Signal Amplification in Living Cells: A Review of microRNA Detection and Imaging

Peng, Hanyong; Newbigging, Ashley M.; Reid, Michael S.; Uppal, Jagdeesh S.; Xu, Jingyang; Zhang, Hongquan; Le, X. Chris

doi:10.1021/acs.analchem.9b04752

Cited by 160 publications

(93 citation statements)

References 181 publications

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“…A broader application of CRISPR technology to non-nucleic acid targets can be achieved by incorporating other molecular translators. 97 CRISPR-Cas systems have been used for DNA and RNA imaging in live cells, 40,41,93 but the target site is limited to repetitive sequences, because of the low copy number of DNA or RNA in one cell and the lack of sufficient signal amplication, 98 CRISPR-Cas-integrated amplication strategies allow for enhanced signal generation at a genomic locus in xed cells and overcome the limitation of CRISPR-Cas probes that could only detect and image repetitive sequences. 42 Amplication techniques using CRISPR-Cas systems have not been demonstrated to function within live cells.…”

Section: Concluding Remarks Outlook and Perspectivesmentioning

confidence: 99%

CRISPR technology incorporating amplification strategies: molecular assays for nucleic acids, proteins, and small molecules

et al. 2021

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Section: Concluding Remarks Outlook and Perspectivesmentioning

confidence: 99%

CRISPR technology incorporating amplification strategies: molecular assays for nucleic acids, proteins, and small molecules

et al. 2021

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“…1b). Furthermore, combining with signal amplication methods, [43][44][45] the proposed strategy also could be used to construct multi stimuli-fueled DNA nanodevices for biosensing and medical diagnosis.…”

Section: Resultsmentioning

confidence: 99%

Cofactor-assisted three-way DNA junction-driven strand displacement

Jia

2021

RSC Adv.

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“…Many sensitive methods based on DNA nanotechnology for miRNA detection have been developed. [ 88,89 ] Nie et al. established a low‐fouling and sensitive SPR sensor for miRNA detection by integrating DNA tetrahedron probes (DTPs) into gold film along with the catalytic enlargement of AuNPs ( Figure A).…”

Section: Dna Nanotechnology‐based Biosensorsmentioning

confidence: 99%

DNA Nanotechnology‐Based Biosensors and Therapeutics

Shen

Wang

2021

Adv Healthcare Materials

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Over the past few decades, DNA nanotechnology engenders a vast variety of programmable nanostructures utilizing Watson–Crick base pairing. Due to their precise engineering, unprecedented programmability, and intrinsic biocompatibility, DNA nanostructures cannot only interact with small molecules, nucleic acids, proteins, viruses, and cancer cells, but also can serve as nanocarriers to deliver different therapeutic agents. Such addressability innate to DNA nanostructures enables their use in various fields of biomedical applications such as biosensors and cancer therapy. This review is begun with a brief introduction of the development of DNA nanotechnology, followed by a summary of recent applications of DNA nanostructures in biosensors and therapeutics. Finally, challenges and opportunities for practical applications of DNA nanotechnology are discussed.

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Signal Amplification in Living Cells: A Review of microRNA Detection and Imaging

Cited by 160 publications

References 181 publications

CRISPR technology incorporating amplification strategies: molecular assays for nucleic acids, proteins, and small molecules

CRISPR technology incorporating amplification strategies: molecular assays for nucleic acids, proteins, and small molecules

Cofactor-assisted three-way DNA junction-driven strand displacement

DNA Nanotechnology‐Based Biosensors and Therapeutics

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