Signal Amplification by Enzymatic Reaction in an Immunosensor Based on Localized Surface Plasmon Resonance (LSPR)

Lee, Tae Han; Lee, Seung-Woo; Jung, Jee Ae; Ahn, Jun Hyoung; Kim, Min-Gon; Shin, Yong Beom

doi:10.3390/s100302045

Cited by 31 publications

(20 citation statements)

References 16 publications

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“…As IFN-γ-antigen binds its immobilized receptor, a second biotinylated antibody binds in a traditional sandwich immunoassay scheme. Finally, an avidin protein containing the enzyme horseradish peroxidase binds to the biotinylated antibody, and catalyzes the precipitation of 4-chloro-1-napthol onto the surface yielding detection as low as 0.54 nM [ 36 ].…”

Section: The Challenge Of Improving Limit Of Detectionmentioning

confidence: 99%

Localized Surface Plasmon Resonance Biosensing: Current Challenges and Approaches

Unser

Bruzas

et al. 2015

Sensors

434

263

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Localized surface plasmon resonance (LSPR) has emerged as a leader among label-free biosensing techniques in that it offers sensitive, robust, and facile detection. Traditional LSPR-based biosensing utilizes the sensitivity of the plasmon frequency to changes in local index of refraction at the nanoparticle surface. Although surface plasmon resonance technologies are now widely used to measure biomolecular interactions, several challenges remain. In this article, we have categorized these challenges into four categories: improving sensitivity and limit of detection, selectivity in complex biological solutions, sensitive detection of membrane-associated species, and the adaptation of sensing elements for point-of-care diagnostic devices. The first section of this article will involve a conceptual discussion of surface plasmon resonance and the factors affecting changes in optical signal detected. The following sections will discuss applications of LSPR biosensing with an emphasis on recent advances and approaches to overcome the four limitations mentioned above. First, improvements in limit of detection through various amplification strategies will be highlighted. The second section will involve advances to improve selectivity in complex media through self-assembled monolayers, “plasmon ruler” devices involving plasmonic coupling, and shape complementarity on the nanoparticle surface. The following section will describe various LSPR platforms designed for the sensitive detection of membrane-associated species. Finally, recent advances towards multiplexed and microfluidic LSPR-based devices for inexpensive, rapid, point-of-care diagnostics will be discussed.

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Section: The Challenge Of Improving Limit Of Detectionmentioning

confidence: 99%

Localized Surface Plasmon Resonance Biosensing: Current Challenges and Approaches

Unser

Bruzas

et al. 2015

Sensors

434

263

View full text Add to dashboard Cite

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“…In order to detect such analytes, larger protein receptors such as antibodies have been utilized. Lee et al [153] measured amplified LSPR signal by employing enzyme-based attachment. In their work, the intermolecular binding between an enzyme and antibody target fixed on a gold nano-island (NI) resulted in a two order of magnitude increase in their detection limit.…”

Section: Sensitivity Improvement Of Lsprmentioning

confidence: 99%

Nano-bio interfaces probed by advanced optical spectroscopy: From model system studies to optical biosensors

Zhang

Han

et al. 2013

Chin. Sci. Bull.

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Research in biology and medicine is a rapidly expanding field incorporating some of the most fundamental questions concerning structure, function, and purpose. The forefront of new research demands access to advanced techniques and instrumentation capable of probing these unanswered questions. Over the past several decades, nano-scale materials and devices ranging from quasione dimensional quantum dots to two dimensional graphene sheets have been engineered and have found applications in nano-bio imaging and spectroscopy. In this review, the incorporation of nanomaterials into three influential spectroscopic and microscopic techniques including fluorescence microscopy, surface plasmon resonance, and sum frequency generation will be introduced. Fluorescence imaging has visualized nanomaterials as compliments or replacements to comparable organic fluorphores, act as a quencher for FRET-based sensing, and serve as a nanoscaffold for molecular beacons. Their versatility in coating materials makes nanomaterials an excellent targeting molecule for any cellular macromolecule or structure. In addition to the targeting capabilities of nanomaterials in fluorescence imaging, surface plasmon resonance has incorporated nanomaterials for applications in signal enhancement, selectivity of target molecules, and the development of more refined and accurate detection. Functionalized nanoparticles enhance the capabilities of sum frequency generation vibrational spectroscopy by providing unique surface chemistry which alters target molecule interactions and orientations. In summary, the incorporation of nanomaterials has greatly enhanced the field of biology and medicine and has allowed for the continual advancement of not only research but instrument development. nano-bio interfaces, optical spectroscopy, fluoroscence, SERS, SFG Citation:Zhang X X, Han X F, Wu F G, et al. Nano-bio interfaces probed by advanced optical spectroscopy: From model system studies to optical biosensors. Chin Sci Bull, 2013, 58: 25372556, doi: 10.1007/s11434-013-5700-y Nanomaterials, which size ranges (1-100 nm) coincide with fundamentally important length scales in physics, are considered to be promising building blocks for future electrical, optical and optoelectronic applications [1][2][3][4][5][6][7][8]. For example, in metals, the mean free path of an electron at room temperature is ~10-100 nm [9]; the Bohr radius of photo-excited electron-hole pairs in semiconductors is ~1-10 nm [10]; and the 1-100 nm dimension is the range over which molecules assemble into nucleic acids and proteins [11]. Nanostructures on this size scale provide a unique technology to determine many critical structure/function relationships of biological molecules at the cellular level without introducing substantial interference. Advances in nanotechnology have developed at several stages: materials, devices, and systems. A decade ago, extensive research has focused on the development of various methods to precisely control the synthesis of nanomaterials. Therefore, many differ...

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“…Accordingly, they may be used to detect label-free biomolecule interactions and to monitor the adsorption and desorption kinetics by continuous optical measurements [ 9 ]. Hence, LSPR optical fiber sensors have been widely used for biotin-streptavidin [ 10 ] and antigen-antibody interaction monitoring [ 11 ], metal ion detection, and other applications [ 12 , 13 , 14 , 15 , 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

Optimization and Application of Reflective LSPR Optical Fiber Biosensors Based on Silver Nanoparticles

Chen

Shi

et al. 2015

Sensors

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In this study, we developed a reflective localized surface plasmon resonance (LSPR) optical fiber sensor, based on silver nanoparticles (Ag NPs). To enhance the sensitivity of the LSPR optical sensor, two key parameters were optimized, the length of the sensing area and the coating time of the Ag NPs. A sensing length of 1.5 cm and a 1-h coating time proved to be suitable conditions to produce highly sensitive sensors for biosensing. The optimized sensor has a high refractive index sensitivity of 387 nm/RIU, which is much higher than that of other reported individual silver nanoparticles in solutions. Moreover, the sensor was further modified with antigen to act as a biosensor. Distinctive wavelength shifts were found after each surface modification step. In addition, the reflective LSPR optical fiber sensor has high reproducibility and stability.

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Signal Amplification by Enzymatic Reaction in an Immunosensor Based on Localized Surface Plasmon Resonance (LSPR)

Cited by 31 publications

References 16 publications

Localized Surface Plasmon Resonance Biosensing: Current Challenges and Approaches

Localized Surface Plasmon Resonance Biosensing: Current Challenges and Approaches

Nano-bio interfaces probed by advanced optical spectroscopy: From model system studies to optical biosensors

Optimization and Application of Reflective LSPR Optical Fiber Biosensors Based on Silver Nanoparticles

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