Sickle Hemoglobinopathy and Edwardsiella tarda Meningitis

Sachs, Joseph M.; Pacin, Michael; Counts, George W.

doi:10.1001/archpedi.1974.02110280117018

Cited by 16 publications

(12 citation statements)

References 10 publications

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“…This species has a broad host range, having been isolated from fresh mussels, fish, reptiles, seals, swine, and other vertebrates (5,11,26,31,32). In humans, E. tarda has been most often associated with sporadic cases of diarrhea (1,3,19,24), although a variety of extraintestinal diseases have been documented, including cellulitis, septicemia, meningitis, osteomyelitis, and hepatic abscesses (4,15,17,20,25,27,28,34). Little information is available on what virulence factors may be operative in selected E. tarda infections or what elements regulate pathogenicity or host tropisms among the three currently recognized species comprising this genus.…”

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confidence: 99%

Penetration and replication of Edwardsiella spp. in HEp-2 cells

1991

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The ability of 22 Edwardsiella strains to penetrate and replicate in cultured epithelial cells was initially evaluated by light microscopy methods and by the recovery of gentamicin-resistant (Gmr) bacteria from the Triton X-100 cell lysates of HEp-2-infected monolayers. Giemsa-stained HEp-2 cells revealed the presence of numerous internalized bacteria 3 h postinfection, often appearing as parallel rows of replicated bacteria within the cytosol and sometimes obliterating the cytoplasm because of the large numbers of bacilli present. Invasive bacteria were also sometimes found within cytoplasmic vacuoles in infected cells; thin-section electron micrographs of HEp-2-infected cells supported these conclusions. Results of light microscopy studies and cell lysate assays indicated that most Edwardsiella tarda (92%) and some Edwardsiella hoshinae strains were invasion positive on one or more occasions, while Edwardsiella ictaluri isolates were uniformly negative. HEp-2 invasion by E. tarda was a microfilament-dependent (cytochalasin B- and D-sensitive) process, with maximum numbers of Gmr CFU recorded between 3 and 6 h postinfection. The small percentage (0.01 to 1.0%) of the challenge inoculum recoverable as Gmr progeny 3 to 6 h postinfection was attributed to a strong cell-associated (not filterable) hemolysin that was produced by a majority (85%) of the E. tarda strains but not by E. ictaluri and only minimally by E. hoshinae. This cytolysin/hemolysin was responsible for the toxic effects observed in HEp-2 cells during the infection-replication process of edwardsiellae and appears to play a role in the release of internalized and replicated bacteria from infected cells. The results suggest an invasion strategy with some similarities to and differences from those of other recognized enteroinvasive pathogens.

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confidence: 99%

Penetration and replication of Edwardsiella spp. in HEp-2 cells

1991

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“…Fifteen cultures isolated from these samples belonged to 12 completely identified serotypes, among them 3 new serotypes; 11 cultures be- longed to 0 groups not previously described, and 3 were rough. DISCUSSION E. tarda has been implicated in various human illnesses: gastroenteritis (1), bacteremic and wound infections (8), peritonitis (3), and meningitis (11). It has been found in snakes and tortoises (7), frogs (13), gulls (2), sea lions and alligators (15), and swine (10).…”

Section: Resultsmentioning

confidence: 99%

Edwardsiella tarda isolated in Israel between 1961 and 1980

Sechter¹,

Shmilovitz²,

Altmann³

et al. 1983

J Clin Microbiol

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Edwardsiella tarda was isolated from patients, water tortoises (Clemmys caspica), and samples of water from Lake Kinnereth, the river Jordan, well water, and sewage water. Of the 53 isolates, 35 belonged to completely identified serotypes, among them 7 new ones. Fourteen cultures had O antigens, and one had an H antigen, different from those previously described. Three serotypes isolated from patients were also found in other sources: water tortoises, lake water, or both.

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“…In a patient with meningitis, the organism was detected in the urine, but its recovery from the urine may have been minimally associated with the patient's clinical manifestations. 9 In contrast, a report of 44 cases of E. tarda infection in Thailand showed that the organism was isolated from 13 patients with urinary tract infection, with or without other bacteria. 10 However, the authors did not provide further information on these cases, except that none of them was associated with bacteremia and that nearly all of the patients had chronically indwelling urethral catheters.…”

Section: Discussionmentioning

confidence: 96%