Signal-off electrochemiluminescence immunosensor based on Mn-Eumelanin coordination nanoparticles quenching PtCo-CuFe2O4-reduced graphene oxide enhanced luminol

Li, Xiaojian; Du, Yu; Xu, Peng; Li, Yueyun; Ren, Xiang; Ma, Hongmin; Wang, Huan; Wei, Qin; Ju, Huangxian

doi:10.1016/j.snb.2020.128702

Cited by 12 publications

(6 citation statements)

References 29 publications

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“…Figure S4 showed that the zeta-potential of Ti 3 C 2 T x MXene@TiO 2 –MoS 2 hybrids and Ab 1 were −30.2 and −20.9 mV, respectively. When Ab 1 was attached, the zeta potential was −22.5 mV, indicating successful binding …”

Section: Resultsmentioning

confidence: 99%

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A Co-Reactive Immunosensor Based on Ti₃C₂T_x MXene@TiO₂–MoS₂ Hybrids Promoting luminol@Au@Ni–Co NCs Electrochemiluminescence for CYFRA 21–1 Detection

Ren,

Shao,

Xie

et al. 2024

ACS Sens.

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The construction of assays is capable of accurately detecting cytokeratin-19 (CYFRA 21–1), which is critical for the rapid diagnosis of nonsmall cell lung cancer. In this work, a novel electrochemiluminescence (ECL) immunosensor based on the co-reaction promotion of luminol@Au@Ni–Co nanocages (NCs) as ECL probe by Ti3C2T x MXene@TiO2–MoS2 hybrids as co-reaction accelerator was proposed to detect CYFRA 21–1. Ni–Co NCs, as a derivative of Prussian blue analogs, can be loaded with large quantities of Au NPs, luminol, and CYFRA 21–1 secondary antibodies due to their high specific surface area. To further improve the sensitivity of the developed ECL immunosensor, Ti3C2T x MXene@TiO2–MoS2 hybrids were prepared by in situ growth of TiO2 nanosheets on highly conductive Ti3C2T x MXene, and MoS2 was homogeneously grown on Ti3C2T x MXene@TiO2 surfaces by the hydrothermal method. Ti3C2T x MXene@TiO2–MoS2 hybrids possess excellent catalytic performance on the electro-redox of H2O2 generating more O2 ·– and obtaining optimal ECL intensity of the luminol/H2O2 system. Under the appropriate experimental conditions, the quantitative detection range of CYFRA 21–1 was from 0.1 pg mL–1 to 100 ng mL–1, and the limit of detection (LOD) was 0.046 pg mL–1. The present sensor has a lower LOD with a wider linear range, which provides a new analytical assay for the early diagnosis of small-cell-type lung cancer labels.

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Section: Resultsmentioning

confidence: 99%

“…When Ab 1 was attached, the zeta potential was −22.5 mV, indicating successful binding. 35 Exploration of the ECL Mechanism. Based on the above study, the luminescence mechanism of the constructed ECL immunosensor was further explored.…”

mentioning

confidence: 99%

A Co-Reactive Immunosensor Based on Ti₃C₂T_x MXene@TiO₂–MoS₂ Hybrids Promoting luminol@Au@Ni–Co NCs Electrochemiluminescence for CYFRA 21–1 Detection

Ren,

Shao,

Xie

et al. 2024

ACS Sens.

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“…Compared with noble metallic Au, Pt, and Ag NPs, , various binary metal composites of Au@Ag, Au@Pt, and Au@Pd with core–shell structures featured greatly improved catalase-like profiles. − However, mono-noble metals or hybrids nanozymes might suffer from high cost, low heterogeneity, and insufficient catalytic activity. Thus, many research attempts have been made toward the synthesis of bimetallic nanoalloys showing superb catalase-like properties, such as PtCo, PtCu, PdIr, BiAu, and BiPt. − Due to strong metal–metal interactions and extra stability, these alloy nanozymes possessed excellent mimic catalytic activity distinct from mono-metal catalysts with useful biosensing and bioanalysis applicability. , Nevertheless, little attention has been received in exploring the enzyme-mimic behavior of non-noble metal-based bimetallic NPs (e.g., FeCo alloy) as catalase nanozymes for sensor constructions.…”

Section: Introductionmentioning

confidence: 99%

Cooperative Amplification of Au@FeCo as Mimetic Catalytic Nanozymes and Bicycled Hairpin Assembly for Ultrasensitive Electrochemical Biosensing

et al. 2023

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Exploring the cooperative amplification of peroxidase-like metal nanocomposites and cycled hairpin assembly is intriguing for sensitive bioanalysis. Herein, we report the first design of a unique electrochemical biosensor based on mimicking Au@FeCo nanozymes and bicycled hairpin assembly (BHA) for synergistic signal amplification. By loading the enzyme-like FeCo alloy in Au nanoparticles (AuNPs), the as-synthesized Au@FeCo hybrids display great improvement of electronic conductivity and active surface area and excellent mimic catalase activity to H2O2 decomposition into •OH radicals. The immobilization of Au@FeCo in an electrode sensing interface is stabilized via the resulting electrodeposition in HAuCl4 while efficiently accelerating the electron transfer of electroactive ferrocene (Fc). Upon the immobilization of a helping hairpin (HH) via Au–S bonds, a specific DNA trigger (T*) is introduced to activate BHA operation through competitive strand displacement reactions among recognizing hairpin (RH), signaling hairpin (SH), and HH. T* and RH are rationally released to catalyze two cycles, in which the transient depletion of dsDNA intermediates rapidly drives the progressive hairpin assemblies to output more products SH·HH. Thus, the efficient amplification of Au@FeCo mimic catalase activity combined with BHA leads to a significantly increased current signal of Fc dependent on miRNA-21 analogous to T*, thereby directing the creation of a highly sensitive electrochemical biosensor having applicable potential in actual samples.

