Sideband intensities in NMR spectra of samples spinning at the magic angle

Herzfeld, Judith; Berger, Alan E.

doi:10.1063/1.440136

Cited by 1,559 publications

(1,102 citation statements)

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“…Second dimension (from top to bottom) was a nondenaturing gradient gel electrophoresis (NDGGE; 3-30% polyacrylamide gel). After transfer to polyvinylidene difluoride membrane, it was subjected to Western blot with biotinylated mouse apoA-I antibody and developed with Streptavidin AP as described previously (24). Right, 3F-2-administered mouse plasma; left, 3F 14 -administered mouse plasma.…”

Section: Kinetics Of Plasma Turnover Of 14 C-radiolabeled Peptides-mentioning

confidence: 99%

“…The chemical shift anisotropies (CSAs) of the 31 P NMR from the phospholipid samples were determined using the solid line shape analysis routine from version 1.3 of Bruker's Topspin program. This program calculates the CSA following the Herzfeld-Berger method (24). The CSAs were determined from the 31 P NMR spectra recorded at at least three different spinning speeds, and the average result is reported.…”

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ApoA-I Mimetic Peptides with Differing Ability to Inhibit Atherosclerosis Also Exhibit Differences in Their Interactions with Membrane Bilayers

Handattu

Garber

Horn

et al. 2007

Journal of Biological Chemistry

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Two homologous apoA-I mimetic peptides, 3F-2 and 3F 14 , differ in their in vitro antiatherogenic properties (Epand, R. M., Epand, R. F., Sayer, B. G., Datta, G., Chaddha, M., and Anantharamaiah, G. M. (2004) J. Biol. Chem. 279, 51404 -51414). In the present work, we demonstrate that the peptide 3F-2, which has more potent anti-inflammatory activity in vitro when administered intraperitoneally to female apoE null mice (20 g/mouse/day) for 6 weeks, inhibits atherosclerosis (lesion area 15,800 ؎ 1000 m 2 , n ‫؍‬ 29), whereas 3F 14 does not (lesion area 20,400 ؎ 1000 m 2 , n ‫؍‬ 26) compared with control saline administered (19,900 ؎ 1400 m 2 , n ‫؍‬ 22). Plasma distribution of the peptides differs in that 3F-2 preferentially associates with high density lipoprotein, whereas 3F 14 preferentially associates with apoB-containing particles. After intraperitoneal injection of 14 C-labeled peptides, 3F 14 reaches a higher maximal concentration and has a longer half-time of elimination than 3F-2. A study of the effect of these peptides on the motional and organizational properties of phospholipid bilayers, using several NMR methods, demonstrates that the two peptides insert to different extents into membranes. 3F-2 with aromatic residues at the center of the nonpolar face partitions closer to the phospholipid head group compared with 3F 14 . In contrast, only 3F 14 affects the terminal methyl group of the acyl chain, decreasing the 2 H order parameter and at the same time also decreasing the molecular motion of this methyl group. This dual effect of 3F 14 can be explained in terms of the cross-sectional shape of the amphipathic helix. These results support the proposal that the molecular basis for the difference in the biological activities of the two peptides lies with their different interactions with membranes.

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Section: Kinetics Of Plasma Turnover Of 14 C-radiolabeled Peptides-mentioning

confidence: 99%

mentioning

confidence: 99%

ApoA-I Mimetic Peptides with Differing Ability to Inhibit Atherosclerosis Also Exhibit Differences in Their Interactions with Membrane Bilayers

Handattu

Garber

Horn

et al. 2007

Journal of Biological Chemistry

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“…(4.6). According to the result given by Herzfeld and Berger [96], the intensity o(the Nth sideband is of different order. We see that the sideband intensities converge at higher orders, and that the rate of convergence is much the same as for the sinusoidal functions.…”

Section: Resultsmentioning

confidence: 99%

Scalar operators in solid-state NMR

Sun

1991

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“…16 The local coordinate system with the principal axes is denoted here as i D 1, 2, 3. The final results are collected in Table 6.…”

Section: Shielding Tensor Calculationsmentioning

confidence: 99%

Raman, infrared and ¹³C NMR studies on betaine–sulfamic acid (2:1) crystal and its hydrogen bonds

Ilczyszyn

2003

J Raman Spectroscopy

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The betaine-sulfamic acid (2 : 1) crystal complex has been investigated by infrared and Raman experiments in polarized light and by cross-polarization magic angle spinning 13 C NMR. Very short and symmetric hydrogen bonds formed by acidic protons and betaine carboxylate groups ( -COO -H -OOC -) + are characterized by the Raman tensor a (R) available from Raman symmetric stretching vibrations of the carboxylic groups and by the 13 C shielding tensor s of these groups. The principal components of the both tensors were found as to be strongly related: s ii = 93.12 + 0.125a (R) ii . The sulfamate NH 2 group reorientation was characterized by Raman bandshape analysis at different temperatures. The energy of very weak O· · ·H -N -H· · ·O hydrogen bonds between the sulfamate and betaine moieties was characterized in this way.

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Sideband intensities in NMR spectra of samples spinning at the magic angle

Cited by 1,559 publications

References 7 publications

ApoA-I Mimetic Peptides with Differing Ability to Inhibit Atherosclerosis Also Exhibit Differences in Their Interactions with Membrane Bilayers

ApoA-I Mimetic Peptides with Differing Ability to Inhibit Atherosclerosis Also Exhibit Differences in Their Interactions with Membrane Bilayers

Scalar operators in solid-state NMR

Raman, infrared and ¹³C NMR studies on betaine–sulfamic acid (2:1) crystal and its hydrogen bonds

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Sideband intensities in NMR spectra of samples spinning at the magic angle

Cited by 1,559 publications

References 7 publications

ApoA-I Mimetic Peptides with Differing Ability to Inhibit Atherosclerosis Also Exhibit Differences in Their Interactions with Membrane Bilayers

ApoA-I Mimetic Peptides with Differing Ability to Inhibit Atherosclerosis Also Exhibit Differences in Their Interactions with Membrane Bilayers

Scalar operators in solid-state NMR

Raman, infrared and 13C NMR studies on betaine–sulfamic acid (2:1) crystal and its hydrogen bonds

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Raman, infrared and ¹³C NMR studies on betaine–sulfamic acid (2:1) crystal and its hydrogen bonds