Signal Enhancement of Electrochemical Biomemory Device Composed of Recombinant Azurin/Gold Nanoparticle

Lee, Taek; Yoo, Seung‐Schik; Chung, Yong-Ho; Min, Junhong; Choi, Jeong‐Woo

doi:10.1002/elan.201100182

Cited by 16 publications

(11 citation statements)

References 24 publications

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“…It was assumed that the electrons generated from the redox reaction of metalloprotein were trapped in the QD structure by a mechanism similar to a hysteresis phenomenon due to the difference in band gap 18 19 . We reported signal reinforcement using conducting materials such as GNP in previous work 20 . This was based on increasing the conductivity, which resulted in fast electron transfer and quantitative augmentation of the redox-reactive metalloprotein.…”

Section: Resultsmentioning

confidence: 97%

Electrochemical Bioelectronic Device Consisting of Metalloprotein for Analog Decision Making

Chung

Lee

Yoo

et al. 2015

Sci Rep

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We demonstrate an analog type logical device that combines metalloprotein and organic/inorganic materials and can make an interactive analog decision. Myoglobin is used as a functional biomolecule to generate electrochemical signals, and its original redox signal is controlled with various mercapto-acids by the distance effect between myoglobin and a metal surface in the process of electron transfer. Controlled signals are modulated with the introduction of inorganic materials including nanoparticles and metal ions. By forming a hybrid structure with various constituents of organic/inorganic materials, several functions for signal manipulation were achieved, including enhancement, suppression, and shift. Based on the manipulated signals of biomolecules, a novel logical system for interactive decision-making processes is proposed by selectively combining different signals. Through the arrangement of various output signals, we can define interactive logical results regulated by an inherent tendency (by metalloprotein), personal experience (by organic spacer sets), and environments (by inorganic materials). As a practical application, a group decision process is presented using the proposed logical device. The proposed flexible logic process could facilitate the realization of an artificial intelligence system by mimicking the sophisticated human logic process.

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Section: Resultsmentioning

confidence: 97%

Electrochemical Bioelectronic Device Consisting of Metalloprotein for Analog Decision Making

Chung

Lee

Yoo

et al. 2015

Sci Rep

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“…Additionally, the modified substrates were cleaned with deionized water and dried under an Ar gas stream. Then, 50 mM solution of 1-Octadecanethiol was added for 6 h, and the prepared 0.2 mg/mL of gold nanoparticle solution (3 μL) was added onto the hemoglobin self-assembled substrate for 6 h. Finally, the modified substrates were cleaned with deionized water and dried under an Ar gas stream [ 33 ]. Figure 2 a–c show the schematic diagram, the optical image of fabricated micro-gap electrode with working chamber for H 2 O 2 and optical image of zoomed Au micro-gap electrode, respectively.…”

Section: Methodsmentioning

confidence: 99%

Investigation of Hemoglobin/Gold Nanoparticle Heterolayer on Micro-Gap for Electrochemical Biosensor Application

Lee

Kim

Yoon

et al. 2016

Sensors

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In the present study, we fabricated a hemoglobin/gold nanoparticle (Hb/GNP) heterolayer immobilized on the Au micro-gap to confirm H2O2 detection with a signal-enhancement effect. The hemoglobin which contained the heme group catalyzed the reduction of H2O2. To facilitate the electron transfer between hemoglobin and Au micro-gap electrode, a gold nanoparticle was introduced. The Au micro-gap electrode that has gap size of 5 µm was fabricated by conventional photolithographic technique to locate working and counter electrodes oppositely in a single chip for the signal sensitivity and reliability. The hemoglobin was self-assembled onto the Au surface via chemical linker 6-mercaptohexanoic acid (6-MHA). Then, the gold nanoparticles were adsorbed onto hemoglobin/6-MHA heterolayers by the layer-by-layer (LbL) method. The fabrication of the Hb/GNP heterolayer was confirmed by atomic force microscopy (AFM) and surface-enhanced Raman spectroscopy (SERS). The redox property and H2O2 detection of Hb/GNP on the micro-gap electrode was investigated by a cyclic voltammetry (CV) experiment. Taken together, the present results show that the electrochemical signal-enhancement effect of a hemoglobin/nanoparticle heterolayer was well confirmed on the micro-scale electrode for biosensor applications.

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“…In addition to these properties, NPs have other exceptional advantages for developing electrochemical biosensors. It is widely known that metal NPs can enhance the electron transfer reaction of biomolecules and redox molecules [44]. Accordingly, Au and magnetic NPs were used in many electrochemical biosensors, such as using the superlattice structure of Au NPs for amplification of electrochemical signals or using the magnet-based collectable property of magnetic NPs for densely collecting target molecules [45,46].…”

Section: Np-based Biosensorsmentioning

confidence: 99%

Highly Sensitive Biosensors Based on Biomolecules and Functional Nanomaterials Depending on the Types of Nanomaterials: A Perspective Review

et al. 2020

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Biosensors are very important for detecting target molecules with high accuracy, selectivity, and signal-to-noise ratio. Biosensors developed using biomolecules such as enzymes or nucleic acids which were used as the probes for detecting the target molecules were studied widely due to their advantages. For example, enzymes can react with certain molecules rapidly and selectively, and nucleic acids can bind to their complementary sequences delicately in nanoscale. In addition, biomolecules can be immobilized and conjugated with other materials by surface modification through the recombination or introduction of chemical linkers. However, these biosensors have some essential limitations because of instability and low signal strength derived from the detector biomolecules. Functional nanomaterials offer a solution to overcome these limitations of biomolecules by hybridization with or replacing the biomolecules. Functional nanomaterials can give advantages for developing biosensors including the increment of electrochemical signals, retention of activity of biomolecules for a long-term period, and extension of investigating tools by using its unique plasmonic and optical properties. Up to now, various nanomaterials were synthesized and reported, from widely used gold nanoparticles to novel nanomaterials that are either carbon-based or transition-metal dichalcogenide (TMD)-based. These nanomaterials were utilized either by themselves or by hybridization with other nanomaterials to develop highly sensitive biosensors. In this review, highly sensitive biosensors developed from excellent novel nanomaterials are discussed through a selective overview of recently reported researches. We also suggest creative breakthroughs for the development of next-generation biosensors using the novel nanomaterials for detecting harmful target molecules with high sensitivity.

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Signal Enhancement of Electrochemical Biomemory Device Composed of Recombinant Azurin/Gold Nanoparticle

Cited by 16 publications

References 24 publications

Electrochemical Bioelectronic Device Consisting of Metalloprotein for Analog Decision Making

Electrochemical Bioelectronic Device Consisting of Metalloprotein for Analog Decision Making

Investigation of Hemoglobin/Gold Nanoparticle Heterolayer on Micro-Gap for Electrochemical Biosensor Application

Highly Sensitive Biosensors Based on Biomolecules and Functional Nanomaterials Depending on the Types of Nanomaterials: A Perspective Review

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