Signal transduction events in human peripheral blood mononuclear cells stimulated by schistosoma mansoni antigens

Almeida, Célia A; Leite, M. Fátima; Góes, Alfredo M.

doi:10.1016/s0198-8859(01)00302-0

Cited by 6 publications

(5 citation statements)

References 32 publications

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“…Almeida et al (2001) [44] have shown that after SWAP stimulation, the phosphorylation levels of Lck and Shc were more pronounced in the SWAP- than in the SEA-stimulated PBMCs. These data suggest that SEA and SWAP induce proliferation of lymphocytes both selectively and separately.…”

Section: Discussionmentioning

confidence: 99%

Cytokine profile, proliferation and phosphorylation of ERK1/2 and Akt in circulating mononuclear cells from individuals during the chronic intestinal phase of Schistosomiasis mansoni infection

et al. 2012

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BackgroundThe immune response to Schistosoma mansoni is characterized by a granulomatous reaction around the parasite eggs that are trapped in the host liver, and this reaction modulates the immune response during the chronic phase of the disease. The typical peripheral blood mononuclear cell (PBMC) response of patients during the chronic intestinal phase of infection is characterized by a decreased response to an S. mansoni soluble egg antigen. To obtain a greater understanding of Schistosoma infections, this study investigated the effects of the soluble egg antigen (SEA) and soluble adult worm antigen (SWAP) of S. mansoni on cellular proliferation, cytokine production, and ERK1/2 and Akt phosphorylation in PBMCs from infected (XTO) and egg-negative (NI) individuals living in the same endemic area.MethodsThe activation status was evaluated by cell immunophenotypic staining (cytometry). The cell proliferation assay was by CFSE method. Cytokine detection assay (Th1 and Th2) was by Cytometric Bead and Array phosphorylation status was by ELISA.ResultsThe XTO, NI and BD (blood donor) individuals from an area not endemic for schistosomiasis were compared. The CD4+ T lymphocyte proliferation rate was lower in the XTO group, but not the NI group, after SEA stimulation compared to the BD group. The CD8+ T cell proliferation rate was lower in the XTO group in the unstimulated cultures and after both SEA and SWAP stimulation compared to the BD group. Cytokine analysis after either SEA or SWAP stimulation showed a balanced cytokine pattern in the XTO and NI groups. ERK1/2 and Akt phosphorylation were only marginally detected in all groups; however, a decrease in ERK 1/2 phosphorylation was observed in the SWAP-stimulated XTO group compared to both the NI and BD groups.ConclusionsThe data indicate that SEA-stimulated CD4+ T cells from infected patients have a lower proliferation rate than the same cells from the NI group. Furthermore, we observed that SWAP stimulation influences ERK1/2 phosphorylation in the XTO group.

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Section: Discussionmentioning

confidence: 99%

Cytokine profile, proliferation and phosphorylation of ERK1/2 and Akt in circulating mononuclear cells from individuals during the chronic intestinal phase of Schistosomiasis mansoni infection

et al. 2012

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“…The different pharmacological actions of oxamniquine and praziquantel did not affect the magnitude or type of changes they induced as shown in Table 1b, suggesting that the changes are due to increased antigen presented to the immune system and possible increased immune reactivity to parasite antigens, and not the different modes of action of the drugs. As praziquantel acts rapidly (within hours) and oxamniquine can take several days to be effective (31), this may have resulted in temporal differences in tegumental damage to the adult worms. In this study, these differences did not translate into quantitative differences in the immune responses observed following treatment.…”

Section: Discussionmentioning

confidence: 99%

Chemotherapy‐induced, age‐related changes in antischistosome antibody responses

Mutapi

Hagan

Woolhouse

et al. 2003

Parasite Immunology

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Humoral responses directed against Schistosoma mansoni soluble egg antigen were studied in Zimbabwean children before and after treatment with either praziquantel (PZQ) or oxamniquine (OXAM). Treated children showed a significant increase in the proportion producing IgE and IgG3 and in mean levels of IgE, IgM, IgG3 six weeks post-treatment. At 18 weeks post-treatment, the proportion of treated children producing IgA, IgE, and IgG3 increased while the proportion producing IgG1 and IgG4 decreased. Mean levels of IgA, IgE, and IgG3 were higher than pre-treatment levels while levels of IgG1, IgG4 and IgM were lower. Statistical analyses showed that the magnitude of change in levels of IgE, IgM and IgG3 at 6 weeks post-treatment and of IgE, IgG3 and IgG4 at 18 weeks post-treatment was significantly greater in treated compared to untreated children, and there were no significant differences in immune responses between children treated with praziquantel and those treated with oxamniquine. The magnitude of change in IgE at 6 and 18 weeks, IgM at 6 weeks and IgG3 at 18 weeks post-treatment were significantly associated with age in treated but not in untreated children, with the change being greater in younger children. This suggests that treatment induced a change in the age-antibody relationship for these isotypes, and that the age-antibody relationship is not robust to chemotherapy. Pre-treatment infection levels were significantly associated (positive correlation) with the magnitude of change for IgE and IgG3 at 18 weeks post-treatment. Taken together, these results indicate that the age-antibody relationship observed in these children is due, at least in part, to cumulative host experience of parasite antigens and not host age alone.

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“…Several reports attempted to reveal the underlying mechanisms of the severity of co-infection. The majority of these have focused on the role of growth factor rich soluble egg antigen (SEA), in contrary to adult worm soluble antigens, on angiogenic processes and granuloma formation mediated by peripheral blood mononuclear cell (PBMC) [ 11 ] and endothelial cell proliferation [ 12 ] through distinct intracellular signaling pathway[ 13 ]. Such mitogenic activity of SEA was shown to be cell specific, dose dependent and involves up regulation of cell cycle engine promoting genes [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Soluble egg antigen of Schistosoma Haematobiuminduces HCV replication in PBMC from patients with chronic HCV infection

et al. 2006

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Signal transduction events in human peripheral blood mononuclear cells stimulated by schistosoma mansoni antigens

Cited by 6 publications

References 32 publications

Cytokine profile, proliferation and phosphorylation of ERK1/2 and Akt in circulating mononuclear cells from individuals during the chronic intestinal phase of Schistosomiasis mansoni infection

Cytokine profile, proliferation and phosphorylation of ERK1/2 and Akt in circulating mononuclear cells from individuals during the chronic intestinal phase of Schistosomiasis mansoni infection

Chemotherapy‐induced, age‐related changes in antischistosome antibody responses

Soluble egg antigen of Schistosoma Haematobiuminduces HCV replication in PBMC from patients with chronic HCV infection

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