Signal enhancement of surface plasmon resonance-based immunoassays for the allergen detection

Huang, Hong; Ran, Pixin; Liu, Zhigang

doi:10.1016/j.snb.2007.11.050

Cited by 20 publications

(12 citation statements)

References 45 publications

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“…At 7 min of EDC/NHS activation time (Figure 3a), ~15,400 RU was obtained which was around 29% of total protein adsorbed during scouting. Huang et al (2008) reported that in amine coupling, 60 to 80% of adsorbed protein would be immobilized and they had achieved around 62.5%. In their study, the optimum conditions were scouted for two min interval and information on maximum adsorbed protein was not available.…”

Section: Variation Of Immobilization Levelmentioning

confidence: 99%

“…The choice of appropriate biorecognition elements and immobilization method are of critical impor- Ramanan et al 1681 tance with direct impact on key performance characteristics of the sensor such as sensitivity, specificity, and limit of detection (Homola, 2008). The difficulties in the determination of low molecular weight analyte (< 20 kDa) at low concentrations using SPR have been reported by Huang et al (2008). They highlighted different possible strategies that could be applied to enhance the sensitivity and used sandwhich type assay to increase the sensitivity of allergens quantification from Dermatophagoides farinae (36-40 kDa).…”

Section: Introductionmentioning

confidence: 99%

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A simple method for quantification of interferon-2b through surface plasmon resonance technique

Ramanan¹,

Ling²,

Tey³

et al. 2010

Afr. J. Biotechnol.

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A rapid and efficient immunoassay method for quantification of interferon-α2b using surface plasmon resonance was developed with BIAcore 3000 as a sensor. Two different levels of anti-interferon monoclonal antibody were immobilized onto a CM5 chip using an amine coupling method. Similar binding ratio was observed for both the ligand densities. There was no steric hindrance and loss of antibody activity even at higher ligand density (> 22,000 RU). The sensitivity of the assay was increased up to 45% with the increment in ligand density from 15,400 to 22,360 RU. The binding between interferon-α2b and anti-interferon monoclonal antibody was predominantly controlled by mass transfer rate and the relationship was found linear, ranged from 5 to 400 ng/mL. Total cycle time per analysis was less than 8 min and required only 5 µL of sample injection.

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Section: Variation Of Immobilization Levelmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A simple method for quantification of interferon-2b through surface plasmon resonance technique

Ramanan¹,

Ling²,

Tey³

et al. 2010

Afr. J. Biotechnol.

View full text Add to dashboard Cite

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“…To allow full control over the target biomarker, a non-human IgG antibody specific to one of the sensor protein channels were spiked into the human-blood derived medium and these samples were tested for the presence of the spiked species. In several measurements the primary sensor response was further enhanced with detection antibodies specific to the spiked target species, an approach similar to a sandwich assay (Huang et al 2008). The augmentation of the assay with the several reference channels and exploitation of detection antibodies allowed screening of antibodies in whole blood samples.…”

Section: Experimental Methodsmentioning

confidence: 99%

Whole blood screening of antibodies using label-free nanoparticle biophotonic array platform

Olkhov

Parker

Shaw

2012

Biosensors and Bioelectronics

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A gold nanoparticle, localised plasmon array biosensor using light scattering has been employed in the detection of allergen-specific antibodies in whole blood and sera. The array sensor was functionalized with four different allergens, cat dander (Fel d1), dust mite (Der p1), peanut allergen (Ara h1) and dog dander (Can f1) and immuno-kinetic assay was performed to detect their respective anti-allergen IgG antibodies. Specific positive responses to antibodies at a concentration of 25 nM were observed for Fel d1, Der p1, and Ara h1 allergens, while the Can f1 channel served as a reference control. The sensitivity was further enhanced using a secondary anti-IgG detection antibodies to give a limit of detection of 2 nM. The results indicate the potential for nanoparticle scattering multiplexed arrays to screen unprepared blood samples at point-of-care for assays of complex samples such as the whole blood.

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“…Such nanoparticle-enhanced SPR detection has been used for the detection of proteins (Lyon et al 1998, Pieper-Furst et al 2005, Cao and Sim 2007, Huang et al 2008, Kawaguchi et al 2008, Mitchell and Lowe 2009, Wang et al 2009) and DNA probes (He et al 2000, Bailey et al 2003, Fang et al 2006 and most recently for the study of carbohydrate-lectin interactions (Huang et al 2013). Detection limits ranging from picomolar to attomolar levels, corresponding to a 1000 to 10 7 improvement in sensitivity when compared to standard SPR have been reported utilizing in most cases sandwich-like assay formats.…”

Section: Some Examples Of Gold-nanoparticleamplified Spr Studiesmentioning

confidence: 99%

Surface plasmon resonance: signal amplification using colloidal gold nanoparticles for enhanced sensitivity

Szunerits

Spadavecchia

Boukherroub

2014

Reviews in Analytical Chemistry

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Among all the detection methods developed for bioassays, optical-based approaches are the most popular techniques. A widely used and accepted bioanalytical technique for routine characterization of molecular recognition events at a solid interface is surface plasmon resonance (SPR) spectroscopy. Compared with other techniques, SPR offers a series of advantages including rapid and real-time analysis, in situ detection, and the possibility to determine kinetic as well as thermodynamic parameters of interacting partners without the requirement of tagging one of the two partners. This method is nowadays used in academic and industrial research laboratories for drug discovery processes, safety, and quality control applications as well as for understanding bioaffinity reactions. Past studies have shown that SPR can detect approximately 100 ng/l of proteins. Currently, much effort is still invested to further improve SPR with respect to the sensitivity in the detection of low molecular weight analytes as well as in the detection limit for different analyte/ ligand reactions. This review will focus on the use of gold nanoparticles as an amplification strategy in SPR sensing. Compared to other review articles highlighting the several synthetic routes and properties of gold nanoparticles and research utilizing gold nanoparticles for various sensing strategies, this review will exclusively look at the impact of gold nanostructures for ultrasensitive SPR sensing. Signal enhancement by gold nanoparticles is caused by several effects such as surface mass increase due to enhanced surface area, larger refractive index changes by the particle mass, themselves, and electromagnetic field coupling between the plasmonic properties of the particles (localized surface plasmon resonance) and propagating plasmons. The unique optical, electronic, and catalytic properties of gold nanoparticles put them into the forefront of interest for signal amplification as will be outlined here.

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Signal enhancement of surface plasmon resonance-based immunoassays for the allergen detection

Cited by 20 publications

References 45 publications

A simple method for quantification of interferon-2b through surface plasmon resonance technique

A simple method for quantification of interferon-2b through surface plasmon resonance technique

Whole blood screening of antibodies using label-free nanoparticle biophotonic array platform

Surface plasmon resonance: signal amplification using colloidal gold nanoparticles for enhanced sensitivity

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