probe.…”

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confidence: 99%

Expression of Antimicrobial Peptide Genes Encoding Enbocin and Gloverin Isoforms in the Silkworm,Bombyx mori

Kaneko

Furukawa

Tanaka

et al. 2007

Bioscience, Biotechnology, and Biochemistry

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Signal Transudation for Cecropin B Gene Expression in Adherent Hemocytes of the Silkworm, Bombyx mori (Lepidoptera: Bombycidae)

Cited by 15 publications

References 12 publications

Protein kinase A affects Galleria mellonella (Insecta: Lepidoptera) larval haemocyte non‐self responses

Protein kinase A affects Galleria mellonella (Insecta: Lepidoptera) larval haemocyte non‐self responses

Kinases, intracellular calcium, and apolipophorin‐III influence the adhesion of larval hemocytes of the lepidopterous insect, Galleria mellonella

Expression of Antimicrobial Peptide Genes Encoding Enbocin and Gloverin Isoforms in the Silkworm,Bombyx mori

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