Signal-on electrogenerated chemiluminescence biosensor for ultrasensitive detection of microRNA-21 based on isothermal strand-displacement polymerase reaction and bridge DNA-gold nanoparticles

Cui, Aiping; Zhang, Jianwei; Bai, Wanqiao; Sun, Hongwei; Bao, Lin; Ma, Fen; Li, Yan

doi:10.1016/j.bios.2019.111664

Cited by 38 publications

(21 citation statements)

References 55 publications

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“…This result is consistent with previous studies on the expression levels of miR-21 in different cancer cells. [23][24][25] The detection of miR-21 extracted from the two kinds of cells with a concentration of 10 4 cells mL À1 showed that the degree of fluorescence enhancement caused by miR-21 in MCF-7 cells was significantly higher than that in HeLa cells, which was consistent with the above conclusion.…”

supporting

confidence: 83%

A wheel-like DNA nanosensor with background correction for analysis of miRNA-21 in living cells

Deng

et al. 2022

Chem. Commun.

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supporting

confidence: 83%

A wheel-like DNA nanosensor with background correction for analysis of miRNA-21 in living cells

Deng

et al. 2022

Chem. Commun.

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“…For improving the detection limit of the ECL biosensor, Cui et al [ 101 ] amplified indirect signals with signal amplification techniques. This ECL biosensor was based on the double signal amplification of an isotherstranded display polymerase reaction (ISDPR) and bridge DNA–AuNPs nanocomposites.…”

Section: Biosensormentioning

confidence: 99%

A Review of Biosensors for Detecting Tumor Markers in Breast Cancer

Hong

Sun

et al. 2022

Life

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Breast cancer has the highest cancer incidence rate in women. Early screening of breast cancer can effectively improve the treatment effect of patients. However, the main diagnostic techniques available for the detection of breast cancer require the corresponding equipment, professional practitioners, and expert analysis, and the detection cost is high. Tumor markers are a kind of active substance that can indicate the existence and growth of the tumor. The detection of tumor markers can effectively assist the diagnosis and treatment of breast cancer. The conventional detection methods of tumor markers have some shortcomings, such as insufficient sensitivity, expensive equipment, and complicated operations. Compared with these methods, biosensors have the advantages of high sensitivity, simple operation, low equipment cost, and can quantitatively detect all kinds of tumor markers. This review summarizes the biosensors (2013–2021) for the detection of breast cancer biomarkers. Firstly, the various reported tumor markers of breast cancer are introduced. Then, the development of biosensors designed for the sensitive, stable, and selective recognition of breast cancer biomarkers was systematically discussed, with special attention to the main clinical biomarkers, such as human epidermal growth factor receptor-2 (HER2) and estrogen receptor (ER). Finally, the opportunities and challenges of developing efficient biosensors in breast cancer diagnosis and treatment are discussed.

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“…Cyclic voltammograms (CVs) and electrochemical impedance spectroscopy (EIS) were carried out to analyze every step of the biosensor construction (shown in Figure A,B). The process begins with a finely polished GCE, which displays a definite redox peak of [Fe(CN) 6 ] −3/–4 and a small EIS semicircle due to its thoroughly cleaned surface (curve a). , When AuNPs were assembled onto the GCE surface, the redox intensity rose slightly, and the semicircle was reduced to be negligible, indicating that the electrical conductivity was enhanced by AuNPs (curve b). , Subsequent modification of the versatile probe contributed to significant changes in CV and EIS measurements. As electron transfer was obstructed by the repulsive force of negatively charged DNA on [Fe(CN) 6 ] −3 / –4 , the resistance (Ret) greatly increased, and the CV current significantly decreased (curve c) .…”

Section: Resultsmentioning

confidence: 96%

“…41,42 When AuNPs were assembled onto the GCE surface, the redox intensity rose slightly, and the semicircle was reduced to be negligible, indicating that the electrical conductivity was enhanced by AuNPs (curve b). 43,44 Subsequent modification of the versatile probe contributed to significant changes in CV and EIS measurements. As electron transfer was obstructed by the repulsive force of negatively charged DNA on [Fe-(CN) 6 ] −3 / −4 , the resistance (Ret) greatly increased, and the CV current significantly decreased (curve c).…”

Section: ■ Results and Discussionmentioning

confidence: 99%

A Multitargeted Electrochemiluminescent Biosensor Coupling DNAzyme with Cascading Amplification for Analyzing Myocardial miRNAs

et al. 2021

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Several circulating miRNAs are associated with the pathogenic process of acute myocardial infarction (AMI). Thus, analyzing myocardial miRNAs in the circulatory system is important for the diagnosis and treatment of AMI, especially for early-stage diagnosis. Based on the characteristics of myocardial miRNAs, an ultrasensitive and multitargeted electrochemiluminescence (ECL) sensing platform was developed with a versatile probe that can couple DNAzyme with hybridization chain reaction amplification. The target miRNA and auxiliary chains form a circular unit that shears the versatile probe hairpin, and the products subsequently trigger cascading amplification; a long strand of dsDNA is then generated with many C-rich sequences that can undergo in situ reductions to generate ECL luminophore silver clusters. Using this strategy, three myocardial miRNAs are successfully detected with a detection limit as low as 29.6 aM (S/N = 3). Notably, our method can detect myocardial miRNA groups composed of multiple related circulating miRNAs with high selectivity over interfering miRNAs in blood. This is extremely important for solving the problem of diverse and low abundance of infarct-associated miRNAs. Our strategy pioneers a new idea of miRNA detection, and given its versatility and sensitivity, it is promising for the diagnosis of multigene-regulated cardiovascular diseases.

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Signal-on electrogenerated chemiluminescence biosensor for ultrasensitive detection of microRNA-21 based on isothermal strand-displacement polymerase reaction and bridge DNA-gold nanoparticles

Cited by 38 publications

References 55 publications

A wheel-like DNA nanosensor with background correction for analysis of miRNA-21 in living cells

A wheel-like DNA nanosensor with background correction for analysis of miRNA-21 in living cells

A Review of Biosensors for Detecting Tumor Markers in Breast Cancer

A Multitargeted Electrochemiluminescent Biosensor Coupling DNAzyme with Cascading Amplification for Analyzing Myocardial miRNAs

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