Signal transduction in yeast mating: Receptors, transcription factors, and the kinase connection

Sprague, G

doi:10.1016/0168-9525(91)90218-f

Cited by 75 publications

(24 citation statements)

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“…Significant progress towards understanding some of the basic processes that mediate cell responses to different stimuli has been made in recent years. Many studies carried out with Saccharomyces cerevisiae have documented, for example, the signal transduction cascade activated in response to the mating pheromone, which involves a number of sequentially acting protein kinases (1,20,41,42,46,47,64). More recently, two other signal transduction cascades, namely, the high-osmolarity glycerol response pathway (6) and the PKC1-mediated signal transduction pathway (20), have been identified.…”

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confidence: 99%

Functional Characterization of the MKC1 Gene of Candida albicans, Which Encodes a Mitogen-Activated Protein Kinase Homolog Related to Cell Integrity

Navarro‐García

Sánchez

Pla

et al. 1995

Molecular and Cellular Biology

182

154

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Mitogen-activated protein (MAP) kinases represent a group of serine/threonine protein kinases playing a central role in signal transduction processes in eukaryotic cells. Using a strategy based on the complementation of the thermosensitive autolytic phenotype of slt2 null mutants, we have isolated a Candida albicans homolog of Saccharomyces cerevisiae MAP kinase gene SLT2 (MPK1), which is involved in the recently outlined PKC1-controlled signalling pathway. The isolated gene, named MKC1 (MAP kinase from C. albicans), coded for a putative protein, Mkc1p, of 58,320 Da that displayed all the characteristic domains of MAP kinases and was 55% identical to S. cerevisiae Slt2p (Mpk1p). The MKC1 gene was deleted in a diploid Candida strain, and heterozygous and homozygous strains, in both Ura ؉ and Ura ؊ backgrounds, were obtained to facilitate the analysis of the function of the gene. Deletion of the two alleles of the MKC1 gene gave rise to viable cells that grew at 28 and 37؇C but, nevertheless, displayed a variety of phenotypic traits under more stringent conditions. These included a low growth yield and a loss of viability in cultures grown at 42؇C, a high sensitivity to thermal shocks at 55؇C, an enhanced susceptibility to caffeine that was osmotically remediable, and the formation of a weak cell wall with a very low resistance to complex lytic enzyme preparations. The analysis of the functions downstream of the MKC1 gene should contribute to understanding of the connection of growth and morphogenesis in pathogenic fungi.

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confidence: 99%

Functional Characterization of the MKC1 Gene of Candida albicans, Which Encodes a Mitogen-Activated Protein Kinase Homolog Related to Cell Integrity

Navarro‐García

Sánchez

Pla

et al. 1995

Molecular and Cellular Biology

182

154

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“…This difference arises from cell type-specific transcription; ~ cells uniquely express a-specific genes, and a cells uniquely express a-specific genes. In addition, a set of haploid-specific genes is expressed in both a and cells but not in a/R cells (for review, see Herskowitz 1989;Sprague 1990;Dolan and Fields 1991 ). Genetically, a and et cells differ at only a single locus, MAT.…”

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confidence: 99%

“…In addition, many haploid-specific genes are transcriptionally induced in response to the appropriate pheromone. The signal pathway that mediates these changes in gene expression appears to be identical in both haploid cell types and includes a heterotrimeric G protein, the STE5 protein, five proteins with homology to protein kinases (STE7, STEll, STE20, FUS3, and KSS1), and a transcriptional activator (STE12) (for review, see Marsh et al 1991;Dolan and Fields 1991;Sprague 1991;Kurjan 1992; see also Leberer et al 1992;Ramer et al 1993). STE12 binds to the pheromone response element (PRE), the DNA sequence responsible for a-factor induction of a-specific genes and for a-or a-factor induction of haploid-specific genes (Dolan et al 1989;Errede and Ammerer 1989).…”

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Coupling of cell identity to signal response in yeast: interaction between the alpha 1 and STE12 proteins.

Yuan¹,

Stroke²,

Fields³

1993

Genes Dev.

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In Saccharomyces cerevisiae, the STE12 protein mediates transcriptional induction of cell type-specific genes in response to pheromones. STE12 binds in vitro to the pheromone response elements (PREs) present in the control region of a-specific genes. STE12 is also required for transcription of o~-specific genes, but there is no evidence that it binds directly to these genes. Instead, the MAToL-encoded protein o~l and the MCM1 product bind to the DNA element that is responsible for ,v-specific and a-factor-inducible expression. To explore the role of STE12 in the pheromone induction of ,v-specific genes, we cloned STE12 and MATal homologs from the related yeast Kluyveromyces lactis. The K. lactis STE12 protein did not cooperate with the S. cerevisiae od protein to promote the overall mating process or the induction of transcription of an s-specific gene. However, introduction of both K. lactis STE12 along with K. lactis od did restore mating, suggesting that an interaction between STE12 and ~1 is important for s-specific gene activation. We also show that bacterially expressed STE12 and r are able to form a complex in vitro. Thus, we demonstrate a coupling in ~ cells between a protein functioning in cell identity, ~1, with a protein responsive to the pheromone-induced signal STE12.

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“…A heterotrimeric G protein then transduces the signal to cytoplasmic components of the signal pathway (for a review, see reference 1). The intracellular pathway transmits the signal to the nucleus, where it causes transcription to be induced for a variety of genes whose products execute the events of mating (for a review, see reference 41). Additionally, the pheromone-induced signal inhibits progression of the cell cycle at the G1 phase by regulating the activity of G1-specific cyclins (for a review, see reference 31).…”

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Pheromone-induced signal transduction in Saccharomyces cerevisiae requires the sequential function of three protein kinases.

Zhou¹,

Gartner²,

Cade³

et al. 1993

Mol. Cell. Biol.

135

107

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Signal transduction in yeast mating: Receptors, transcription factors, and the kinase connection

Cited by 75 publications

References 0 publications

Functional Characterization of the MKC1 Gene of Candida albicans, Which Encodes a Mitogen-Activated Protein Kinase Homolog Related to Cell Integrity

Functional Characterization of the MKC1 Gene of Candida albicans, Which Encodes a Mitogen-Activated Protein Kinase Homolog Related to Cell Integrity

Coupling of cell identity to signal response in yeast: interaction between the alpha 1 and STE12 proteins.

Pheromone-induced signal transduction in Saccharomyces cerevisiae requires the sequential function of three protein kinases.

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