Siegesbeckia pubescens Makino inhibits Pam3CSK4-induced inflammation in RAW 264.7 macrophages through suppressing TLR1/TLR2-mediated NF-κB activation

Sang, Wei; Zhong, Zhangfeng; Linghu, Ke-Gang; Xiong, Wei; Tse, Anfernee Kai-Wing; Cheang, Wai San; Yu, Hua; Wang, Ying

doi:10.1186/s13020-018-0193-x

Cited by 26 publications

(12 citation statements)

References 38 publications

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“…However, previous reports claimed that SG could inhibit COX-2 expression in the lung tissue of ovalbumin-challenged mice. SO ( Sigesbeckia orientalis L.) or SP ( S. pubescens Makino), two other species of Sigesbeckiae herba, could significantly weaken LPS- or Pam 3 CSK 4 -induced COX-2 expression in LPS- or Pam 3 CSK 4 -induced RAW 264.7 cells [29, 30]. Moreover, SO can attenuate acute inflammation by regulating inflammatory mediators via MAPK- and NF-κB-dependent pathways.…”

Section: Discussionmentioning

confidence: 99%

Anti-inflammatory activities of Sigesbeckia glabrescens Makino: combined in vitro and in silico investigations

et al. 2019

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Background Sigesbeckia glabrescens Makino (SG) is one of the important plant origins of Sigesbeckiae herba and has been widely used for the treatment of chronic inflammatory diseases in China. However, the underlying anti-inflammatory mechanism of SG is rarely investigated and reported. There are more than 40 kinds of chemical constituents in SG, but the action of the bioactive compounds of SG is still unclear. Therefore, we aimed to systemically investigate the mechanisms behind the anti-inflammatory properties of SG by combining in vitro and in silico investigations. Methods Cytotoxicity was measured using the 3-[4,5-dimethyl-2-thiazolyl]-2,5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH) assays. Nitric oxide (NO) release was detected using the Griess assay. The secretion of pro-inflammatory cytokines and the expression of relevant proteins were assessed using ELISA kits and Western blots, respectively. Molecular docking was performed and scored using AutoDock via a comparison with the molecular docking of N-acetyl-d-glucosamine (NAG). Results In lipopolysaccharides (LPS)-stimulated macrophages, SG significantly inhibited NO, MCP-1, and IL-6 secretion; iNOS expression; and NF-κB activation but did not significantly affect MAPK signalling (p38, ERK, and JNK). Moreover, the results from the molecular docking prediction suggested that over 10 compounds in SG could likely target TLR4, p105, and p65. Conclusions These findings suggest that the anti-inflammatory effects of SG are highly related to the inactivation of NF-κB. Moreover, this study provides a novel approach to investigate the effects of herbal medicine using combined in vitro and in silico investigations.

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Section: Discussionmentioning

confidence: 99%

Anti-inflammatory activities of Sigesbeckia glabrescens Makino: combined in vitro and in silico investigations

et al. 2019

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“…The total proteins were extracted from the harvested cells using radioimmunoprecipitation assay buffer containing 1 mM phenylmethanesulfonyl fluoride and protease inhibitor cocktail. Relevant proteins from the network analysis were detected by western blotting analysis as previously described (Sang et al, 2018). Immunofluorescence staining was used to detect the expression of inducible nitric oxide synthase (iNOS) and the translocation of nuclear factor kappa B (NF-κB) p65 (Li et al, 2018).…”

Section: Methodsmentioning

confidence: 99%

Deciphering the Pharmacological Mechanisms of the Huayu-Qiangshen-Tongbi Formula Through Integrating Network Pharmacology and In Vitro Pharmacological Investigation

Wang

Linghu

et al. 2019

Front. Pharmacol.

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Rheumatoid arthritis is a chronic inflammatory autoimmune disease, causing articular and extra-articular dysfunctions among patients, and it could result in irreversible joint damages or disability if untreated. A traditional Chinese medicine formula, Huayu-Qiangshen-Tongbi (HT) formula, has been observed successful in controlling rheumatoid arthritis progression in traditional Chinese medicine clinics. In this study, we conducted a systematic analysis of the HT formula with a purpose of proposing for its potential mechanism of action using network pharmacological methods. The potential targets of the formula were collected and screened according to the topological features of their protein–protein interaction network, and we subsequently validated our prediction results through in vitro experiments. We proposed that the HT formula could interfere with the bone metabolism and the inflammatory pathways of the body. The experimental validation results indicated that HT formula could exhibit anti-inflammatory effects by regulating several signaling pathways specifically the Toll-like receptor signaling pathway, phosphoinositide-3-kinase–Akt signaling pathway, hypoxia-inducible factor 1 signaling pathway, mitogen-activated protein kinase signaling pathway and activator protein 1 signaling pathway.

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“…NF-κB-induced cytokines further stimulate, activate and differentiate lamina propria immune cells, which aggravates the perpetuation of mucosal inflammation. In this review, we find that 55% of Chinese medicines having the abilities of regulating NF-κB expression in macrophages (e.g., SM934, artesunate, aesculin, gallic acid and epicatechin) repress the nucleus translocation and phosphorylation of NF-κBp65, inhibit the protein expressions and phosphorylation of NF-κB, whereas 45% (e.g., berberine, [162][163][164].…”

Section: Dss-induced Colon Tissuementioning

confidence: 99%

Targeting NF-κB pathway for treating ulcerative colitis: comprehensive regulatory characteristics of Chinese medicines

Zhao

2020

Chin Med

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Nuclear factor-kappa B (NF-κB) is a kind of multi-functional nuclear transcription factor involved in regulating gene transcription to influence pathological evolution of inflammatory and immune diseases. Numerous literature evidence that NF-κB pathway plays an essential role in pathogenic development of ulcerative colitis (UC). UC is a chronic non-specific inflammatory bowel disease, and until now, therapeutic agents for UC including aminosalicylates, corticosteroids and immune inhibitors still cannot exert satisfied effects on patients. In recent years, Chinese medicines suggest the advantages of alleviating symptoms and signs, decreasing side-effects and recurrence, whose one of mechanisms is related to regulation of NF-κB pathway. In this review, we categorize Chinese medicines according to their traditional therapeutic functions, and summarize the characteristics of Chinese medicines targeting NF-κB pathway in UC treatment. It indicates that 85 kinds of Chinese medicines' compounds and formulae can directly act on NF-κBp65; while 58 Chinese medicines' ingredients and formulae indirectly suppress NF-κBp65 by regulation of its upstream or other related pathways. Moreover, by the analysis of Chinese medicines' category based on their traditional functions, we conclude the category of dampness-drying and detoxificating medicine targeting NF-κB pathway accounts for primary status for amelioration of UC. Simultaneously, this review also contributes to the choices of Chinese medicine category and provides curative potential of Chinese medicines for clinical UC treatment.

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Siegesbeckia pubescens Makino inhibits Pam3CSK4-induced inflammation in RAW 264.7 macrophages through suppressing TLR1/TLR2-mediated NF-κB activation

Cited by 26 publications

References 38 publications

Anti-inflammatory activities of Sigesbeckia glabrescens Makino: combined in vitro and in silico investigations

Anti-inflammatory activities of Sigesbeckia glabrescens Makino: combined in vitro and in silico investigations

Deciphering the Pharmacological Mechanisms of the Huayu-Qiangshen-Tongbi Formula Through Integrating Network Pharmacology and In Vitro Pharmacological Investigation

Targeting NF-κB pathway for treating ulcerative colitis: comprehensive regulatory characteristics of Chinese medicines

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