“…The siderophores used were from the substance collection of the Institut für Biotechnologie und Wirkstoff-Forschung (IBWF). For iron depletion studies in liquid cultures, mycelia of M. grisea grown in CM were washed four times with Sundström minimal medium (MM) (Sundström, 1964;Antelo et al, 2006), transferred to Erlenmeyer flasks with Sundström MM and incubated with agitation (120 rpm) at 28°C. For iron uptake studies on solid medium (1.5% agar) a defined medium (DM) was used: 25 lg/l biotin, 10 g/l glucose, 6 g/l NaNO 3 , 0.5 g/l KCl, 0.5 g/l MgSO 4 , 1.5 g/l KH 2 PO 4 , 1.7 mg/l CoCl 2 Â 6H 2 O, 1.6 mg/l Cu-SO 4 Â 5H 2 O, Na 4 EDTA, 11 mg/l H 3 BO 3 , 5 mg/l MnCl 2 Â 4H 2 O, 1.5 mg/l Na 2 MoO 4 Â 2H 2 O, 22 mg/l ZnSO 4 Â 7H 2 O, 10 mg/l thiamine, 0.1 g/l adenine, arginine, cysteine, leucine, lysine, threonine, tryptophan, uracil, glycerol, 0.05 g/l aspartic acid, histidine, isoleucine, methionine, phenylalanine, proline, serine, tyrosine, valine, pH 6.5.…”