Sickle-Cell Anemia: A Simplified Screening Procedure

Rosenberg, Ari; Byrnes, Norma; Sexton, Leona

doi:10.1093/clinchem/18.5.489

Clinical Chemistry

1972

DOI: 10.1093/clinchem/18.5.489

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Sickle-Cell Anemia: A Simplified Screening Procedure

Ari Rosenberg

Norma Byrnes

Leona Sexton

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“…The erythrocytes were separated by centrifugation for 15 min at 482 g. The plasma portions were removed by aspiration and the packed cells were washed with isotonic phosphate buffer (310 imOsm) solution (PBS)* and centrifuged as before. The phenotypes of all erythrocyte samples were separately determined by microelectrophoresis technique on cellulose acetate (8,9) and confirmed by immunoprecipitation * Buffer mixtures: 2 separate phosphate buffer solutions were prepared as follows: 0.155 mol/l (310 ideal milliosmolar) sodium phosphate monobasic (NaH2P04) and 0.103 mol/l (310 ideal milliosmolar) sodium phosphate dibasic (Na2HP04). Isotonic phosphate buffers were prepared at pH 7.4 and 8.2 by mixing appropriate ratios of the stock solutions according to Henderson-Hasselbalch equation.…”

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confidence: 99%

An Improved Method of Harvesting Haemoglobin‐Free Human Erythrocyte Ghosts

Bhattacharya

1983

Scandinavian Journal of Haematology

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The ghosts (stromas) of human erythrocytes bearing Hb of different phenotypes were separately isolated by incubating the erythrocytes in hypotonic (20 imOsm) phosphate buffer saline (PBS) for 1 h at pH 7.4. All of these washed ghosts were found to react with benzidine indicating the presence of Hb. In the preparation of Hb free ghosts, the pH 8.2 of the phosphate buffer, incubation time (4 h) and extra oxygen supply to the medium were noticed to be very effective. The results shows that certain ghosts bound to SS, SF, and S‐Thal type of Hb were more resistant to the clearing procedure than the ghosts bound to AA, AF, ASF and AC types of Hb. The combined effect of above variables for the optimum isolation of clear ghosts with minimum or no loss of membrane components has been described.

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confidence: 99%

An Improved Method of Harvesting Haemoglobin‐Free Human Erythrocyte Ghosts

Bhattacharya

1983

Scandinavian Journal of Haematology

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The Genetics of Sickle Cell Anemia and Related Syndromes

Rucknagel¹

1974

Arch Intern Med

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Tocotrienol and fatty acid composition of barley oil and their effects on lipid metabolism

Wang

Newman

et al. 1993

Plant Food Hum Nutr

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Barley oil was extracted with hexane from the grain of a high oil waxy hull-less barley. Twelve male broiler chicks were fed corn-based diets with either 10% barley oil, 10% corn oil or 10% margarine ad libitum for ten days. Total plasma cholesterol concentration of the chicks fed barley oil was 34% lower (p < 0.05) than that of the chicks fed margarine. Plasma low density lipoprotein cholesterol concentration of chicks fed barley oil was 53% and 59% lower (p < 0.05) than those of chicks fed corn oil and margarine, respectively. Plasma high density lipoprotein cholesterol and triglyceride concentration of the barley oil group were similar to those of the margarine but higher (p < 0.05) than those of the corn oil group. Chicks fed the barley oil gained more (p < 0.05) body weight than those fed the corn oil and margarine. Barley oil had an effect in suppression of TC and LDLC in chicks compared to margarine. Barley oil suppressed LDLC but not HDLC in chicks compared to corn oil. A greater weight gain of the chicks fed barley oil suggested that these chicks had normally functioning digestion and absorption. alpha-Tocotrienol and gamma-tocotrienol content of the barley oil were 24 and 17 times greater, respectively, than those observed in the corn oil, while the same fractions were not detectable in the margarine. Polyunsaturated fatty acid content of the barley oil was more than threefold that of margarine. These data suggest that alpha-tocotrienol and polyunsaturated fatty acids are hypocholesterolemic components in barley oil.

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Sickle-Cell Anemia: A Simplified Screening Procedure

Cited by 3 publications

References 0 publications

An Improved Method of Harvesting Haemoglobin‐Free Human Erythrocyte Ghosts

An Improved Method of Harvesting Haemoglobin‐Free Human Erythrocyte Ghosts

The Genetics of Sickle Cell Anemia and Related Syndromes

Tocotrienol and fatty acid composition of barley oil and their effects on lipid metabolism

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