2010
DOI: 10.1590/s1413-86702010000200006 View full text |Buy / Rent full text
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Abstract: Background: Polymerase chain reaction (PCR) methods play an essential role in providing data related to diagnosis, monitoring and treatment of hepatitis C virus (HCV) infection. EIA results are reported as "reactive" or "non reactive" and EIA S/CO ratio may also be reported as "high" or "low." This study aimed to evaluate the performance of a real-time RT-PCR and assess whether there is relationship between S/CO and PCR results. Study Design and Methods: Sera from blood donors were analyzed by Enzyme-Linked Im… Show more

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“…Amplification was performed using a 7500 Real-Time Sequence Detection System (SDS; ABI Prism 7500; Applied Biosystems, CA). RT-PCR product accumulation was monitored using a TaqMan probe 3940.…”
Section: Methodsmentioning
“…Dilutions of this standard were tested with a total of 24 replicates for each point dilution to determine the positive cut-off point or lower limit of detection. RT-PCR product accumulation was monitored using a TaqMan probe 3940.…”
Section: Methodsmentioning
“…The increasingly sophisticated methods of diagnosing HCV infection have a direct impact on patient management and the use of more sensitive and specific assays is essential for an efficient diagnosis of HCV infection (Albertoni et al, 2010). Several seroprevalence studies have indicated that S/Co ratios could be used to accurately predict a positive status in conjunction with a confirmatory test Centers for Disease Control and Prevention, 2003;Dos Santos et al, 1999;Tobler et al, 2000).…”
Section: Discussionmentioning
“…Several seroprevalence studies have indicated that S/Co ratios could be used to accurately predict a positive status in conjunction with a confirmatory test Centers for Disease Control and Prevention, 2003;Dos Santos et al, 1999;Tobler et al, 2000). Although the majority of the seroprevalence studies reported were performed using the commercially available EIA test (Centers for Disease Control and Prevention, 2003;Dos Santos et al, 1999;Tobler et al, 2000;Albertoni et al, 2010), in the present study we utilized VITROS anti-HCV assay, whose performance was evaluated in some previous published studies. As reported by Ismail et al (2004), we also found, in the S/Co range of between 1 and 8, EIA negative/CIA positive samples.…”
Section: Discussionmentioning
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