Signal percolation through plants and the shape of the calcium signature

Plieth, Christoph

doi:10.4161/psb.5.4.10717

Cited by 9 publications

(11 citation statements)

References 33 publications

(34 reference statements)

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“…Instead, their response is similar with eATP. These data, in agreement with previous reports [38], [39], indicate that the kinetic analysis of an averaged response does not necessarily reflect the response of a single cell.…”

Section: Resultssupporting

confidence: 92%

“…Moreover, the fast acquisition rate (typically 2–10 Hz for a single plane), enables one to obtain an easy visualization of Ca 2+ signal percolation in plants (see also Movies S4, S5, S6, S7) [39]. In order to demonstrate the SPIM ability to perform 3D analysis we treated primary root tips of Arabidopsis seedlings with 0.1 mM eATP, acquiring adjacent image planes (typically 10 planes, with 5–10 µm steps, every 5–8 s), in the course of the stimulus.…”

Section: Resultsmentioning

confidence: 99%

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Calcium Dynamics in Root Cells of Arabidopsis thaliana Visualized with Selective Plane Illumination Microscopy

et al. 2013

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Selective Plane Illumination Microscopy (SPIM) is an imaging technique particularly suited for long term in-vivo analysis of transparent specimens, able to visualize small organs or entire organisms, at cellular and eventually even subcellular resolution. Here we report the application of SPIM in Calcium imaging based on Förster Resonance Energy Transfer (FRET). Transgenic Arabidopsis plants expressing the genetically encoded-FRET-based Ca2+ probe Cameleon, in the cytosol or nucleus, were used to demonstrate that SPIM enables ratiometric fluorescence imaging at high spatial and temporal resolution, both at tissue and single cell level. The SPIM-FRET technique enabled us to follow nuclear and cytosolic Ca2+ dynamics in Arabidopsis root tip cells, deep inside the organ, in response to different stimuli. A relevant physiological phenomenon, namely Ca2+ signal percolation, predicted in previous studies, has been directly visualized.

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Section: Resultssupporting

confidence: 92%

Section: Resultsmentioning

confidence: 99%

Calcium Dynamics in Root Cells of Arabidopsis thaliana Visualized with Selective Plane Illumination Microscopy

et al. 2013

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“…To study in more detail the mechanisms underlying the relation between the local and global signatures, Plieth [ 180 ] proposes to model the propagation of Ca 2+ signals between cells. The excitation of a cell results in a calcium spike described by a sum of exponentials, and can be propagated to neighbour cells according to cellular automata type rules.…”

Section: Calcium and Specificitymentioning

confidence: 99%

“…Cellular automata type models commonly refer to binary behaviours. In Plieth’s model [ 180 ] a cell is either excited or not, and all the individual excitations look the same. Going beyond a binary rule could help clarify how the different geometries may affect the transmission of the specific calcium kinetics from the place where the plant was stimulated to a distant cell.…”

Section: Calcium and Specificitymentioning

confidence: 99%

Towards the Physics of Calcium Signalling in Plants

Martins

Evans

Woolfenden

et al. 2013

Plants

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Calcium is an abundant element with a wide variety of important roles within cells. Calcium ions are inter- and intra-cellular messengers that are involved in numerous signalling pathways. Fluctuating compartment-specific calcium ion concentrations can lead to localised and even plant-wide oscillations that can regulate downstream events. Understanding the mechanisms that give rise to these complex patterns that vary both in space and time can be challenging, even in cases for which individual components have been identified. Taking a systems biology approach, mathematical and computational techniques can be employed to produce models that recapitulate experimental observations and capture our current understanding of the system. Useful models make novel predictions that can be investigated and falsified experimentally. This review brings together recent work on the modelling of calcium signalling in plants, from the scale of ion channels through to plant-wide responses to external stimuli. Some in silico results that have informed later experiments are highlighted.

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“…This requires cell-to-cell communication and a systemic spread of signals. 41 Here, the CaDIRA scenario depicted above (Fig. 1) can put ideas across and provide explanations: With a local stimulation (e.g., attack of a phytophagous insect or local heat treatment) few cells around the site of stimulation undergo CaDIRA and switch to an 'excited state'.…”

Section: Peroxidases Involved In Molecular Signalingmentioning

confidence: 99%