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“…[7] Hydrogen peroxide (H 2 O 2 ) and dissolved oxygen (O 2 ) are common luminol coreactants that can decompose in alkaline solutions to generate ROS to enhance the ECL emission of luminol. [8][9][10] In addition, the addition of various efficient co-reaction promoters further enhanced the ECL strength of luminol. [11,12] Lai et al utilized Mn SANE as an efficient co-reaction promoter to activate H 2 O 2 to generate large amounts of ROS and significantly enhance the ECL emission of luminol.…”

Section: Introductionmentioning

confidence: 99%

NiCo‐LDH Hollow Nanocage Oxygen Evolution Reaction Promotes Luminol Electrochemiluminescence

Liang,

Wang,

et al. 2023

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Conventional luminol co‐reactant electrochemiluminescence (ECL) systems suffer from low stability and accuracy due to factors such as the ease of decomposition of hydrogen peroxide and inefficient generation of reactive oxygen species (ROS) from dissolved oxygen. Inspired by the luminol ECL mechanism mediated by oxygen evolution reaction (OER), the nickel‐cobalt layered double hydroxide (NiCo‐LDH) hollow nanocages with hollow structure and defect state are used as co‐reaction promoters to enhance the ECL emission from the luminol‐H2O system. Thanks to the hollow structure and defect state, NiCo‐LDH hollow nanocages show excellent OER catalytic activity, which can stabilize and efficiently produce ROS and enhance the ECL emission. Additionally, mechanistic exploration suggests that the ROS involved in the co‐reaction of the luminol‐H2O system are derived from the OER reaction process, and there is a positive correlation between ECL intensity and the OER catalytic activity of the co‐reaction promoter. The selection of catalysts with excellent OER catalytic activity is a key factor in improving ECL emission. Finally, a dual‐mode immunosensor is constructed for the detection and analysis of alpha‐fetoprotein (AFP) based on the promoting effect of NiCo‐LDH hollow nanocages on the luminol‐H2O ECL system.

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Signal-off electrochemiluminescence immunosensor based on Mn-Eumelanin coordination nanoparticles quenching PtCo-CuFe2O4-reduced graphene oxide enhanced luminol

Cited by 12 publications

References 29 publications

A Co-Reactive Immunosensor Based on Ti₃C₂T_x MXene@TiO₂–MoS₂ Hybrids Promoting luminol@Au@Ni–Co NCs Electrochemiluminescence for CYFRA 21–1 Detection

A Co-Reactive Immunosensor Based on Ti₃C₂T_x MXene@TiO₂–MoS₂ Hybrids Promoting luminol@Au@Ni–Co NCs Electrochemiluminescence for CYFRA 21–1 Detection

Cooperative Amplification of Au@FeCo as Mimetic Catalytic Nanozymes and Bicycled Hairpin Assembly for Ultrasensitive Electrochemical Biosensing

NiCo‐LDH Hollow Nanocage Oxygen Evolution Reaction Promotes Luminol Electrochemiluminescence

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Signal-off electrochemiluminescence immunosensor based on Mn-Eumelanin coordination nanoparticles quenching PtCo-CuFe2O4-reduced graphene oxide enhanced luminol

Cited by 12 publications

References 29 publications

A Co-Reactive Immunosensor Based on Ti3C2Tx MXene@TiO2–MoS2 Hybrids Promoting luminol@Au@Ni–Co NCs Electrochemiluminescence for CYFRA 21–1 Detection

A Co-Reactive Immunosensor Based on Ti3C2Tx MXene@TiO2–MoS2 Hybrids Promoting luminol@Au@Ni–Co NCs Electrochemiluminescence for CYFRA 21–1 Detection

Cooperative Amplification of Au@FeCo as Mimetic Catalytic Nanozymes and Bicycled Hairpin Assembly for Ultrasensitive Electrochemical Biosensing

NiCo‐LDH Hollow Nanocage Oxygen Evolution Reaction Promotes Luminol Electrochemiluminescence

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A Co-Reactive Immunosensor Based on Ti₃C₂T_x MXene@TiO₂–MoS₂ Hybrids Promoting luminol@Au@Ni–Co NCs Electrochemiluminescence for CYFRA 21–1 Detection

A Co-Reactive Immunosensor Based on Ti₃C₂T_x MXene@TiO₂–MoS₂ Hybrids Promoting luminol@Au@Ni–Co NCs Electrochemiluminescence for CYFRA 21–1 Detection