Side-by-Side OCE-Study of Elasticity and SHG-Characterization of Collagen Fibers in Breast Cancer Tissue before and after Chemotherapy

Plekhanov, Anton A.; Potapov, Arseniy L.; Павлов, М. В.; Elagin, Vadim; Gubarkova, Ekaterina V.; Sovetsky, Alexander A.; Matveev, Lev A.; Vorontsov, Dmitry А.; Matveyev, Alexander L.; Vorontsov, А. Yu.; Gamayunov, S. V.; Zagaynova, Elena V.; Sirotkina, Marina A.; Zaĭtsev, V. K.; Gladkova, Natalia D.

doi:10.18287/jbpe23.09.020305

Cited by 3 publications

(2 citation statements)

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“…It is worth highlighting the research [63] in which an increase in stiffness of the extracellular matrix in human CRC cells was detected with the KRAS mutation, while with the BRAF mutation, the stiffness did not change, which is quite natural with our results. Despite the inability of C-OCE to measure the stiffness of individual cells and stromal fibers due to the maximum spatial resolution of 40-50 µm, previously, we established a correlation between the different structural arrangements of collagen bundles (by multiphoton second-harmonic generation microscopy) and breast cancer tissue stiffness (by C-OCE) before and after chemotherapy [64]. Consequently, the results obtained in the current study and discussed above [61][62][63] confirm the existence of some relationship between changes in tissue stiffness and molecular mutations of CRC and encourage comprehensive research on this topic.…”

Section: Relationship Between Tissue Stiffness and Molecular Mutation...mentioning

confidence: 84%

Tissue Elasticity as a Diagnostic Marker of Molecular Mutations in Morphologically Heterogeneous Colorectal Cancer

Plekhanov,

Kozlov,

Shepeleva

et al. 2024

IJMS

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The presence of molecular mutations in colorectal cancer (CRC) is a decisive factor in selecting the most effective first-line therapy. However, molecular analysis is routinely performed only in a limited number of patients with remote metastases. We propose to use tissue stiffness as a marker of the presence of molecular mutations in CRC samples. For this purpose, we applied compression optical coherence elastography (C-OCE) to calculate stiffness values in regions corresponding to specific CRC morphological patterns (n = 54). In parallel to estimating stiffness, molecular analysis from the same zones was performed to establish their relationships. As a result, a high correlation between the presence of KRAS/NRAS/BRAF driver mutations and high stiffness values was revealed regardless of CRC morphological pattern type. Further, we proposed threshold stiffness values for label-free targeted detection of molecular alterations in CRC tissues: for KRAS, NRAS, or BRAF driver mutation—above 803 kPa (sensitivity—91%; specificity—80%; diagnostic accuracy—85%), and only for KRAS driver mutation—above 850 kPa (sensitivity—90%; specificity—88%; diagnostic accuracy—89%). To conclude, C-OCE estimation of tissue stiffness can be used as a clinical diagnostic tool for preliminary screening of genetic burden in CRC tissues.

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Section: Relationship Between Tissue Stiffness and Molecular Mutation...mentioning

confidence: 84%

Tissue Elasticity as a Diagnostic Marker of Molecular Mutations in Morphologically Heterogeneous Colorectal Cancer

Plekhanov,

Kozlov,

Shepeleva

et al. 2024

IJMS

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“…The intercellular collagen concentration and textural heterogeneity of these locations decreased, whereas the orientational orderliness of the collagen somewhat increased, according to an analysis of SHG pictures of these regions. 107 This indicated that certain molecular pathways related to epithelial-stromal interactions may be necessary for the migration of cells that induce fibrillar collagen deposition, enhanced matrix stiffness, breast density, and carcinogenesis. 108…”

Section: Extracellular Matrixmentioning

confidence: 99%