Signal transduction pathway for oxidative burst and taxol production in suspension cultures of Taxus chinensis var. mairei induced by oligosaccharide from Fusarium oxysprum

Yang, Yuan; Li, Chun; Hu, Zongding; Wu, Jicang

doi:10.1016/s0141-0229(01)00406-9

Cited by 69 publications

(38 citation statements)

References 18 publications

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“…At this stage suspension cells were reached up to exponential growth phase, which is highly significant for the production of secondary metabolites. Furthermore, H2O2 has been reported to increase the secondary metabolite production in plants (Yuan et al, 2001(Yuan et al, , 2002. The present study further strengthens this view.…”

Section: Isolation and Quantification Of Steviosidesupporting

confidence: 88%

Antioxidant response of Stevia rebaudiana (Bertoni) Bertoni (Angiosperms; Asteraceae) during developing phase of suspension cell culture

Dwivedi¹,

Alam²,

Shekhawat³

2016

Plant Sci Today

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The present study established a protocol for suspension cell culture of Stevia rebaudiana and demonstrates the antioxidant enzymes activity during various developing phase of it. Higher concentration of Auxin and Cytokines (3.0 mg L -1 BAP and 4.0 mg L -1 NAA) with Ascorbic acid (1 mg L -1 ) considered as highly suitable growth regulator combination for growth and development of Stevia rebaudiana suspension culture. Maximum concentration of stevioside (~70 mg G -1 of dry weight of tissue) was obtained on 14 th day (exponential phase) which got reduced on 28 th day (apoptotic phase) upto ~21 mg G -1 of dry weight of tissue. The amount of MDA reduced initially up to stationary growth phase which showed the adaptation of suspension cells in the culture medium and culture environment. Reduction of Chlorophyll showed the enhancing phenolic content with progressive culture period, while enhanced amount of proline was indicating the generation of defense mechanism with depletion of nutrient (with increased culture duration). The amount of SOD got enhanced with elevating the concentration of H2O2 as well. The concentration of CAT enhanced upto stationary growth phase of suspension cell while APX concentration showed continuous inhibition up to apoptotic phase from exponential phase.

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Section: Isolation and Quantification Of Steviosidesupporting

confidence: 88%

Antioxidant response of Stevia rebaudiana (Bertoni) Bertoni (Angiosperms; Asteraceae) during developing phase of suspension cell culture

Dwivedi¹,

Alam²,

Shekhawat³

2016

Plant Sci Today

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“…The generation of NO is a hallmark of such responses to fungal elicitors (Delledonne et al, 1998;Durner et al, 1998). NO has multiple functions, e.g., the stimulation of seed germination (Sarath et al, 2006), formation of adventitious roots (Pagnussat et al, 2002(Pagnussat et al, , 2003Tewari et al, 2007) ROS production is an early event in the elicitationinduced manufacture of secondary metabolites in plant cell cultures through biotic and abiotic means (Yuan et al, 2001;Wu and Lin, 2003). NO also induces DNA fragmentation and cell death in Taxus haploid cultures (Pedroso et al, 2000).…”

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confidence: 99%

Nitric Oxide Elicitation Induces the Accumulation of Secondary Metabolites and Antioxidant Defense in Adventitious Roots ofEchinacea purpurea

