SICKLE represses photomorphogenic development of <i>Arabidopsis</i> seedlings via HY5‐ and PIF4‐mediated signaling

Li, Tao; Li, Haojie; Lian, Hongmei; Song, Pengyu; Wang, Yulong; Duan, Jie; Song, Zhaoqing; Cao, Yan; Xu, Dongqing; Li, Jigang; Zhang, Huiyong

doi:10.1111/jipb.13329

Cited by 5 publications

(4 citation statements)

References 61 publications

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“…Plants have evolved a complex but sophisticated regulatory mechanism to fine-tune the activity of HY5 in regulating photomorphogenesis ( 15 , 52 ). BBXs play key roles in modulating the activity of HY5 by acting as transcriptional cofactors or inhibitors.…”

Section: Discussionmentioning

confidence: 99%

Cooperative transcriptional regulation by ATAF1 and HY5 promotes light-induced cotyledon opening in Arabidopsis thaliana

Yao,

Fang,

Qiao

et al. 2024

Sci. Signal.

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The opening of the embryonic leaves (cotyledons) as seedlings emerge from the dark soil into the light is crucial to ensure the survival of the plant. Seedlings that sprout in the dark elongate rapidly to reach light but keep their cotyledons closed. During de-etiolation, the transition from dark to light growth, elongation slows and the cotyledons open. Here, we report that the transcription factor ACTIVATING FACTOR1 (ATAF1) participates in de-etiolation and facilitates light-induced cotyledon opening. The transition from dark to light rapidly induced ATAF1 expression and ATAF1 accumulation in cotyledons. Seedlings lacking or overexpressing ATAF1 exhibited reduced or enhanced cotyledon opening, respectively, and transcriptomic analysis indicated that ATAF1 repressed the expression of genes associated with growth and cotyledon closure. The activation of the photoreceptor phytochrome A (phyA) by far-red light induced its association with the ATAF1 promoter and stimulation of ATAF1 expression. The transcription factor ELONGATED HYPOCOTYL5 (HY5), which is also activated in response far-red light, cooperated with phyA to induce ATAF1 expression. ATAF1 and HY5 interacted with one another and cooperatively repressed the expression of growth-promoting and cotyledon closure genes. Together, our study reveals a mechanism through which far-red light promotes cotyledon opening.

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Section: Discussionmentioning

confidence: 99%

Cooperative transcriptional regulation by ATAF1 and HY5 promotes light-induced cotyledon opening in Arabidopsis thaliana

Yao,

Fang,

Qiao

et al. 2024

Sci. Signal.

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“…Transcription factor PIF4 was initially characterized as a key component of phytochrome‐ and auxin‐signaling pathways (Lau and Deng, 2010; Zhao and Bao, 2021), which served in several biological processes, including young seedling hypocotyl elongation (Gao et al, 2022; Lan et al, 2022), plant thermomorphogenesis, heat response and leaf senescence (Li et al, 2021; Lee et al, 2021b), as well as in response to several phytohormones and diverse environmental cues (Choi and Oh, 2016; Huang et al, 2019; Yan et al, 2020b; Qiu et al, 2021; Xu and Zhu, 2021; Li et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

“…A previous study showed that PIF6 might be a regulator for seed dormancy as a result of alternative splicing (Penfield et al, 2010), although the detailed molecular mechanisms underlying this process are still unclear. PIF4 is a master protein involved in seedling hypocotyl elongation (Huang et al, 2015; Ma et al, 2016; Li et al, 2020), plant shade signaling (Luo et al, 2022), plant thermomorphogenesis (Lee et al, 2021b) and response to several hormones and environmental cues (Choi and Oh, 2016; Yan et al, 2020b; Xu and Zhu, 2021; Li et al, 2022). However, whether and, if so, how the PIF4‐mediated complex is involved in seed dormancy control remain unclear.…”

Section: Introductionmentioning

confidence: 99%

PIF4 interacts with ABI4 to serve as a transcriptional activator complex to promote seed dormancy by enhancing ABA biosynthesis and signaling

