Siglec-1-positive plasmacytoid dendritic cells (pDCs) in human peripheral blood: A semi-mature and myeloid-like subset imbalanced during protective and autoimmune responses

Wilhelm, T.; Taddeo, Adriano; Winter, Oliver; Schulz, Axel; Mälzer, Julia-Nora; Domingo, Cristina; Biesen, Robert; Thiel, Andreas; Radbruch, Andreas; Hiepe, Falk; Gerl, Velia

doi:10.1016/j.clim.2015.12.001

Cited by 16 publications

(12 citation statements)

References 57 publications

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“…CD123 and CD303), the resulting pDCs were highly attenuated in their ability to induce T cell proliferation and produce T cell stimulatory ligands (e.g. IL-12), consistent with reports that found several markers splitting pDCs into those that stimulate or do not stimulate T cells (18, 20, 49–52). We thus propose that our purer pDC population corresponds more closely to the “natural interferon-producing cells (IPCs)” (21, 53).…”

Section: Discussionsupporting

confidence: 89%

Single-cell RNA-seq reveals new types of human blood dendritic cells, monocytes, and progenitors

Villani

Satija

Reynolds

et al. 2017

Science

1,907

137

2,310

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Dendritic cells (DCs) and monocytes play a central role in pathogen sensing, phagocytosis and antigen presentation and consist of multiple specialized subtypes. However, their identities and interrelationships are not fully understood. Using unbiased single-cell RNA sequencing (RNA-seq) of ~2400 cells, we identified six human DCs and four monocyte subtypes in human blood. Our study reveals: a new DC subset that shares properties with plasmacytoid DCs (pDCs) but potently activates T cells, thus redefining pDCs; a new subdivision within the CD1C+ subset of DCs; the relationship between blastic plasmacytoid DC neoplasia cells and healthy DCs; and circulating progenitor of conventional DCs (cDCs). Our revised taxonomy will enable more accurate functional and developmental analyses as well as immune monitoring in health and disease.

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Section: Discussionsupporting

confidence: 89%

Single-cell RNA-seq reveals new types of human blood dendritic cells, monocytes, and progenitors

Villani

Satija

Reynolds

et al. 2017

Science

1,907

137

2,310

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“…Patients’ pDC counts were reduced at baseline, as compared with healthy controls, had lower frequencies in case of B‐symptoms and strikingly recovered into normal ranges upon treatment completion. Indeed, it is hard to comment on the relevance of these data given that it has recently been discovered that the traditionally‐defined pDCs actually encompass distinct, even new, variants, each of which display specific functions and properties with regard to the ability of T‐cell proliferation induction and cytokine production (See et al , ; Villani et al , ; Wilhelm et al , ; Zhang et al , ).…”

Section: Discussionmentioning

confidence: 99%

Circulating dendritic cells deficiencies as a new biomarker in classical Hodgkin lymphoma

et al. 2018

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Summary No robust biomarkers have been yet validated to identify the recurrence of disease in classical Hodgkin Lymphoma (cHL) patients upon induction treatment. The relevance of the inflammatory microenvironment in cHL prompted us to investigate the key immunomodulator myeloid dendritic cells type‐1 (mDC1), type‐2 (mDC2) and plasmacytoid dendritic cells (pDC). Blood DC levels were assessed in 52 newly diagnosed patients through multiparametric flow‐cytometry. All but two patients received ABVD regimen (doxorubicin, bleomycin, vinblastine, dacarbazine). The median counts of all DC subsets were lower in cHL patients than in healthy controls (P < 0·001). Median mDC counts were inferior for the advanced vs early stage patients for both mDC1s and mDC2s (P = 0·008; P = 0·0007 respectively). Also, median mDC2 counts were reduced in case of bulky (P = 0·0004) and extra‐nodal (P = 0·046) disease. Patients with B symptoms had lower levels for mDC1s (P = 0·046), mDC2s (P = 0·009) and pDCs (P = 0·040). All the DC subtypes increased at the end of treatment in 26 patients (P < 0·001): 4·6‐fold for mDC1, 2·4‐fold for mDC2, 4·5‐fold for pDC and aligned DCs subsets with the reference frequencies and the interquartile ranges of the controls. In conclusion, DCs may contribute to the disturbed immunological interplay typical of cHL, prompting a further evaluation of their value as a potential new biomarker.

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“…Human tDCs expressed CD5 and CD81 (Zhang et al, 2017), which was also true for mouse tDCs, espe-cially CD11c high tDCs. Lastly, both CD2 and SIGLEC1/CD169, two markers that have been used to define human pDC subpopulations (Matsui et al, 2009;Wilhelm et al, 2016), were enriched in tDCs compared with other DC subsets in both species. However, CD2 was not a unique marker for tDCs, and Siglec1 was only detected in a fraction ($20%-30%) of murine tDCs.…”

Section: Transcriptome Analysis Aligns Mouse and Human Tdcsmentioning

confidence: 98%

“…One possible mechanism to enable diverse pDC functions is a division of labor among functionally distinct pDC subpopulations. pDC subpopulations have been described in mouse and human (Bjö rck et al, 2011;Hadeiba et al, 2008;Matsui et al, 2009;Wilhelm et al, 2016;Zhang et al, 2017). However, there has been a general lack of consensus on these populations.…”

Section: Introductionmentioning

confidence: 99%

Integrated Cross-Species Analysis Identifies a Conserved Transitional Dendritic Cell Population

et al. 2019

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Siglec-1-positive plasmacytoid dendritic cells (pDCs) in human peripheral blood: A semi-mature and myeloid-like subset imbalanced during protective and autoimmune responses

Cited by 16 publications

References 57 publications

Single-cell RNA-seq reveals new types of human blood dendritic cells, monocytes, and progenitors

Single-cell RNA-seq reveals new types of human blood dendritic cells, monocytes, and progenitors

Circulating dendritic cells deficiencies as a new biomarker in classical Hodgkin lymphoma

Integrated Cross-Species Analysis Identifies a Conserved Transitional Dendritic Cell Population

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