Signal to noise analysis of multiple color fluorescence imaging microscopy

Garini, Yuval; Â, Gil; Bar‐Am, Irit; Cabib, Dario; Katzir, Nir

doi:10.1002/(sici)1097-0320(19990301)35:3<214::aid-cyto4>3.0.co;2-d

Cited by 59 publications

(91 citation statements)

References 14 publications

(9 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This classification procedure is the basis for Spectral Karyotyping and for definitive chromosome recognition. 15 The DAPI counterstained image of the same metaphase can be band-enhanced and displayed side by side with the spectral image which greatly facilitates the integration of chromosome band information within the analysis.…”

Section: Spectral Karyotyping (Sky™)mentioning

confidence: 99%

Recombinant balanced and unbalanced translocations as a consequence of a balanced complex chromosomal rearrangement involving eight breakpoints in four chromosomes

et al. 1999

View full text Add to dashboard Cite

We report on a family with a balanced complex chromosomal rearrangement (CCR) involving eight breakpoints between chromosomes 6, 7, 18, and 21 in the father. All three sons inherited one derivative chromosome from the father and in addition each inherited a different recombinant chromosome resulting in a partial trisomy 6q in the first, an apparently balanced karyotype in the second, and a partial trisomy 7q in the third son. Fluorescence in situ hybridisation (FISH) and microsatellite analysis were essential for the identification of the breakpoints. In addition, the results were confirmed by a 24-colour FISH experiment using the spectral karyotyping (SKY™) system. Paternal origin of the de novo CCR in the father was demonstrated for the first time by haplotype analysis. This is the second report of a CCR leading to simpler but unbalanced translocations in offspring as a consequence of recombination during gametogenesis, and the first report of a family case of CCR exhibiting as many as eight breakpoints in the transmitting carrier. The initial prediction that viable offspring would be quite unlikely had to be revised after the birth of three children. Genetic counselling of carriers of balanced complex rearrangements has to consider a higher probability for unbalanced recombinations than has been so far commonly assumed.

show abstract

Section: Spectral Karyotyping (Sky™)mentioning

confidence: 99%

Recombinant balanced and unbalanced translocations as a consequence of a balanced complex chromosomal rearrangement involving eight breakpoints in four chromosomes

et al. 1999

View full text Add to dashboard Cite

show abstract

“…The intensity information was converted into a classification of cells as target or nontarget cells using two commonly used methods: using a fixed threshold above the background level (26) and using a fixed signal-to-noise ratio (14,23,25). To set thresholds, five FISH images from each sample were analyzed manually, and the percentage of target cells based on the number of total cells was calculated for each image.…”

Section: Resultsmentioning

confidence: 99%

“…As noted above, fluorescence intensity varies between experiments, so the threshold is often set manually for each experiment. Another common approach is to set a fixed signal-to-noise ratio (14,23,25). Pernthaler et al used a fixed signal-to-noise ratio and defined the threshold as the mean background gray value of a FISH gray image multiplied by a signal-to-noise factor (25).…”

mentioning

confidence: 99%

Automated Image Analysis for Quantitative Fluorescence In Situ Hybridization with Environmental Samples

Zhou

Pons

Raskin

et al. 2007

Appl Environ Microbiol

View full text Add to dashboard Cite

“…They depend on the specific spectra that have to be separated and in the case of fluorescence, the complexity is even higher. The fluorochromes have to be excited at certain spectral ranges, which limits the spectral ranges that are available to detection (24). Zimmermann et al (18) have formulated the problem for the case of live-cell spectral imaging which introduces even stronger restrictions on the acquisition times.…”

Section: Spectral Imaging Challenge: Informationmentioning

confidence: 99%

Spectral imaging: Principles and applications

2006

View full text Add to dashboard Cite

Background: Spectral imaging extends the capabilities of biological and clinical studies to simultaneously study multiple features such as organelles and proteins qualitatively and quantitatively. Spectral imaging combines two well-known scientific methodologies, namely spectroscopy and imaging, to provide a new advantageous tool. The need to measure the spectrum at each point of the image requires combining dispersive optics with the more common imaging equipment, and introduces constrains as well. Methods and Results: The principles of spectral imaging and a few representative applications are described. Spectral imaging analysis is necessary because the complex data structure cannot be analyzed visually. A few of the

show abstract

Signal to noise analysis of multiple color fluorescence imaging microscopy

Cited by 59 publications

References 14 publications

Recombinant balanced and unbalanced translocations as a consequence of a balanced complex chromosomal rearrangement involving eight breakpoints in four chromosomes

Recombinant balanced and unbalanced translocations as a consequence of a balanced complex chromosomal rearrangement involving eight breakpoints in four chromosomes

Automated Image Analysis for Quantitative Fluorescence In Situ Hybridization with Environmental Samples

Spectral imaging: Principles and applications

Contact Info

Product

Resources

About