Sialylation sensitive bands in the Raman spectra of oligosaccharides and glycoproteins

Oleinikov, Vladimir; Kryukov, E.; Kovner, M. A.; Ermishov, Mikhail A.; Tuzikov, Alexander; Shiyan, S. D.; Bovin, Nicolai V.; Nabiev, Igor

doi:10.1016/s0022-2860(99)00016-2

Cited by 9 publications

(8 citation statements)

References 2 publications

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“…The band around 873 cm À1 on the Raman spectrum was detected in both RBC spectra (the peak appears stronger in the upper graph); this band was reported as a typical signal for other sialosides 62 as a result of the COH deformation of their glycerol part. 62 The scheme of the interactions of Au-NPs with RBCs is presented in Figure 2d. The main interactions of Au-NP aggregates and RBCs depicted in this figure reflect data obtained by Raman scattering data, Figure 2c.…”

Section: Resultsmentioning

confidence: 83%

“…Several peaks in the presented spectra can be assigned to proteins: the peak at 1109 cm –1 can be also assigned to C–C and C–N stretching, while the band around 1450 cm –1 is assigned to a CH 2 bond. The band around 873 cm –1 on the Raman spectrum was detected in both RBC spectra (the peak appears stronger in the upper graph); this band was reported as a typical signal for other sialosides as a result of the COH deformation of their glycerol part …”

Section: Resultsmentioning

confidence: 84%

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Nanoplasmonics for Dual-Molecule Release through Nanopores in the Membrane of Red Blood Cells

et al. 2012

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A nanoplasmonics-based opto-nanoporation method of creating nanopores upon laser illumination is applied for inducing diffusion and triggered release of small and large molecules from red blood cells (RBCs). The method is implemented using absorbing gold nanoparticle (Au-NP) aggregates on the membrane of loaded RBCs, which, upon near-IR laser light absorption, induce release of encapsulated molecules from selected cells. The binding of Au-NPs to RBCs is characterized by Raman spectroscopy. The process of release is driven by heating localized at nanoparticles, which impacts the permeability of the membrane by affecting the lipid bilayer and/or trans-membrane proteins. Localized heating and temperature rise around Au-NP aggregates is simulated and discussed. Research reported in this work is relevant for generating nanopores for biomolecule trafficking through polymeric and lipid membranes as well as cell membranes, while dual- and multi-molecule release is relevant for theragnostics and a wide range of therapies.

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Section: Resultsmentioning

confidence: 83%

Section: Resultsmentioning

confidence: 84%

Nanoplasmonics for Dual-Molecule Release through Nanopores in the Membrane of Red Blood Cells

et al. 2012

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“…No bands in the amide‐II characteristic spectral range (1500–1600 cm −1 ) were observed. Oleinikov et al . reported the Raman spectra of the glycosides of Neu5Ac, such as α‐methylglycoside, and proposed that the strong band at 873 cm −1 assigned to the glycerol fragment vibrations can be used as a Raman marker of SA.…”

Section: Resultsmentioning

confidence: 99%

Surface‐enhanced Raman scattering of N‐acetylneuraminic acid on silver nanoparticle surface

Vinogradova

Tlahuice‐Flores

Velázquez‐Salazar

et al. 2014

J Raman Spectroscopy

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N‐Acetylneuraminic acid (sialic acid, Neu5Ac) has recently gained interest as a potential marker for a variety of pathophysiological processes, although no Raman study has been reported for this important biomolecule. In this paper, the vibrational properties of Neu5Ac were studied by means of Raman, surface‐enhanced Raman scattering (SERS), and density functional theory calculations. By adsorption of Neu5Ac on silver nanoparticle surface, strongly enhanced Raman intensities are obtained, allowing easy measurement of small amounts of aqueous Neu5Ac (10 µl of a 10−7 m solution) utilizing low laser power and short exposure time. The mechanism of adsorption of Neu5Ac on the silver surface is discussed on the basis of the experimental and theoretical results. This study demonstrates that SERS can provide an effective tool for development of a label‐free, rapid, and sensitive optical platform for identification of Neu5Ac. Copyright © 2014 John Wiley & Sons, Ltd.

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“…24 The 655 cm -1 band which is stronger in the TC spectra can be assigned to the C-S stretch mode in cysteines 25 and is also commonly observed in the Raman spectrum of sialic acids. 26 Malignant tumor cells commonly overexpress cell surface proteins which are often glycosylated. Considering the strong distance dependence of the SERS effect, a higher density of glycoproteins on the surface of TCs, where they effectively act as spacers between the lipid bilayer and the Ag nanoparticles, can account for the relative intensities of the 722 and 655 cm -1 bands in the SERS spectra of TCs and NTCs.…”

Section: Section Biophysical Chemistrymentioning

confidence: 99%

Identification of Tumor Cells through Spectroscopic Profiling of the Cellular Surface Chemistry

Yan

Reinhard

2010

J. Phys. Chem. Lett.

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A key challenge for molecular cancer diagnostics is the identification of appropriate biomarkers and detection modalities. One target of interest is the cell surface, which is involved in important steps of cancerogenesis. Here, we explore the feasibility of a label-free spectroscopic profiling of the cell surface chemistry for the detection of tumor cells. Vibrational spectra of the cellular surfaces of tumor and nontumor breast and prostate cell lines were recorded on silver nanoparticle substrates using surface-enhanced Raman spectroscopy (SERS). The quantitative analysis of the spectra revealed characteristic differences, especially in the spectral range between 600 and 900 cm−1. The detection of tumor-cell-specific differences in the recorded SERS spectra indicates the possibility of improving the precision of current cancer detection and staging approaches through a spectroscopic profiling of cell surface cancer biomarkers.

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Sialylation sensitive bands in the Raman spectra of oligosaccharides and glycoproteins

Cited by 9 publications

References 2 publications

Nanoplasmonics for Dual-Molecule Release through Nanopores in the Membrane of Red Blood Cells

Nanoplasmonics for Dual-Molecule Release through Nanopores in the Membrane of Red Blood Cells

Surface‐enhanced Raman scattering of N‐acetylneuraminic acid on silver nanoparticle surface

Identification of Tumor Cells through Spectroscopic Profiling of the Cellular Surface Chemistry

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