Tewari

Hahn

et al. 2007

J. Plant Biol.

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Nitric oxide and reactive oxygen species are important signal molecules that play key roles in plant defense responses. We investigated the involvement of nitric oxide elicitation in the synthesis of secondary metabolites within the adventitious roots of Echinacea purpurea. When roots were treated with 100 I~M sodium nitroprusside (SNP), an exogenous nitric oxide producer, the accumulation of phenolics, flavonoids, and caffeic acid derivatives was enhanced. This level of SNP also induced an antioxidant defense, as indicated by increases in superoxide dismutase, ascorbate peroxidase, and ascorbic acid, along with decreases in hydrogen peroxide, lipid peroxidation, and dehydroascorbate/ascorbic acid. However, a higher concentration (250 ~M SNIP) acted as a pro-oxidant, thereby raising the levels of hydrogen peroxide, lipid peroxidation, and dehydroascorbate/ascorbic acid while diminishing ascorbic acid, ascorbate peroxidase, and the accumulation of secondary metabolites compared with our observations at 100 BM SNR Therefore, we conclude that eliciting E. purpurea adventitious roots with a concentration of 100 ~M SNP is beneficial to their accumulation of secondary metabolites. Keywords: antioxidant defense, bioreactor, Echinacea purpurea, elicitation, nitric oxide, secondary metabolitesThe production of secondary metabolites via plant cell/ organ cultures is an important area of investigation because of their commercial and medicinal importance. However, these compounds exist at only very low concentrations in plants, which limits their yields. Plant cells respond to various biotic and abiotic elicitors by activating a wide array of reactions [viz., ion fluxes across the plasma membrane, synthesis of reactive oxygen species (ROS), and phosphorylation and dephosphorylation of proteins]. These are all putative components of signal transduction pathways that lead to elicitor-induced defense responses, e.g., the activation of defense genes and hypersensitive cell death (Dietrich et al., 1990; N(~rnberger et al., 1994;Baker and Orlandi, 1995). It has been suggested that ROS alone cannot mediate a sufficient disease resistance response in plants, but in combination with nitric oxide (NO) can function synergistically to activate a stronger response (Wang and Wu, 2005). Therefore, NO is a diffusible, bioactive signalling molecule (Beligni and Lamattina, 2000;Neill et al., 2003; RomeroPuertas et al., 2004).The synthesis of secondary metabolites in plants is believed to be part of their defense response against biotic (pathogenic attack) and abiotic stresses. Applying the elicitors obtained from microorganisms (fungi and bacteria) is an effective strategy for improving the productivity of seconclary metabolites in plant cell/organ cultures (Roberts and Shuler, 1997). The generation of NO is a hallmark of such responses to fungal elicitors (Delledonne et al., 1998;Durner et al., 1998). NO has multiple functions, e.g., the stimulation of seed germination (Sarath et al., 2006), formation of adventitious roots (Pagnussat et al....

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“…The reactive oxygen intermediates (ROI) such as H 2 O 2 generated by oxidative burst are considered to be important signaling molecules in plants. H 2 O 2 has been reported to be implicated in the cross-linking of cell wall proteins (Bradley et al 1992), in inducing pathogensisrelated (PR-1) gene expression (Chamnongpol et al 1998), in plant cell death process (Levine et al 1994), in the induction of hypersensitive reaction (HR) (Baker et al 1993), and in mediating the production of plant secondary metabolites (Xu et al 2008;Yuan et al 2001). The results of this work show that H 2 O 2 levels of the cells treated with PB90 are much higher than the control cells, suggesting that PB90 may trigger H 2 O 2 generation of the cells.…”

Section: Discussionmentioning

confidence: 99%

Involvement of NADPH oxidase-mediated H2O2 signaling in PB90-induced hypericin accumulation in Hypericum perforatum cells

Sheng

Wang

et al. 2010

Plant Cell Tiss Organ Cult

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PB90 is a novel protein elicitor isolated from Phytophthora boehmeriae. Here, we report that treatment of PB90 stimulates hypericin production and hydrogen peroxide (H 2 O 2 ) generation in Hypericum perforatum L. cells and demonstrate that H 2 O 2 is essential for PB90-induced hypericin production. To further study the source of PB90-triggered H 2 O 2 , we have investigated activities of plasma membrane NADPH oxidase in Hypericum perforatum L. cells subjected to PB90 treatment. It is revealed that treatment of the cells with PB90 significantly increases NADPH oxidase activity. NADPH oxidase inhibitors suppress not only the PB90-stimulated NADPH oxidase activity but also the PB90-triggered H 2 O 2 generation and PB90-induced hypericin production, showing that NADPH oxidase is involved in PB90-triggered H 2 O 2 generation and hypericin production. Moreover, the suppression of NADPH oxidase inhibitors on PB90-induced hypericin production can be reversed by H 2 O 2 , although H 2 O 2 per se has no effects on hypericin production of the cells. Together, the data demonstrate that PB90 may induce hypericin production of H. perforatum cells through the NADPH oxidase-mediated H 2 O 2 signaling pathway.

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Signal transduction pathway for oxidative burst and taxol production in suspension cultures of Taxus chinensis var. mairei induced by oligosaccharide from Fusarium oxysprum

Cited by 69 publications

References 18 publications

Antioxidant response of Stevia rebaudiana (Bertoni) Bertoni (Angiosperms; Asteraceae) during developing phase of suspension cell culture

Antioxidant response of Stevia rebaudiana (Bertoni) Bertoni (Angiosperms; Asteraceae) during developing phase of suspension cell culture

Nitric Oxide Elicitation Induces the Accumulation of Secondary Metabolites and Antioxidant Defense in Adventitious Roots ofEchinacea purpurea

Involvement of NADPH oxidase-mediated H2O2 signaling in PB90-induced hypericin accumulation in Hypericum perforatum cells

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