Signal amplification in electrochemical detection of buckwheat allergenic protein using field effect transistor biosensor by introduction of anionic surfactant

Hideshima, Sho; Fujita, Kazuhiko; Harada, Yoshitaka; Tsuna, Mika; Seto, Yasuhiro; Sekiguchi, Satoshi; Kuroiwa, Shigeki; Nakanishi, Tetsuya; Osaka, Tetsuya

doi:10.1016/j.sbsr.2016.01.011

Cited by 13 publications

(6 citation statements)

References 20 publications

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“…Whereas many studies have focused on modifying bacterial membrane properties to improve bio-production or drug delivery [21,22], amplifying bacterial negative charge with the purpose of achieving enhanced LOD has not been addressed. We note that, recently, anionic surfactants have been used for amplifying the signal of electrochemical field effect transistors detecting buckwheat allergenic proteins [23].…”

Section: Introductionmentioning

confidence: 98%

Sodium dodecyl sulfate decorated Legionella pneumophila for enhanced detection with a GaAs/AlGaAs nanoheterostructure biosensor

Aziziyan

Hassen

Sharma

et al. 2020

Sensors and Actuators B: Chemical

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The net electric charge associated with a bacterial strain is primarily defined by the number of available functional groups at its surface and we observed that it can determine the limit of detection of a charge-sensing biosensor. We have investigated the dynamic range of bacterial electric charge variations through binding negatively charged sodium dodecyl sulphate (SDS) molecules, with the objective of improving the detection limit of a charge-sensing GaAs/AlGaAs nanoheterostructure biosensor designed for detection of Legionella pneumophila. A twofold increased zeta potential of L. pneumophila was measured at pH 7.4 following the exposure of these bacteria to an SDS solution at 0.02 mg/mL. Subsequently, it was possible to detect SDS decorated and heatinactivated L. pneumophila at 10 3 CFU/mL. This illustrates the fundamental role of the bacterial electric charge in the operation of photocorrosion-based III-V semiconductor biochips. We discuss the mechanisms of bacterial interaction with SDS, critical aspects of decorating bacteria with this anionic surfactant and the channels responsible for charge transfer.

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Section: Introductionmentioning

confidence: 98%

Sodium dodecyl sulfate decorated Legionella pneumophila for enhanced detection with a GaAs/AlGaAs nanoheterostructure biosensor

Aziziyan

Hassen

Sharma

et al. 2020

Sensors and Actuators B: Chemical

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“…Previously, our group has published lead- and mercury-ion-selective FET sensors, which exhibit sensitivity higher than the ideal Nernst sensitivity, based on our new methodology. , In this study, we would like to prove the enhanced sensitivity can also be achieved with FET-based protein sensors. Here, we have shown the enhancement in sensitivity, which can be as high as 80 mV/decade for protein detection, and it is significantly higher than the reported sensitivity in the range of 10–30 mV/decade. − Our sensor design thus offers a technique of tuning and amplifying the sensitivity for FET biosensors. The enhancement in sensitivity reported in this work has been quantitatively correlated with the sensor design (i.e., the gap between the gate electrode and FET active area (Figure )).…”

Section: Resultsmentioning

confidence: 72%

Design and Demonstration of Tunable Amplified Sensitivity of AlGaN/GaN High Electron Mobility Transistor (HEMT)-Based Biosensors in Human Serum

et al. 2019

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We have developed a swift and simplistic protein immunoassay using aptamer functionalized AlGaN/GaN high electron mobility transistors (HEMTs). The unique design of the sensor facilitates protein detection in a physiological salt environment overcoming charge screening effects, without requiring sample preprocessing. This study reports a tunable and amplified sensitivity of solution-gated electric double layer (EDL) HEMT-based biosensors, which demonstrates significantly enhanced sensitivity by designing a smaller gap between the gate electrode and the detection, and by operating at higher gate voltage. Sensitivity is calculated by quantifying NT-proBNP, a clinical biomarker of heart failure, in buffer and untreated human serum samples. The biosensor depicts elevated sensitivity and high selectivity. Furthermore, detailed investigation of the amplified sensitivity in an increased ionic strength environment is conducted, and it is revealed that a high sensitivity of 80.54 mV/decade protein concentration can be achieved, which is much higher than that of previously reported FET biosensors. This sensor technology demonstrates immense potential in developing surface affinity sensors for clinical diagnostics.

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“…The simplest amplification technique based on negative charges came from Osaka and his colleagues who introduced sodium dodecyl sulfate (SDS), an ionic surfactant which can couple with buckwheat protein 16 (BWp16) to provide an amplifier for their FET sensors to detect this allergenic target [147,148]. Most recently, Chen and his teammates succeeded in proving the concept of aptamer as bio-amplifier for both direct and sandwich immunoassay by SiNW-FET [143].…”

Section: Aptamers As Bio-amplifiers For Fet Biosensorsmentioning

confidence: 99%

Predicting Future Prospects of Aptamers in Field-Effect Transistor Biosensors

Chen

2020

Molecules

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Aptamers, in sensing technology, are famous for their role as receptors in versatile applications due to their high specificity and selectivity to a wide range of targets including proteins, small molecules, oligonucleotides, metal ions, viruses, and cells. The outburst of field-effect transistors provides a label-free detection and ultra-sensitive technique with significantly improved results in terms of detection of substances. However, their combination in this field is challenged by several factors. Recent advances in the discovery of aptamers and studies of Field-Effect Transistor (FET) aptasensors overcome these limitations and potentially expand the dominance of aptamers in the biosensor market.

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Signal amplification in electrochemical detection of buckwheat allergenic protein using field effect transistor biosensor by introduction of anionic surfactant

Cited by 13 publications

References 20 publications

Sodium dodecyl sulfate decorated Legionella pneumophila for enhanced detection with a GaAs/AlGaAs nanoheterostructure biosensor

Sodium dodecyl sulfate decorated Legionella pneumophila for enhanced detection with a GaAs/AlGaAs nanoheterostructure biosensor

Design and Demonstration of Tunable Amplified Sensitivity of AlGaN/GaN High Electron Mobility Transistor (HEMT)-Based Biosensors in Human Serum

Predicting Future Prospects of Aptamers in Field-Effect Transistor Biosensors

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