Luo,

Dai,

Xian

et al. 2024

JIPB

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Transcriptional regulation plays a key role in the control of seed dormancy, and many transcription factors (TFs) have been documented. However, the mechanisms underlying the interactions between different TFs within a transcriptional complex regulating seed dormancy remain largely unknown. Here, we showed that TF PHYTOCHROME‐INTERACTING FACTOR4 (PIF4) physically interacted with the abscisic acid (ABA) signaling responsive TF ABSCISIC ACID INSENSITIVE4 (ABI4) to act as a transcriptional complex to promote ABA biosynthesis and signaling, finally deepening primary seed dormancy. Both pif4 and abi4 single mutants exhibited a decreased primary seed dormancy phenotype, with a synergistic effect in the pif4/abi4 double mutant. PIF4 binds to ABI4 to form a heterodimer, and ABI4 stabilizes PIF4 at the protein level, whereas PIF4 does not affect the protein stabilization of ABI4. Subsequently, both TFs independently and synergistically promoted the expression of ABI4 and NCED6, a key gene for ABA anabolism. The genetic evidence is also consistent with the phenotypic, physiological and biochemical analysis results. Altogether, this study revealed a transcriptional regulatory cascade in which the PIF4–ABI4 transcriptional activator complex synergistically enhanced seed dormancy by facilitating ABA biosynthesis and signaling.

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“…Excessive amount of the constructed pCAMBIA1302- TaPsb28 was transformed into wild-type Arabidopsis thaliana (Columbia-0) by the floral dip method [ 49 , 50 ]. Transgenic overexpression lines were selected on MS medium containing 25 mg/L hygromycin supplementation, 2-week-old green seedlings of the T 2 generation were confirmed by PCR and the product was validated by sequencing.…”

Section: Methodsmentioning

confidence: 99%

Overexpression of the Wheat TaPsb28 Gene Enhances Drought Tolerance in Transgenic Arabidopsis

Wang

Zhang

et al. 2023

IJMS

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Psb28 is a soluble protein in the photosystem II (PSII) complex, but its role in the drought stress response of wheat remains unclear. Here, we functionally characterized the TaPsb28 gene, which positively regulates drought tolerance in wheat. When the full-length 546-bp TaPsb28 cDNA was transferred into Arabidopsis thaliana, it was located in the guard cell chloroplast around the stroma. Overexpression of TaPsb28 conferred drought tolerance, as exhibited by the increases in the survival rate. Transgenic plants maintained lower MDA content and higher chlorophyll content by inducing chlorophyll synthase (ChlG) gene transcription. The content of abscisic acid (ABA) and zeatin increased significantly in wild-type (WT) plants under drought stress, and the transcriptional expression levels of RD22, dihydroflavonol 4-reductase (DFR) and anthocyanin reductase (ANR) genes were induced, thus enhancing the contents of endogenous cyanidin, delphinidin, and proanthocyanidins. However, in transgenic plants, although anthocyanins were further aggregated, the ABA increase was inhibited, zeatin was restored to the control level under drought stress, and stomatal closure was promoted. These findings indicate ABA and zeatin have opposite synergistic effects in the process of drought tolerance caused by TaPsb28 because only after the effect of zeatin is alleviated can ABA better play its role in promoting anthocyanin accumulation and stomatal closure, thus enhancing the drought tolerance of transgenic plants. The results suggest that overexpression of TaPsb28 exerts a positive role in the drought response by influencing the functional metabolism of endogenous hormones. The understanding acquired through the research laid a foundation for further in-depth investigation of the function of TaPsb28 in drought resistance in wheat, especially its relationship with anthocyanidin accumulation.

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SICKLE represses photomorphogenic development of Arabidopsis seedlings via HY5‐ and PIF4‐mediated signaling

Cited by 5 publications

References 61 publications

Cooperative transcriptional regulation by ATAF1 and HY5 promotes light-induced cotyledon opening in Arabidopsis thaliana

Cooperative transcriptional regulation by ATAF1 and HY5 promotes light-induced cotyledon opening in Arabidopsis thaliana

PIF4 interacts with ABI4 to serve as a transcriptional activator complex to promote seed dormancy by enhancing ABA biosynthesis and signaling

Overexpression of the Wheat TaPsb28 Gene Enhances Drought Tolerance in Transgenic Arabidopsis

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